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Rapid and Sensitive Detection of Cardiac Markers in Human Serum Using Surface Acoustic Wave Immunosensor

Published online by Cambridge University Press:  17 January 2012

Joonhyung Lee ,
Youn-Suk Choi ,
Yeolho Lee ,
Hun Joo Lee ,
Jung Nam Lee ,
Sang Kyu Kim ,
Kyung Yeon Han ,
Eun Chol Cho ,
Jae Chan Park  and
Soo Suk Lee
Joonhyung Lee
Affiliation:
Bio Lab., Samsung Advanced Institute of Technology, Samsung Electronics Co., Ltd., San #14-1, Nongseo-dong, Giheung-gu, Yongin-si, Gyeonggi-do, Republic of Korea
Youn-Suk Choi
Affiliation:
Bio Lab., Samsung Advanced Institute of Technology, Samsung Electronics Co., Ltd., San #14-1, Nongseo-dong, Giheung-gu, Yongin-si, Gyeonggi-do, Republic of Korea
Yeolho Lee
Affiliation:
Bio Lab., Samsung Advanced Institute of Technology, Samsung Electronics Co., Ltd., San #14-1, Nongseo-dong, Giheung-gu, Yongin-si, Gyeonggi-do, Republic of Korea
Hun Joo Lee
Affiliation:
Bio Lab., Samsung Advanced Institute of Technology, Samsung Electronics Co., Ltd., San #14-1, Nongseo-dong, Giheung-gu, Yongin-si, Gyeonggi-do, Republic of Korea
Jung Nam Lee
Affiliation:
Bio Lab., Samsung Advanced Institute of Technology, Samsung Electronics Co., Ltd., San #14-1, Nongseo-dong, Giheung-gu, Yongin-si, Gyeonggi-do, Republic of Korea
Sang Kyu Kim
Affiliation:
Bio Lab., Samsung Advanced Institute of Technology, Samsung Electronics Co., Ltd., San #14-1, Nongseo-dong, Giheung-gu, Yongin-si, Gyeonggi-do, Republic of Korea
Kyung Yeon Han
Affiliation:
Bio Lab., Samsung Advanced Institute of Technology, Samsung Electronics Co., Ltd., San #14-1, Nongseo-dong, Giheung-gu, Yongin-si, Gyeonggi-do, Republic of Korea
Eun Chol Cho
Affiliation:
Bio Lab., Samsung Advanced Institute of Technology, Samsung Electronics Co., Ltd., San #14-1, Nongseo-dong, Giheung-gu, Yongin-si, Gyeonggi-do, Republic of Korea
Jae Chan Park
Affiliation:
Bio Lab., Samsung Advanced Institute of Technology, Samsung Electronics Co., Ltd., San #14-1, Nongseo-dong, Giheung-gu, Yongin-si, Gyeonggi-do, Republic of Korea
Soo Suk Lee
Affiliation:
Bio Lab., Samsung Advanced Institute of Technology, Samsung Electronics Co., Ltd., San #14-1, Nongseo-dong, Giheung-gu, Yongin-si, Gyeonggi-do, Republic of Korea
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Abstract

We present a rapid and sensitive surface acoustic wave (SAW) immunosensor that utilizes gold staining as a signal enhancement method. A sandwich immunoassay was performed on sensing area of the SAW sensor, which could specifically capture and detect cardiac markers (cardiac troponin I (cTnI), creatine kinase (CK)-MB, and myoglobin). The analytes in human serum were captured on gold nanoparticles (AuNPs) that were conjugated in advance with detection antibodies. Introduction of these complexes to the capture antibody-immobilized sensor surface resulted in a classic AuNP-based sandwich immunoassay format that has been used for signal amplification. In order to achieve further signal enhancement, a gold staining method was performed, which demonstrated that it is possible to obtain gold staining-mediated signal augmentation on a mass-sensitive device. The sensor response due to gold staining varied as a function of cardiac marker concentration.

Keywords

acousticbiomedicalchemical reaction
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1415: Symposium HH/II/TT – MEMS, BioMEMS and Bioelectronics–Materials and Devices , 2012 , mrsf11-1415-ii09-03
Copyright
Copyright © Materials Research Society 2012

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