Hostname: page-component-cd9895bd7-dzt6s Total loading time: 0 Render date: 2024-12-24T21:36:18.952Z Has data issue: false hasContentIssue false
  • English
  • Français

Antigenic variation in Trypanosoma vivax: isolation of a serodeme

Published online by Cambridge University Press:  06 April 2009

J. D. Barry  and
H. Gathuo
J. D. Barry
Affiliation:
International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya
H. Gathuo
Affiliation:
International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya
Get access Rights & Permissions [Opens in a new window]

Summary

Studies of antigenic variation in Trypanosoma vivax have been hindered by its low infectivity to laboratory hosts and difficulties in obtaining defined cloned populations. The recent isolation of mouse-infective T. vivax has partially overcome the first problem. We have developed a highly efficient cloning technique which has been applied to the isolation of a collection of 71 clones, representing 31 distinct variable antigen types, of mouse-infective T. vivax from Nigeria. This serodeme showed no cross-reaction with several T. brucei-group serodemes, but some cross-reaction with naturally occurring Kenyan T. vivax.

Type
Research Article
Information
Parasitology , Volume 89 , Issue 1 , August 1984 , pp. 49 - 58
Copyright
Copyright © Cambridge University Press 1984

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Anonymous. (1978). Proposals for the nomenclature of salivarian trypanosomes and for the maintenance of reference collections. Bulletin of the World Health Organization 56, 467–80.Google Scholar
Barry, J. D. (1979). Capping of variable antigen on Trypanosoma brucei, and its immunological and biological significance. Journal of Cell Science 37, 287–302.CrossRefGoogle ScholarPubMed
Barry, J. D. & Hajduk, S. L. (1979). Antigenic heterogeneity of bloodstream and metacyclic forms of Trypanosoma brucei. In Pathogenicity of Trypanosomes, (ed. Losos, G. and Chouinard, A.), pp. 51–56. Ottawa: IDRC.Google Scholar
Barry, J. D., Hajduk, S. L., Vickerman, K. & Le Ray, D. (1979). Detection of multiple variable antigen types in metacyclic populations of Trypanosoma brucei. Transactions of the Royal Society of Tropical Medicine and Hygiene 73, 205–8.CrossRefGoogle ScholarPubMed
Capbern, A., Giroud, C., Baltz, T. & Mattern, P. (1977). Etude des variations antigèniques au cours de la trypanosomose expérimental du lapin. Experimental Parasitology 42, 6–13.CrossRefGoogle Scholar
Dar, F. K., Paris, J. & Wilson, A. J. (1973). Serological studies on trypanosomiasis in East Africa. IV: Comparison of antigenic types of Trypanosoma vivax group organisms. Annals of Tropical Medicine and Parasitology 67, 319–29.CrossRefGoogle ScholarPubMed
De Gee, A. L. W., Shah, S. D. & Doyle, J. J. (1979). Trypanosoma vivax: Sequence of antigenic variants in mice and goats. Experimental Parasitology 48, 352–8.CrossRefGoogle ScholarPubMed
De Gee, A. L. W., Shah, S. D. & Doyle, J. J. (1980). Trypanosoma vivax: Host influence on appearance of variable antigen types. Experimental Parasitology 51, 392–9.CrossRefGoogle Scholar
Desowitz, R. S. & Watson, H. J. C. (1952). Studies on Trypanosoma vivax. III. Observations on the maintenance of a strain in white rats. Annals of Tropical Medicine and Parasitology 46, 92–100.CrossRefGoogle ScholarPubMed
