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Activity of the enzyme adenosine deaminase in serum, erythrocytes and lymphocytes of rats infected with Trypanosoma evansi

Published online by Cambridge University Press:  01 September 2010

ALEKSANDRO S. DA SILVA ,
LUZIANE P. BELLÉ ,
PAULA E. R. BITENCOURT ,
VIVIANE C. G. SOUZA ,
MARCIO M. COSTA ,
CAMILA B. OLIVEIRA ,
JEANDRE A. JAQUES ,
DANIELA B. R. LEAL ,
MARIA B. MORETTO  and
CINTHIA M. MAZZANTI
...Show all authors
ALEKSANDRO S. DA SILVA*
Affiliation:
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Brazil Department of Clinical Analysis and Toxicological, Universidade Federal de Santa Maria, Brazil
LUZIANE P. BELLÉ
Affiliation:
Department of Clinical Analysis and Toxicological, Universidade Federal de Santa Maria, Brazil
PAULA E. R. BITENCOURT
Affiliation:
Department of Clinical Analysis and Toxicological, Universidade Federal de Santa Maria, Brazil
VIVIANE C. G. SOUZA
Affiliation:
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Brazil
MARCIO M. COSTA
Affiliation:
Department of Small Animals, Universidade Federal de Santa Maria, Brazil
CAMILA B. OLIVEIRA
Affiliation:
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Brazil
JEANDRE A. JAQUES
Affiliation:
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Brazil
DANIELA B. R. LEAL
Affiliation:
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Brazil
MARIA B. MORETTO
Affiliation:
Department of Clinical Analysis and Toxicological, Universidade Federal de Santa Maria, Brazil
CINTHIA M. MAZZANTI
Affiliation:
Department of Small Animals, Universidade Federal de Santa Maria, Brazil
SONIA T. A. LOPES
Affiliation:
Department of Small Animals, Universidade Federal de Santa Maria, Brazil
SILVIA G. MONTEIRO
Affiliation:
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Brazil
*
*Corresponding author: Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Camobi – 9, Building 20, Room 4232, CEP 97105900, Santa Maria – RS, Brazil. Tel: and Fax: + 55 55 32208958. E-mail: aleksandro_ss@yahoo.com.br
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Summary

In Trypanosoma evansi infections changes in the haemogram are commonly observed, and the enzyme adenosine deaminase (ADA) plays an important role in the production and differentiation of blood cells. Thus, the aim of this study was to evaluate the activity of ADA in serum, erythrocytes and lymphocytes of rats infected with T. evansi compared to non-infected rats. Thirty adult rats were used, divided into 3 uniform groups. The animals in groups A and B were infected intraperitoneally with 2×106 trypomastigotes/rat. Rodents from group C (control group), were not-infected. Blood collection was performed on days 4 and 20 post-infection (p.i.) in order to obtain acute and chronic infection stages of disease. The blood was used to assess the activity of ADA. In the blood, reduced haematocrit and increased lymphocytes were correlated with ADA activity in erythrocytes and lymphocytes. We observed reduction of ADA activity in serum and erythrocytes in rats infected with T. evansi compared to non-infected rats (P<0·05). ADA activity in lymphocytes was decreased after 4 days, when the parasitaemia was high and increased after 20 days, when the number of circulating parasites was low. In conclusion, our results showed that the ADA activity was altered in serum, lymphocytes and erythrocytes of rats, concomitantly with haematological parameters, in experimental infection by T. evansi.

Keywords

trypanosomosisADAanaemialymphocytosisrats
Type
Research Article
Information
Parasitology , Volume 138 , Issue 2 , February 2011 , pp. 201 - 208
Copyright
Copyright © Cambridge University Press 2010

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