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Activation of prophenol oxidase in the liver fluke Fasciola gigantica Cobbold

Published online by Cambridge University Press:  06 April 2009

T. Thangaraj ,
K. Nellaiappan  and
K. Ramalingam
T. Thangaraj
Affiliation:
Department of Zoology, University of Madras, Madras 600 005, India
K. Nellaiappan
Affiliation:
Department of Zoology, University of Madras, Madras 600 005, India
K. Ramalingam
Affiliation:
Department of Zoology, University of Madras, Madras 600 005, India
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Extract

Activation of prophenol oxidase of Fasciola gigantica has been demonstrated using biochemical and electrophoretic techniques. Based on the difference in the electrophoretic pattern of the proenzyme and activated enzyme, a probable mechanism of activation of the prophenol oxidase is suggested. A role for Mehlis' gland in the activation process is suggested. The significance of the results is discussed in the light of related studies in insects.

Type
Research Article
Information
Parasitology , Volume 85 , Issue 3 , December 1982 , pp. 577 - 581
Copyright
Copyright © Cambridge University Press 1982

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References

Ashida, M. (1971). Purification and characterization of prophenol oxidase from haemolymph of the silkworm Bombyx mori. Archives of Biochemistry and Biophysics 144, 749–62.CrossRefGoogle Scholar
Ashida, M. & Dohke, K. (1980). Activation of prophenol oxidase by the activating enzyme of the silk worm Bombyx mori. Insect Biochemistry 10, 37–47.CrossRefGoogle Scholar
Bogitsh, B. J. (1970). Observations on the cytochemistry of the Mehlis' gland cells of Haematoloechus medioplexus. Journal of Parasitology 56, 1084–94.Google Scholar
Clegg, J. A. (1965). Secretion of lipoprotein by Mehlis' gland in Fasciola hepatica. Annals of the New York Academy of Science 118, 969–86.CrossRefGoogle ScholarPubMed
Hanumantha Rao, K. & Madhavi, R. (1962). Mehlis' gland complex in the cattle liver fluke Fasciola gigantica. Current Science 31, 101–2.Google Scholar
Jolley, R. L. & Mason, H. S. (1965). The multiple forms of Mushroom tyrosinase. Interconversion. Journal of Biological Chemistry 240, 1489–91.Google Scholar
Lowry, O. H., Rosebrough, N. J., Farb, A. L. & Randall, R. J. (1951). Protein measurement with folin phenol reagent. Journal of Biological Chemistry 193, 265–75.CrossRefGoogle ScholarPubMed
Nellaiappan, K. & Ramalingam, K. (1980 a). Specificity of the enzyme phenol oxidase and possible metabolic pathway of sclerotization in Paraplerurus sauridae. Journal of Parasitology 66, 217–19.CrossRefGoogle ScholarPubMed
Nellaiappan, K. & Ramalingam, K. (1980 b). Prophenol oxidase and its activation in Paraplerurus sauridae (Digenea: Hemiuridae). Molecular and Biochemical Parasitology 2, 109–12.CrossRefGoogle Scholar
Ramalingam, K. (1970). Prophenolase and the role of Mehlis' gland in helminths. Experientia 26, 828.CrossRefGoogle ScholarPubMed
Sekeris, C. E. & Mergenhagen, D. (1964). Phenol oxidase system of the blow fly Galliphora erythrocephala. Science 145, 68–9.CrossRefGoogle Scholar
Smith, I. (1968). Acrylamide gel disc electrophoresis. I. Techniques of disc electrophoresis. In Chromatographic and Electrophoretic Techniques, vol. 2 (ed. Smith, I.), pp. 365–89. London: William Heinemann.Google Scholar
Smyth, J. D. (1976). Introduction to Animal Parasitology. 2nd Ed.London, Auckland and Toronto: Hodder and Stoughton.Google Scholar