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Deconvolution of Self-encoded Polymer Beads in Random Microarrays for Antigen Biosensing by Raman Spectroscopy

Published online by Cambridge University Press:  01 February 2011

Juan P Bravo-Vasquez ,
Ramon A Alvarez-Puebla ,
David Blais ,
Ying Zhang ,
Jose Raez ,
John P Pezacki  and
Hicham Fenniri
Juan P Bravo-Vasquez
Affiliation:
Juan-Pablo.Bravo-Vasquez@nrc.ca, National Institute for Nanotechnology, Supramolecular Nano-assembling Group, 11421 Saskatchewan Drive, Edmonton, T6G 2M9, Canada, 780-641-1754
Ramon A Alvarez-Puebla
Affiliation:
alvarezr@nrc.ca, National Institute for Nanotechnology, 11421 Saskatchewan Drive, Edmonton, T6G 2M9, Canada
David Blais
Affiliation:
David.Blais@nrc-cnrc.gc.ca, Steacie Institute for Molecular Sciences, 100 Sussex Drive, Ottawa, K1A 0R6, Canada
Ying Zhang
Affiliation:
yzhang@ualberta.ca, University of Alberta, Department of Chemistry, 11227 Saskatchewan Drive, Edmonton, T6G 2G2, Canada
Jose Raez
Affiliation:
jraez@ualberta.ca, University of Alberta, Department of Chemistry, 11227 Saskatchewan Drive, Edmonton, T6G 2G2, Canada
John P Pezacki
Affiliation:
John.Pezacki@nrc-cnrc.gc.ca, Steacie Institute for Molecular Sciences, 100 Sussex Drive, Ottawa, K1A 0R6, Canada
Hicham Fenniri
Affiliation:
hicham.fenniri@ualberta.ca, National Institute for Nanotechnology, 11421 Saskatchewan Drive, Edmonton, T6G 2M9, Canada
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Abstract

Random bead microarray in which each bead has been functionalized with different biomolecules are of great interest. The process by which one would identify the location of each bead is referred to as decoding. The decoding step has played a challenging role in microarray technologies due to the increase in complexity with the increase in array size. Here we report a novel, fast and reliable method of decoding randomly assembled arrays based on polymer beads with unique spectroscopic signatures. Beads were synthesized by dispersion polymerization of a family of styrene monomers and methacrylic acid to generate a spectroscopically encoded bead library. In addition to identifying the self-encoded beads through their unique spectrum in a tetraplex experiment, Raman spectroscopy was used to monitor antibody-antigen binding events on the barcoded beads. The simplicity, versatility and rapid analysis enabled by this self-encoded bead array platform technology demonstrates its potential in high-throughput biomolecular multiplex screening.

Keywords

biomedicalpolymerRaman spectroscopy
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1024: Symposium A – Combinatorial Methods for High-Throughput Materials Science , 2007 , 1024-A04-10
Copyright
Copyright © Materials Research Society 2008

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