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Species and population structures of Pisolithus and Scleroderma identified by combined phenotypic and genomic marker analysis

Published online by Cambridge University Press:  01 April 1999

KAREN P. SIMS ,
ROBIN SEN ,
ROY WATLING  and
PETER JEFFRIES
KAREN P. SIMS
Affiliation:
Department of Biosciences, Division of General Microbiology, Viikki Biocenter, P.O. Box 56, (Viikinkaari 9C), FIN-00014 University of Helsinki, Finland Current address: Department of Biosciences, Division of Genetics, Viikki Biocenter, P.O. Box 56, (Viikinkaari 5), FIN-00014 University of Helsinki, Finland.
ROBIN SEN
Affiliation:
Department of Biosciences, Division of General Microbiology, Viikki Biocenter, P.O. Box 56, (Viikinkaari 9C), FIN-00014 University of Helsinki, Finland
ROY WATLING
Affiliation:
Royal Botanic Garden, 20A Inverleith Row, Edinburgh, EH3 5LR, U.K.
PETER JEFFRIES
Affiliation:
Research School of Biosciences, University of Kent, Canterbury, Kent, CT2 7NJ, U.K.
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Abstract

Isolates of Pisolithus and Scleroderma species from different northern temperate and tropical geographical regions were subjected to analyses of pure culture morphology, colony growth rates, isozyme (allozyme) variation and ribosomal DNA (rDNA) restriction fragment length polymorphisms (RFLPs). Cultural characteristics enabled clear species separation of isolates and together with growth rates suggested geographically-linked intraspecific variability in the Pisolithus populations. Combined or method-specific hierarchical cluster analyses of allozyme polymorphisms and RFLPs of the fungal internal transcribed spacer (ITS) and intergenic spacer (IGS) sequences confirmed the Scleroderma species groupings and considerable geographical and host-linked variation in the Pisolithus population. Isolates of Pisolithus from the Philippines were genetically very homogeneous and distinct from less related isolates from Europe, Scandinavia and North America. Based on the ITS–RFLP and isozyme polymorphism data, the isolates investigated probably represent four different ‘species groupings’ which supports similar findings from recent taxonomic and genetic studies of Australian Pisolithus species.

Type
Research Article
Information
Mycological Research , Volume 103 , Issue 4 , April 1999 , pp. 449 - 458
Copyright
The British Mycological Society 1999

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