Hostname: page-component-78c5997874-mlc7c Total loading time: 0 Render date: 2024-11-17T22:18:25.023Z Has data issue: false hasContentIssue false
  • English
  • Français

Supramolecular Ultrathin Film Strategies for DNA Assemblies: Substrates for Optobioelectronics, Gene Therapy, and Microarrays

Published online by Cambridge University Press:  21 March 2011

Rigoberto C. Advincula ,
Yingfan Wang ,
Mi-Kyoung Park ,
Gautam Bhatia ,
Seth Stepleton ,
Cara Monroe ,
Iman Shelton ,
Wally Blanton  and
Xiaowu Fan
Rigoberto C. Advincula
Affiliation:
Department of Chemistry, University of Alabama at Birmingham Birmingham, AL 35294-1240
Yingfan Wang
Affiliation:
Department of Chemistry, University of Alabama at Birmingham Birmingham, AL 35294-1240
Mi-Kyoung Park
Affiliation:
Department of Chemistry, University of Alabama at Birmingham Birmingham, AL 35294-1240
Gautam Bhatia
Affiliation:
Department of Chemistry, University of Alabama at Birmingham Birmingham, AL 35294-1240
Seth Stepleton
Affiliation:
Department of Chemistry, University of Alabama at Birmingham Birmingham, AL 35294-1240
Cara Monroe
Affiliation:
Department of Chemistry, University of Alabama at Birmingham Birmingham, AL 35294-1240
Iman Shelton
Affiliation:
Department of Chemistry, University of Alabama at Birmingham Birmingham, AL 35294-1240
Wally Blanton
Affiliation:
Department of Chemistry, University of Alabama at Birmingham Birmingham, AL 35294-1240
Xiaowu Fan
Affiliation:
Department of Chemistry, University of Alabama at Birmingham Birmingham, AL 35294-1240
Get access

Abstract

We describe our strategies and results in the preparation of supramolecularly ordered ultrathin films of DNA assemblies using the layer-by-layer (LbL) alternate polyelectrolyte adsorption technique. The properties of DNA are intimately associated with their polyelectrolyte behavior in solution. Deposition at interfaces is governed by conformation, orientation, and charge density of these biomolecules in relation to the physisorption phenomena in oppositely charged surfaces. Thus, controlling the nature of surfaces (polymer charge density, ionic strength, other non-covalent interactions, etc.) is important in modifying the adsorption phenomena. In this work, differences in adsorption and incorporation of DNA with dyes, linear polymers and dendrimers are highlighted. A number of surface sensitive spectroscopic and microscopic techniques were used to probe the adsorption and multilayer assembly phenomena, e.g. surface plasmon resonance spectroscopy (SPS), AFM, quartz crystal microbalance (QCM) and ellipsometry. These studies are important for future applications such as the use of polycations as non-viral gene transfection vectors for drug-delivery and DNA adsorption on microarray surfaces. By combining with the alternate assembly of azobenzene and phthalocyanine dyes, we have been able to prepare optobiolelectronic substrates where the phenomena of irradiation and electrochemistry can be used to probe the ordering and response of these films.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 679: Symposium B – Molecular and Biomolecular Electronics , 2001 , B5.5
Copyright
Copyright © Materials Research Society 2001

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

1. Crystal, R., Science 270, 404–410 (1995).Google Scholar
2. Fodor, S. P. S. P. A., Science 277, 393–395 (1997).Google Scholar
3. Levicky, R., Herne, T., Tarlov, M., Satija, S. J. Am. Chem. Soc. 120, 9787–9792 (1998)Google Scholar
4. Dandliker, P., Nunez, M., Barton, J., Biochemistry 37, 6491–6502 (1998)Google Scholar
5. Decher, G., in Comprehensive Supramolecular Chemistry 9, 507 (1996).Google Scholar
6. Lvov, Y., Essler, F., Decher, G., J. Phys. Chem., 97, 13773 (1993).Google Scholar
7. Lvov, Y., Ariga, K., Ichinose, I., Kunitake, T., J. Am. Chem. Soc., 117, 6117 (1995).Google Scholar
8. Caruso, F., Möhwald, H., J. Am. Chem. Soc. 121, 6039 (1999).Google Scholar
9. Caruso, F., Niikura, K., Furlong, D., Okahata, Y., Langmuir, 13, 3427–3433 (1997).Google Scholar
10. Advincula, R., IEICE Trans. Electron. E83–C, 1104 (2000).Google Scholar
11. Kelly, S., Barton, J., Jackson, N., McPherson, L., Potter, A., Spain, E., Allen, M., M, Hill, Langmuir 14, 6781 (1998).Google Scholar
12. Sukhurov, G., Mohwald, H., Decher, G., Lvov, Y., Thin Solid Films 284, 220–223 (1996).Google Scholar
13. Dunn, B., Goodby, J., West, A., Phthalocyanine Materials: Synthesis, Structure, and Function., Cambridge University Press: New York, 1998.Google Scholar
14. Thornton, A., Bloor, D., Cross, G., Szablewski, M. Macromolecules, 30, 7600 (1997).Google Scholar
15. , Mbindyo et al. Anal. Chem., 72, 2059–2065 (2000).Google Scholar
16. Advincula, R., Park, M., Wang, Y., Blanton, W., Wallace, E. in preparation.Google Scholar