Hostname: page-component-848d4c4894-2xdlg Total loading time: 0 Render date: 2024-07-03T17:24:27.969Z Has data issue: false hasContentIssue false
  • English
  • Français

MS2 Bacteriophage as a Biotemplate for Semiconductor Nanoparticle Synthesis

Published online by Cambridge University Press:  31 January 2011

Brian A. Cohen ,
Alain E. Kaloyeros  and
Magnus Bergkvist
Brian A. Cohen
Affiliation:
bcohen@uamail.albany.edu, University at Albany, College of Nanoscale Science & Engineering, Albany, New York, United States
Alain E. Kaloyeros
Affiliation:
akaloyeros@uamail.albany.edu, University at Albany, College of Nanoscale Science & Engineering, Albany, New York, United States
Magnus Bergkvist
Affiliation:
mBergkvist@uamail.albany.edu, United States
Get access

Abstract

In this work, we demonstrate the synthesis of cadmium sulfide (CdS) nanoparticles in aqueous solution with MS2 bacteriophages as a biotemplate. Bionanofabrication methods offer unique opportunities for nanomaterials synthesis, where choice of biotemplate can influence the size, shape and function of the produced material. Synthesis reactions are carried out under mild aqueous conditions, and thus hazardous organic solvents, high temperature, and extreme pH can be avoided. The MS2 bacteriophage is a potential template system for bionanofabrication of nanoscale materials due to its size and inherent physical/chemical properties. MS2 capsid proteins can be genetically modified to incorporate functional groups and sequences. Also, genomic RNA in MS2 can be degraded or replaced with other oligonucleotide sequences and still form intact capsids. In this work, we use MS2 for bio-assisted synthesis of CdS nanoparticles and adjust the amount of RNA present in the template to assess biotemplate-nanomaterial property relationships. Produced nanoparticles were probed with several techniques including UV-Vis, photoluminescence and Hi-Res TEM. Optical techniques revealed that CdS nanoparticles with predicated fluorescent properties form in the presence of MS2 and remain stable in aqueous solutions. In fact, MS2-templated CdS nanoparticles were stable at room temperature for several weeks. Presence of RNA in the capsid interior was found to have major impact on the biotemplating process. Hi-Res TEM micrographs verified the presence of nanocrystals and their association with the biotemplate. Bionanofabrication using viral templates holds promise to enable an efficient, controlled and environmentally sustainable synthesis approach for a range of important nanomaterials. The work presented here represents an additional step forward towards those goals.

Keywords

biomimetic (assembly)II-VInanoscale
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1220: Symposium BB – Green Chemistry in Research and Development of Advanced Materials , 2009 , 1220-BB05-18
Copyright
Copyright © Materials Research Society 2010

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

[1] Stebbing, S. R. Hughes, R. W. and Reynolds, P. A. “Sizing, stoichiometry and optical absorbance variations of colloidal cadmium sulphide nanoparticles,” Advances in Colloid and Interface Science, vol. 147-148, pp. 272–280, 2009/6//2008.Google Scholar
[2] Hullavarad, N. V. Hullavarad, S. S. and Karulkar, P. C. “Cadmium Sulphide (CdS) Nanotechnology: Synthesis and Applications” Journal of Nanoscience and Nanotechnology, vol. 8, pp. 3272–3299, 2008.Google Scholar
[3] Clever, H. L. Derrick, M. E. and Johnson, S. A. “The Solubility of Some Sparingly Soluble Salts of Zinc and Cadmium in Water and in Aqueous Electrolyte Solutions,” Journal of Physical and Chemical Reference Data, vol. 21, pp. 941–1004, 1992.Google Scholar
[4] Crookes-Goodson, W. J., Slocik, J. M. and Naik, R. R. “Bio-directed synthesis and assembly of nanomaterials,” Chem Soc Rev, vol. 37, pp. 2403–12, Nov 2008.Google Scholar
[5] Rumnieks, J. Ose, V. Tars, K. Dislers, A. Strods, A. Cielens, I. and Renhofa, R. “Assembly of mixed rod-like and spherical particles from group I and II RNA bacteriophage coat proteins,” Virology, vol. 391, pp. 187–194, 2009.Google Scholar
[6] Yu, R. Ren, T. Sun, K. Feng, Z. Li, G. and Li, C. “Shape-Controlled Copper Selenide Nanocubes Synthesized by an Electrochemical Crystallization Method,” The Journal of Physical Chemistry C, vol. 113, pp. 10833–10837, 2009.Google Scholar
[7] Pickett, G. G. and Peabody, D. S. “Encapsidation of heterologous RNAs by bacteriophage MS2 coat protein,” Nucleic Acids Res, vol. 21, pp. 4621–6, Sep 25 1993.Google Scholar
[8] Davis M., L. K. & Battey, J., Basic Methods in Molecular Biology, 2nd ed. Norwalk, Connecticut: Appleton & Lange, 1992.Google Scholar
[9] Hooker, J. M. Kovacs, E. W. and Francis, M. B. “Interior surface modification of bacteriophage MS2,” Journal Of The American Chemical Society, vol. 126, pp. 3718–3719, Mar 31 2004.Google Scholar