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In vitro study of mouse fibroblast tumor cells with TNF coated and Alexa488 marked silica nanoparticles with an endoscopic device for real time cancer visualization

Published online by Cambridge University Press:  31 January 2011

Marion Herz ,
Andreas Rank ,
Güenter E. M. Tovar ,
Thomas Hirth ,
Dominik Kaltenbacher ,
Jan Stallkamp  and
Achim Weber
Marion Herz
Affiliation:
marion.herz@igb.fraunhofer.de, Fraunhofer-Institute for Interfacial Engineering and Biotechnology, Stuttgart, Germany
Andreas Rank
Affiliation:
andreas.Rank@igb.fraunhofer.de, Fraunhofer-Institute for Interfacial Engineering and Biotechnology, Stuttgart, Germany
Güenter E. M. Tovar
Affiliation:
guenter.tovar@igb.fraunhofer.de, Fraunhofer-Institute for Interfacial Engineering and Biotechnology, Stuttgart, Germany
Thomas Hirth
Affiliation:
Thomas.Hirth@igb.fraunhofer.de, Fraunhofer-Institute for Interfacial Engineering and Biotechnology, Stuttgart, Germany
Dominik Kaltenbacher
Affiliation:
dominik.kaltenbacher@ipa.fraunhofer.de, Fraunhofer-Institute for Manufacturing Engineering and Automation, Stuttgart, Germany
Jan Stallkamp
Affiliation:
jan.stallkamp@ipa.fraunhofer.de, Fraunhofer-Institute for Manufacturing Engineering and Automation, Stuttgart, Germany
Achim Weber
Affiliation:
achim.weber@igb.fraunhofer.de, Fraunhofer-Institute for Interfacial Engineering and Biotechnology, Stuttgart, Germany
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Abstract

Tumor resection done by minimally invasive procedure owns the challenge of a fast and reliable differentiation between healthy and tumorous tissue. We aim at investigating and developing a method for an intraoperative visualization of tumor cells with functionalized nanoparticles. The goal is to use this technique for the intraoperative use. Our so-called biohybrid systems consist of nanoparticles that are produced by Stöber synthesis and coupled with bio active proteins. Such biomimetic nanostructures are capable of imitating the effects of membrane-bound cytokines, which bind to tumor cells for labeling them. A flexible and modular test environment has been developed to evaluate the spraying properties of the particles and to study tissue probes. It enables a fast investigation of different particle configurations and spraying parameters like pressure, spray volume, nozzle geometry, etc.

Keywords

nanoscalebiomimetic (chemical reaction)biomedical
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1190: Symposium NN – Active Polymers , 2009 , 1190-NN11-23
Copyright
Copyright © Materials Research Society 2009

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References

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