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Silicon Microchannel Array as a Basis of Biosensor Device

Published online by Cambridge University Press:  01 February 2011

Alexander Lomzov ,
Natalia Vandysheva ,
Alexander Bublikov ,
Dmitry Pyshnyi  and
Sergei Romanov
Alexander Lomzov
Affiliation:
lomzov_alex@gorodok.netInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of RASLavrentiev Ave.8Novosibirsk n/a 630090Russian Federation
Natalia Vandysheva
Affiliation:
vandysheva@isp.nsc.ru, Institute of Semiconductor Physics, Siberian Branch of RAS, Lavrentiev Ave.13, Novosibirsk, N/A, 630090, Russian Federation
Alexander Bublikov
Affiliation:
bublikov@isp.nsc.ru, Institute of Semiconductor Physics, Siberian Branch of RAS, Lavrentiev Ave.13, Novosibirsk, n/a, 630090, Russian Federation
Dmitry Pyshnyi
Affiliation:
pyshnyi@niboch.nsc.ru, Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of RAS, Lavrentiev Ave.8, Novosibirsk, n/a, 630090, Russian Federation
Sergei Romanov
Affiliation:
romanov@isp.nsc.ru, Institute of Semiconductor Physics, Siberian Branch of RAS, Lavrentiev Ave.13, Novosibirsk, n/a, 630090, Russian Federation
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Abstract

This work shows the possibilities of using the silicon-based microchannel array (Si-MCA) as a 3D-solid support of DNA probes and studying of application of optical methods for DNA hybridization direct analysis. It was obtained that amount of immobilized oligodeoxyribonucleotides on the Si-MCA surface is enhanced with lighting of Si-MCA microchannel. The sample of 160 μm thickness succeeded in immobilizing oligonucleotides 40 times as much as the standard glass slides having the same external surface. The application of Si-MCA as a carrier of DNA allows doing direct analysis of nucleic acids in infrared region by the FTIR method that essentially simplifies creation of a biosensor based on the Si-MCA. It is indicated that using of the Si-MCA as a diffraction grating in visible and UV spectra allows simplifying the spectral analysis of biochemical reaction. It was success confidently to distinguish the mediums differing on the refractive index by dn=0.006.

Keywords

sensoroptical propertiesbiomedical
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 915: Symposium R – Nanostructured Materials and Hybrid Composites for Gas Sensors and Biomedical Applications , 2006 , 0915-R03-06
Copyright
Copyright © Materials Research Society 2006

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