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Integrating Colloidal Quantum Dots with Porous Silicon for High Sensitivity Biosensing

Published online by Cambridge University Press:  21 March 2011

Girija Gaur
Affiliation:
Department of Electrical Engineering and Computer Science, Vanderbilt University, Nashville, TN 37235, U.S.A.
Dmitry Koktysh
Affiliation:
Department of Chemistry, Vanderbilt Institute of Nanoscale Science and Engineering, Vanderbilt University, Nashville, TN 37235, USA
Sharon M. Weiss
Affiliation:
Department of Electrical Engineering and Computer Science, Vanderbilt University, Nashville, TN 37235, U.S.A.
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Abstract

We aim to utilize the high surface area of a porous silicon (PSi) matrix coupled with semiconductor quantum dot (QD) amplifiers for ultrasensitive optical detection of small biomolecules using a dual-mode detection scheme. In our system, QDs attached to the target biomolecule serve as signal amplifiers by providing an additional refractive index increase beyond that of the smaller target molecules. The strong photoluminescence (PL) from the QDs serves as a secondary indication of target molecule attachment in the pores. A resulting increase in optical thickness of ∼190 nm and detection sensitivity of ∼700 nm/RIU have been demonstrated for attachment of glutathione capped CdTe QDs in the porous silicon matrix. Reflectance and PL measurements, combined with simulations, have been used to characterize the surface area coverage of the QDs within the porous framework, which is estimated at 10% for glutathione capped CdTe QDs.

Type
Research Article
Copyright
Copyright © Materials Research Society 2011

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References

REFERENCES

[1] Rossi, A. M., et al. , “Porous silicon biosensor for detection of viruses,” Biosens. Bioelectron., vol. 23, pp. 741–745, 2007.Google Scholar
[2] Dancil, K.-P. S., et al. ., “A Porous Silicon Optical Biosensor: Detection of Reversible Binding of IgG to a Protein A-Modified Surface,” Journal of the American Chemical Society, vol. 121, pp. 7925–7930, 1999.Google Scholar
[3] Orosco, M. M., et al. ., “Real-time monitoring of enzyme activity in a mesoporous silicon double layer,” Nat Nano, vol. 4, pp. 255–258, 2009.Google Scholar
[4] Rong, G., et al. ., “Label-free porous silicon membrane waveguide for DNA sensing,Appl. Phys. Lett., vol. 93, p. 161109, 2008.Google Scholar
[5] Homola, J., et al. ., “Surface plasmon resonance sensors: review,” Sensors and Actuators B: Chemical, vol. 54, pp. 3–15, 1999.Google Scholar
[6] Nassar, A. F., et al. ., Drug Metabolism Handbook: Concepts and Applications: John Wiley & Sons, Inc., New Jersey., 2009.Google Scholar
[7] Hudson, V. M., “Rethinking cystic fibrosis pathology: the critical role of abnormal reduced glutathione (GSH) transport caused by CFTR mutation,” Free Radical Biology and Medicine, vol. 30, pp. 1440–1461, 2001.Google Scholar
[8] Rong, G., et al. ., “High sensitivity sensor based on porous silicon waveguide,” in Mat. Res. Soc. Symp. Proc., 2006, pp. 0934-I10-04.Google Scholar
[9] Pacholski, C., et al. ., “Biosensing using porous silicon double-layer interferometers: reflective interferometric fourier transform spectroscopy,” J. Am. Chem. Soc., vol. 127, pp. 11636–11645, 2005.Google Scholar
[10] Jiao, Y., et al. ., “Dual-mode sensing platform based on colloidal gold functionalized porous silicon,” Applied Physics Letters, vol. 97, pp. 153125–3, 2010.Google Scholar