Hostname: page-component-cd9895bd7-q99xh Total loading time: 0 Render date: 2024-12-27T17:49:57.292Z Has data issue: false hasContentIssue false
  • English
  • Français

Micro-Topography Enhances Directional Myogenic Differentiation of Skeletal Precursor Cells

Published online by Cambridge University Press:  01 February 2011

Yi Zhao
Yi Zhao*
Affiliation:
zhao.178@osu.edu, The Ohio State University, Department of Biomedical Engineering, 294 Bevis Hall, 1080 Carmack Road, Columbus, OH, 43210, United States, (614) 247-7424, (614) 292-7301
Get access

Abstract

Skeletal muscle tissues were constructed using an in vitro model, by differentiating skeletal myoblasts using an array of linear microstructures with the medium aspect ratios. The adaptation of skeletal myoblasts has been characterized with immunoflurescence microscopy during cell proliferation and differentiation. In particular, the dependence of the alignment efficiency on the dimensions of the microstructures was studied. The morphology difference of the myotubes in the three-dimensional tissues was reported. This paper holds the promise of efficient on-chip fabrication of skeletal muscle tissues and has an important implication in direct muscle repair and muscular mechanics.

Keywords

microstructuretissuemicroelectro-mechanical (MEMS)
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1052: Symposium DD – Microelectromechanical Systems–Materials and Devices , 2007 , 1052-DD03-27
Copyright
Copyright © Materials Research Society 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

[1] Fuller, N. J, Laskey, M.A., and Elia, M., Assessment of the composition of major body regions by dual-energy X-ray absorptiometry (DEXA), with special reference to limb muscle mass. Clin. Physiol. 12, 253–266, 1992 Google Scholar
[2] Law, P.K., Goodwin, T.G., Fang, Q., Deering, M. B, Duggirala, V., Larkin, C., Florendo, J. A, Kirby, D. S, Li, H. J, Chen, M., Cell transplantation as an experimental treatment for Duchenne muscular dystrophy, Cell Transplant., 2, 485–505, 1993 Google Scholar
[3] Chargé, S. B., Rudnicki, M. A, Cellular and molecular regulation of muscle regeneration, Physiol Rev. 84(1), 209–238, 2004.Google Scholar
[4] Bach, A. D, Beier, J. P, Stern-Staeter, J., Horch, R. E, Skeletal muscle tissue engineering, J. Cell. Mol. Med. 8(4), 413–422, 2004.Google Scholar
[5] Yan, W., George, S., Fotadar, U., Tyhovych, N., Kamer, A., Yost, M.J., Price, R.L., Haggart, C.R., Holmes, J.W., and Terracio, L.. Tissue engineering of skeletal muscle, Tissue Engineering, 2007, 13(11), 2781–2790.Google Scholar
[6] Parker, K. K, Brock, A. L, Brangwynne, C., Mannix, R. J, Wand, N., Ostuni, E., Geisse, N. A, Adams, J. C, Whitesides, G. M, and Ingber, D. E, Directional control of lamellipodia extension by constraining cell shape and orienting cell tractional forces, The FASEB J., 2002, 16, 1195–1204.Google Scholar
[7] Collinsworth, A. M, Torgan, C. E, Nagda, S. N, Rajalingam, R. J, Kraus, W. E, and Truskey, G. A, Orientation and length of mammalian skeletal myocytes in response to a unidirectional stretch, Cell Tissue Res., 302(2), 243–251.Google Scholar
[8] Neumann, T., Hauschka, S. D, Sanders, J. E, Tissue engineering of skeletal muscle using polymer fiber arrays, Tissue Eng., 2003, 9(5), 995–1003.Google Scholar
[9] Huang, N. F, Thakar, R. G, Wong, M., Kim, D., Lee, R. J, Li, S., Tissue engineering of muscle on micropatterned polymer films, Conf Proc IEEE Eng Med Biol Soc. 2004, 7, 4966–4969.Google Scholar
[10] Charest, J. L, Garcia, A. J, King, W. P, Myoblast alignment and differentiation on cell culture substrates with microscale topography and model chemistries. Biomaterials, 2007, 28(13), 2202–2210.Google Scholar
[11] Das, M., Wilson, K., Molnar, P., and Hickman, J.J., Differentiation of skeletal muscle and integration of myotubes with silicon microstructures using serum-free medium and a synthetic silane substrate, Nature Protocols 2007, 2, 1795–1801.Google Scholar
[12] Lam, M. T, Sim, S., Zhu, X., and Takayama, S., The effect of continuous wavy micropatterns on silicone substrates on the alignment of skeletal muscle myoblasts and myotubes, Biomaterials, 2006, 27(24), 4340–7.Google Scholar