Hostname: page-component-78c5997874-fbnjt Total loading time: 0 Render date: 2024-11-13T07:10:21.232Z Has data issue: false hasContentIssue false

Stability Examination of PEG Bound to Gold Nanoparticles

Published online by Cambridge University Press:  12 July 2019

Jiwen Zheng
Affiliation:
National Cancer Institute
Jeffrey Clogston
Affiliation:
National Cancer Institute
Scott McNeil
Affiliation:
National Cancer Institute
Anil Patri
Affiliation:
National Cancer Institute
Get access

Abstract

Format

This is a copy of the slides presented at the meeting but not formally written up for the volume.

Abstract

Gold nanoparticles have shown great promise in a variety of biological applications including the use in highly sensitive diagnostic assays 1,2, thermal ablation 3, radiotherapy enhancement 4, as well as drug and gene delivery 5. Such gold particles, however, suffer from losing or reducing sensitivity and selectivity due to aggregation under high ion strength of biological fluids and non-specific interaction with biomolecules, such as proteins or DNA. Poly ethylene glycol (PEG), which is known to lengthen the circulation time of biomedicines in the bloodstream, reducing the non-specific binding of proteins, and increasing efficacy and tolerability, is currently used as coating for different kind of nanoparticles to improve their stability and biocompatibility. The currently used strategy was to attach PEG moelcucles to gold nanoparticle through Au-SH chemical bonding. There is no available information, however, about how the length, conformation and attachment sites of PEG moiety affect the binding stability on gold nanoparticles, which play such critical role in retaining the solubility, while facilitating both selectivity and reactivity. In our present work we examined the stability of thiolated PEG with different length and multi-thiol anchors bound to gold nanoparticle by an assay of PEG displacement with different moities including di-thiolthreitol (DTT) and mercaptoethanol. Dynamic light scattering (DLS), Atomic force microscopy (AFM), multiangle-laser light scattering (MALS) incorporated with refractive index (RI), UV and DLS detectors were employed to characterize size, geometry, and packing density of PEG. This information will enable us to understand, optimize and control their efficacy and distribution of gold nanoparticle-based diagnostic or therapeutic agents under complex physiological environments. 1 Tkachenko, A.G.; Xie, H.; Coleman, D.; Glomm, W.; Ryan, J.; Anderson, M.F.; Franzen, S.; Feldheim, D.L. J.Am. Chem. Soc.2003,125,4700.2 Nam, J.M.; Thxton, C.S.; Mirkin, C.A.; Science 2003, 301, 1884.3 Loo, C.; Lowery, A.; Halas, N.; West, J.; Drezek, R. Nano. Lett. 2005, 5, 709.4 Hainfeld, J.F.; Slatkin, D.N.; Smilowitz, H.M.; Phys. Med. Biol. 2004, 49, N309.5 Thomas, M.; Klibanov, A.; Proc. Natl. Acad. Sci. USA 2003, 100, 9138.

Type
Slide Presentations
Copyright
Copyright © Materials Research Society 2007

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)