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Microfabrication of Asymmetric, Homogeneous Cell-laden Hydrogel Microcapsules

Published online by Cambridge University Press:  31 January 2011

Tram T. Dang ,
Qiaobing Xu ,
Kaitlin M Bratlie ,
Esther S O'Sullivan ,
Xiao Y Chen ,
Robert Langer  and
Daniel G. Anderson
Tram T. Dang
Affiliation:
tramdang@mit.edu, Massachusetts Institute of Technology, Chemical Engineering, Cambridge, Massachusetts, United States
Qiaobing Xu
Affiliation:
qxu@mit.edu, Massachusetts Institute of Technology, Chemical Engineering, Cambridge, Massachusetts, United States
Kaitlin M Bratlie
Affiliation:
kbratlie@mit.edu, Massachusetts Institute of Technology, Chemical Engineering, Cambridge, Massachusetts, United States
Esther S O'Sullivan
Affiliation:
estherosullivan@hotmail.com, Harvard Medical School, slet Transplantation and Cell Biology, Joslin Diabetes Center, Boston, Massachusetts, United States
Xiao Y Chen
Affiliation:
ivychen@mit.edu, Massachusetts Institute of Technology, Chemical Engineering, Cambridge, Massachusetts, United States
Robert Langer
Affiliation:
rlanger@mit.edu, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States
Daniel G. Anderson
Affiliation:
dgander@mit.edu, Massachusetts Institute of Technology, David Koch Institute for Integrative Cancer Research, Cambridge, Massachusetts, United States
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Abstract

Cell encapsulation has been broadly investigated as a technology to provide immunoprotection for transplanted endocrine cells. Here we develop a new fabrication method that allows for rapid, homogenous microencapsulation of insulin-secreting cells with varying microscale geometries and asymmetrically modified surfaces. Micromolding systems were developed using polypropylene mesh, and the mesh material/surface properties associated with efficient encapsulation were identified. Cells encapsulated using these methods maintain desirable viability and preserve their ability to proliferate and secrete insulin in a glucose-responsive manner. This new cell encapsulation approach enables a practical route to an inexpensive and convenient process for the generation of cell-laden microcapsules without requiring any specialized equipment or microfabrication process.

Keywords

biomaterialbiomedicalmicrostructure
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1239: Symposium VV – Micro- and Nanoscale Processing of Biomaterials , 2009 , 1239-VV01-13
Copyright
Copyright © Materials Research Society 2010

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References

1 Orive, G. Hernàndez, R. M., Gascón, A. R., Calafiore, R. Chang, T. M. S. Vos, P. De, Hortelano, G. Hunkeler, D. Lacík, I. and Shapiro, A. M. J. Nat. Med. 9, 104–107 (2003).Google Scholar
2 Uludag, H. Vos, P. De and Tresco, P. A. Adv. Drug. Deliver Rev. 42 (1-2), 29–64 (2000).Google Scholar
3 Dulieu, C. Poncelet, D., Neufeuld, J.N, edited by Kühtreiber, W. M., Lanza, R. P. and Chick, W. L. (Birkhauser, Boston, MA, USA, 1999).Google Scholar
4 Serp, D. Cantana, E. Heinzen, C. Stockar, U. von and Marison, I. W. Biotechnol. Bioeng. 70 (1), 41–53 (2000).Google Scholar
5 Wang, T. Lacik, I. Brissová, M., Anilkumar, A. V. Prokop, A. Hunkeler, D. Green, R. Shahrokhi, K. and Powers, A. C. Nat. Biotechnol 15 (4), 358–362 (1997).Google Scholar
6 Qiu, C. Chen, M. Yan, H. and Wu, H. K. Adv. Mater 19, 1603–1607 (2007).Google Scholar
7 Fukuda, J. Khademhosseini, A. Yeo, Y. Yang, X. Yeh, J. Eng, G. Blumling, J. Wang, C. F. Kohane, D. S. and Langer, R. Biomaterials 27 (30), 5259–5267 (2006).Google Scholar
8 Yeh, J. Ling, Y. Karp, J. M. Gantz, J. Chandawarkar, A. Eng, G. Blumling, J. Iii , Langer, R. and Khademhosseini, A. Biomaterials 27 (31), 5391–5398 (2006).Google Scholar
9 Khademhosseini, A. Eng, G. Yeh, J. Fukuda, J. Blumling, J. Langer, R. and Burdick, J. A. J. Biomed. Mater. Res. A 79A (3), 522–532 (2006).Google Scholar
10 Asfari, M. Janjic, D. Meda, P. Li, G. Halban, P. A. and Wollheim, C. B. Endocrinology 130 (1), 167–178 (1992).Google Scholar
11 Merglen, A. Theander, S. Rubi, B. Chaffard, G. Wollheim, C. B. and Maechler, P. Endocrinology 145 (2), 667–678 (2004).Google Scholar
12 Du, Y. Lo, E. Ali, S. and Khademhosseini, A. Proc. Natl. Acad. Sci. USA 105 (28) (2008).Google Scholar