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Role of Scaffold Architecture and Mechanical Properties of Electrospun Scaffolds in Cell Seeding

Published online by Cambridge University Press:  31 January 2011

Nandula D. Wanasekara ,
Ming Chen ,
Vijaya B Chalivendra  and
Sankha Bhowmick
Nandula D. Wanasekara
Affiliation:
nwanasekara@umassd.edu, University of Massachusetts Dartmouth, Materials & Textiles, North Dartmouth, Massachusetts, United States
Ming Chen
Affiliation:
g_m1chen@umassd.edu, University of Massachusetts Dartmouth, Biomedical Engineering & Biotechnology, North Dartmouth, Massachusetts, United States
Vijaya B Chalivendra
Affiliation:
vchalivendra@umassd.edu, University of Massachusetts Dartmouth, Mechanical Engineering, 02747, Massachusetts, United States
Sankha Bhowmick
Affiliation:
sbhowmick@umassd.edu, University of Massachusetts Dartmouth, Mechanical Engineering & Biomedical Engineering Biotechnology, 02747, Massachusetts, United States
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Abstract

Seeding a layer of cells at specific depths within scaffolds is an important optimization parameter for bi-layer skin models. The work presented investigated the effect of fiber diameter and its mechanical property on the depth of cell seeding for electro-spun fiber scaffold. Polycaprolactone (PCL) is used to generate scaffolds that are submicron (400nm) to micron (1100nm) using electro-spinning. 3T3 fibroblasts were seeded on the electro-spun fiber scaffold mat of 50-70 microns thickness in this study. In order to investigate the effect of fiber diameter on cell migration, first, the electrospun fiber scaffold was studied for variation of mechanical properties as a function of fiber diameters. Atomic force microscopy (AFM) was used to investigate the Young’s modulus (E) values as a function of fiber diameter. It was identified that as the fiber diameter increases, the Young’s modulus values decreases considerably from 1.1GPa to 200MPa. The variation in E is correlated with cell seeding depth as a function of vacuum pressure. A higher E value led to a lower depth of cell seeding (closer to the surface) indicating that nanofibrous scaffolds offer larger resistance to cell movement compared to microfibrous scaffolds.

Keywords

fibernano-indentationscanning probe microscopy (SPM)
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1239: Symposium VV – Micro- and Nanoscale Processing of Biomaterials , 2009 , 1239-VV05-04
Copyright
Copyright © Materials Research Society 2010

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