Hostname: page-component-586b7cd67f-gb8f7 Total loading time: 0 Render date: 2024-11-23T18:59:09.784Z Has data issue: false hasContentIssue false
  • English
  • Français

Modular Peptide-Based Hybrid Nanoprobes for Bio-Imaging and Bio-Sensing

Published online by Cambridge University Press:  09 April 2014

Banu Taktak Karaca ,
James Meyer ,
Sarah VanOosten ,
Mark Richter  and
Candan Tamerler
Banu Taktak Karaca
Affiliation:
Bioengineering Research Center (BERC) & Bioengineering Program, University of Kansas, Lawrence, KS 66045, USA Department of Molecular Biology and Genetics, Istanbul Technical University, Istanbul 34469, Turkey
James Meyer
Affiliation:
Bioengineering Research Center (BERC) & Bioengineering Program, University of Kansas, Lawrence, KS 66045, USA
Sarah VanOosten
Affiliation:
Bioengineering Research Center (BERC) & Bioengineering Program, University of Kansas, Lawrence, KS 66045, USA
Mark Richter
Affiliation:
Department of Molecular Biosciences, University of Kansas, Lawrence, KS 66045, USA
Candan Tamerler
Affiliation:
Bioengineering Research Center (BERC) & Bioengineering Program, University of Kansas, Lawrence, KS 66045, USA Department of Mechanical Engineering, University of Kansas, Lawrence, KS 66045, USA
Get access

Abstract

The self-organization of functional proteins directly onto solid materials is attractive to a wide range of biomaterials and systems that need to accommodate a biological recognition element. In such systems, inorganic binding peptides may be an essential component due to their high affinity and selective binding features onto different types of solid surfaces. This study demonstrates a peptide-enabled self-assembly technique for designing well-defined protein arrays over a metal surface. To illustrate this concept, we designed a fusion protein that simultaneously displays a red fluorescence protein (DsRed-monomer), which is highly selective for copper ions, and a gold binding peptide AuBP. The peptide tag, AuBP, self-directs the organization of DsRed-monomer protein onto a gold surface and forms arrays built upon an efficient control of the organic/inorganic interface at the molecular level. The peptide-assisted design offers a modular approach for fabrication of fluorescent-based protein arrays with copper ion sensing ability.