Emery, D. L., Barry, J. D. & Moloo, S. K. (1980). The appearance of Trypanosoma (Duttonella) vivax in lymph following challenge of goats with infected Glossina morsitans morsitans. Acta tropica 37, 375–9.Google ScholarPubMed
Gray, A. R. (1965). Antigenic variation in a strain of Trypanosoma brucei transmitted by Glossina morsitans and G. palpalis. Journal of General Microbiology 41, 195–214.CrossRefGoogle Scholar
Hoare, C. A. (1972). The Trypanosomes of Mammals. Oxford: Blackwell Scientific Publications.Google Scholar
Hull, R. M., Swain, F., McCabe, W., Jones, T. W. & Clarkson, M. J. (1971). Adaptation of Trypanosoma vivax to laboratory animals. Transactions of the Royal Society of Tropical Medicine and Hygiene 65, 14–15.Google ScholarPubMed
Ketteridge, D. (1971). Studies on rodent adapted Trypanosoma vivax. Ph.D. thesis, Glasgow University.Google Scholar
Lanham, S. M. & Godfrey, D. G. (1970). Isolation of salivarian trypanosomes from man and other mammals using DEAE cellulose Experimental Parasitology 28, 521–34.CrossRefGoogle ScholarPubMed
Leeflang, P., Buys, J. & Blotkamp, , (1976). Studies on Trypanosoma vivax. Infectivity and serial maintenance of natural bovine isolates in mice. International Journal for Parasitology 6, 413–17.CrossRefGoogle ScholarPubMed
Le Ray, D., Barry, J. D., Easton, C. & Vickerman, K. (1977). First tsetse fly transmission of the ‘AnTat’ serodeme of Trypanosoma brucei. Annales de la Sociéte belge de Medecine tropicale 57, 369–81.Google ScholarPubMed
Losos, G. J. & Ikede, B. O. (1972). Review of pathology of diseases in domestic and laboratory animals caused by Trypanosoma congolense, T. vivax, T. brucei, T. rhodesiense and T. gambiense. Veterinary Pathology 9, (Suppl.), 1–71.CrossRefGoogle Scholar
Lumsden, W. H. R. (1982). Characterization and nomenclature of trypanosome serodemes and zymodemes: report of a meeting held in Edinburgh, 1978. Systematic Parasitology 4, 373–6.CrossRefGoogle Scholar
Lumsden, W. H. R., Herbert, W. J. & McNeillage, G. J. C. (1973). Techniques with Trypanosomes. Edinburgh and London: Churchill & Livingstone.Google Scholar
Moloo, S. K. (1981). Studies on the transmission of a West African stock of Trypanosoma vivax to rabbits, rats, mice and goats by Glossina morsitans morsitans and G.m. centralis. International Journal for Parasitology 11, 191–6.CrossRefGoogle Scholar
Murray, A. K. & Clarkson, M. J. (1982). Characterization of stocks of Trypanosoma vivax. II Immunological studies. Annals of tropical Medicine and Parasitology 76, 283–92.CrossRefGoogle ScholarPubMed
Pearson, T. W., Pinder, M., Roelants, G. E., Kar, S. K., Lundin, L. B., Mayor-Withey, K. S. & Hewett, R. S. (1980). Methods for derivation and detection of anti-parasite monoclonal antibodies. Journal of Immunological Methods 34, 141–54.CrossRefGoogle ScholarPubMed
Van Meirvenne, N., Janssens, P. G. & Magnus, E. (1975). Antigenic variation in syringe-passaged populations of Trypanosoma (Trypanozoon) brucei. I. Rationalization of the experimental approach. Annales de la Sociéte belge de Medecine tropicale 55, 1–23.Google ScholarPubMed
Van Meirvenne, N., Magnus, E. & Vervoort, T. (1977). Comparison of variable antigenic types produced by trypanosome strains of the subgenus Trypanozoon. Annales de la Sociéte belge de Medecine tropicale 57, 409–23.Google ScholarPubMed
Vervoort, T., Barbet, A. F., Musoke, A. J., Magnus, E., Mpimbaza, G. & van Meirvenne, N. (1981). Isotypic surface glycoproteins of trypanosomes. Immunology 44, 223–32.Google ScholarPubMed