Keywords

adsorptionbiologicalbiomimetic (assembly)
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1621: Symposium H/C/D/J – Advances in Structures, Properties and Applications of Biological and Bioinspired Materials , 2014 , pp. 155 - 161
Copyright
Copyright © Materials Research Society 2014 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Sarikaya, M, Tamerler, C, Schwartz, DT, Baneyx, FO. Materials assembly and formation using engineered polypeptides. Annual Review of Materials Research. 2004;34:373–408.CrossRefGoogle Scholar
Meyers, MA, Chen, PY, Lin, AYM, Seki, Y. Biological materials: Structure and mechanical properties. Prog Mater Sci. 2008;53:1–206.CrossRefGoogle Scholar
Tamerler, C, Sarikaya, M. Molecular biomimetics: Genetic synthesis, assembly, and formation of materials using peptides. Mrs Bull. 2008;33:504–10.CrossRefGoogle Scholar
Fratzl, P, Weinkamer, R. Nature's hierarchical materials. Prog Mater Sci. 2007;52:1263–334.CrossRefGoogle Scholar
Willner, I. Biomaterials for sensors, fuel cells, and circuitry. Science. 2002;298:2407–8.CrossRefGoogle ScholarPubMed
Willner, I, Willner, B. Biomolecule-Based Nanomaterials and Nanostructures. Nano Lett. 2010;10:3805–15.CrossRefGoogle ScholarPubMed
Naik, RR, Stringer, SJ, Agarwal, G, Jones, SE, Stone, MO. Biomimetic synthesis and patterning of silver nanoparticles. Nat Mater. 2002;1:169–72.CrossRefGoogle ScholarPubMed
Slocik, JM, Naik, RR. Biologically programmed synthesis of bimetallic nanostructures. Adv Mater. 2006;18:1988.CrossRefGoogle Scholar
Slocik, JM, Stone, MO, Naik, RR. Synthesis of gold nanoparticles using multifunctional peptides. Small. 2005;1:1048–52.CrossRefGoogle ScholarPubMed
Briggs, BD, Knecht, MR. Nanotechnology Meets Biology: Peptide-based Methods for the Fabrication of Functional Materials. Journal of Physical Chemistry Letters. 2012;3:405–18.CrossRefGoogle ScholarPubMed
Tamerler, C, Duman, M, Oren, EE, Gungormus, M, Xiong, XR, Kacar, T, Parviz, BA, Sarikaya, M. Materials specificity and directed assembly of a gold-binding peptide. Small. 2006;2:1372–8.CrossRefGoogle ScholarPubMed
Hnilova, M, Karaca, BT, Park, J, Jia, C, Wilson, BR, Sarikaya, M, Tamerler, C. Fabrication of hierarchical hybrid structures using bio-enabled layer-by-layer self-assembly. Biotechnology and Bioengineering. 2012;109:1120–30.CrossRefGoogle ScholarPubMed
Woodbury, RG, Wendin, C, Clendenning, J, Melendez, J, Elkind, J, Bartholomew, D, Brown, S, Furlong, CE. Construction of biosensors using a gold-binding polypeptide and a miniature integrated surface plasmon resonance sensor. Biosens Bioelectron. 1998;13:1117–26.CrossRefGoogle Scholar
Coyle, BL, Rolandi, M, Baneyx, F. Carbon-Binding Designer Proteins that Discriminate between sp(2)- and sp(3)-Hybridized Carbon Surfaces. Langmuir. 2013;29:4839–46.CrossRefGoogle ScholarPubMed
Kacar, T, Zin, MT, So, C, Wilson, B, Ma, H, Gul-Karaguler, N, Jen, AKY, Sarikaya, M, Tamerler, C. Directed Self-Immobilization of Alkaline Phosphatase on Micro-Patterned Substrates Via Genetically Fused Metal-Binding Peptide. Biotechnol Bioeng. 2009;103:696–705.CrossRefGoogle ScholarPubMed
Hnilova, M, Khatayevich, D, Carlson, A, Oren, EE, Gresswell, C, Zheng, S, Ohuchi, F, Sarikaya, M, Tamerler, C. Single-step fabrication of patterned gold film array by an engineered multi-functional peptide. J Colloid Interf Sci. 2012;365:97–102.CrossRefGoogle ScholarPubMed
Haussmann, A, Milde, P, Erler, C, Eng, LM. Ferroelectric Lithography: Bottom-up Assembly and Electrical Performance of a Single Metallic Nanowire. Nano Lett. 2009;9:763–8.CrossRefGoogle ScholarPubMed
Fenter, P, Eisenberger, P, Li, J, Camillone, N, Bernasek, S, Scoles, G, Ramanarayaanan, TA, Liang, KS. Structure of CH3(CH2)17SH Self-Assembled on the Ag(111) Surface: An Incommensurate Monolayer. Langmuir. 1991;7:2013–6.CrossRefGoogle Scholar
Leong, K, Chen, YC, Masiello, DJ, Zin, MT, Hnilova, M, Ma, H, Tamerler, C, Sarikaya, M, Ginger, DS, Jen, AKY. Cooperative Near-Field Surface Plasmon Enhanced Quantum Dot Nanoarrays. Advanced Functional Materials. 2010;20:2675–82.CrossRefGoogle Scholar
Porter, MD, Bright, TB, Allara, DL, Chidsey, CED. Spontaneously Organized Molecular Assemblies .4. Structural Characterization of Normal -Alkyl, Thiol Monolayers on Gold By Optical Ellipsometry Infrared-Spectroscopy and Electrochemistry. J Am Chem Soc. 1987;109:3559–68.CrossRefGoogle Scholar
Shiba, K. Exploitation of peptide motif sequences and their use in nanobiotechnology. Current Opinion in Biotechnology. 2010;21:412–25.CrossRefGoogle ScholarPubMed
Puddu, V, Perry, CC. Peptide Adsorption on Silica Nanoparticles: Evidence of Hydrophobic Interactions. Acs Nano. 2012;6:6356–63.CrossRefGoogle ScholarPubMed
Evans, JS, Samudrala, R, Walsh, TR, Oren, EE, Tamerler, C. Molecular design of inorganic-binding polypeptides. Mrs Bull. 2008;33:514–8.CrossRefGoogle Scholar
Hnilova, M, Liu, X, Yuca, E, Jia, C, Wilson, B, Karatas, AY, Gresswell, C, Ohuchi, F, Kitamura, K, Tamerler, C. Multifunctional Protein-Enabled Patterning on Arrayed Ferroelectric Materials. Acs Appl Mater Inter. 2012;4:1865–71.CrossRefGoogle ScholarPubMed
Yuca, E, Karatas, AY, Seker, UOS, Gungormus, M, Dinler-Doganay, G, Sarikaya, M, Tamerler, C. In Vitro Labeling of Hydroxyapatite Minerals by an Engineered Protein. Biotechnol Bioeng. 2011;108:1021–30.CrossRefGoogle ScholarPubMed
Mizuno, H SA, Eli, P, Hama, H, Miyawaki, A. Red fluorescent protein from Discosoma as a fusion tag and a partner for fluorescence resonance energy transfer. Biochemistry. 2001 Feb 27;40:2502–10.CrossRefGoogle Scholar
Rahimi, Y, Goulding, A, Shrestha, S, Mirpuri, S, Deo, SK. Mechanism of copper induced fluorescence quenching of red fluorescent protein, DsRed. Biochemical and Biophysical Research Communications. 2008;370:57–61.CrossRefGoogle ScholarPubMed