Hostname: page-component-586b7cd67f-r5fsc Total loading time: 0 Render date: 2024-11-23T06:22:23.828Z Has data issue: false hasContentIssue false
  • English
  • Français

Changes in the messenger RNA population during sporulation of Eimeria maxima

Published online by Cambridge University Press:  06 April 2009

J. Ellis  and
T. Thurlby
J. Ellis
Affiliation:
Department of Parasitology, AFRC Institute for Animal Health, Houghton Laboratory, Houghton, Huntingdon, Cambs PE17 2DA
T. Thurlby
Affiliation:
Department of Parasitology, AFRC Institute for Animal Health, Houghton Laboratory, Houghton, Huntingdon, Cambs PE17 2DA
Get access Rights & Permissions [Opens in a new window]

Summary

Messenger RNA has been extracted from oocysts of Eimeria maxima. Using the techniques of in vitro translation and SDS–polyacrylamide gel electrophoresis, we have been able to show that major changes in gene transcription occur during sporulation. Following an overall reduction in the abundance of many mRNAs, several genes identified as the result of an increase in the abundance of their transcripts, are highly expressed during the latter stages of sporulation. A study of two genes whose transcription is down-regulated has provided evidence which shows that both single copy and repetitive sequences are regulated during sporulation of the oocyst.

Keywords

Eimeria maximasporulationmRNA abundancerepetitive sequences
Type
Research Article
Information
Parasitology , Volume 102 , Issue 1 , February 1991 , pp. 1 - 8
Copyright
Copyright © Cambridge University Press 1991

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Aviv, H. & Leder, P. (1972). Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid cellulose. Proceedings of the National Academy of Science, USA 69, 1408–12.CrossRefGoogle Scholar
Bonner, W. M. & Laskey, R. A. (1974). A film detection method for tritium labelled proteins and nucleic acids in polyacrylamide gels. European Journal of Parasitology 46, 83–8.Google Scholar
Brothers, V. M., Kuhn, I., Paul, L. S., Gabe, J. D., Andrews, W. H., Sias, S. R., McCaman, N. T., Dragon, E. A. & Files, J. G. (1988). Characterisation of a surface antigen of Eimeria tenella sporozoites and synthesis from a cloned cDNA in Escherichia coli. Molecular and Biochemical Parasitology 28, 235–48.Google Scholar
Chapman, H. D. (1978). Studies on the excystation of different species of Eimeria in vitro. Zeitschrift für Parasitenkunde 56, 115–21.Google Scholar
Chapman, H. D. (1984). Drug resistance in avian coccidia (a review). Veterinary Parasitology 15, 11–27.CrossRefGoogle ScholarPubMed
Chirgwin, J. M., Przybyla, A. E., MacDonald, R. J. & Rutter, W. J. (1979). Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18, 5294–9.Google Scholar
Chobotar, B. & Scholtyseck, E. (1982). Ultrastructure. In: The Biology of the Coccidia. (ed. Long, P. L.) pp. 145–150. Baltimore: University Park Press.Google Scholar
Clarke, L. E., Messer, L. I., Greenwood, N. M. & Wisher, M. H. (1987). Isolation of λ amp3 genomic recombinants coding for antigens of Eimeria tenella. Molecular and Biochemical Parasitology 22, 79–87.Google Scholar
Ellis, J. & Bumstead, J. (1990). Eimeria species: A study using rRNA and rDNA probes. Parasitology 101, 1–6.Google Scholar
Glisin, V., Crkvenjakov, R. & Byus, C. (1974). Ribonucleic acid isolated by cesium chloride centrifugation. Biochemistry 13, 2633–7.Google Scholar
Gubler, U. & Hoffman, B. J. (1983). A simple and very efficient method for generating cDNA libraries. Gene 25, 263–9.Google Scholar
Hanahan, D. & Meselsoln, H. (1980). Plasmid screening at high colony density. Gene 10, 63–7.Google Scholar
Hohn, B. & Collins, J. (1980). A small cosmid for efficient cloning of large DNA fragments. Gene 11, 291–8.Google Scholar
Jenkins, M. C. (1988). A cDNA encoding a merozoite surface protein of the protozoan Eimeria acervulina contains tandem-repeated sequences. Nucleic Acids Research 16, 9863.CrossRefGoogle ScholarPubMed
Jenkins, M. C. & Dame, J. B. (1987). Identification of immunodominant surface antigens of Eimeria acervulina sporozoites and merozoites. Molecular and Biochemical Parasitology 25, 155–64.Google Scholar
Jenkins, M. C., Lillehoj, H. S. & Dame, J. B. (1988). Eimeria acervulina: DNA cloning and characterisation of recombinant sporozoite and merozoite antigens. Experimental Parasitology 66, 96–107.Google Scholar
Ko, C., Cox, J., Bafundo, K., Smith, C., Stiff, M. & McDonell, M. (1989). Presence of repeated sequences in Eimeria tenella. Journal of Cell Biochemistry (Suppl.) 13E, 108.Google Scholar
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature, London 227, 680–5.Google Scholar
Lee, E. H. & Fernando, M. A. (1977). Characterisation of coccidial DNA. Zeitschrift für Parasitenkunde 53, 129–31.Google Scholar
Liberator, P. A., Hsu, J. & Turner, M. J. (1989). Tandem trinucleotide repeats throughout the nucleotide sequence of a cDNA encoding an Eimeria tenella sporozoite antigen. Nucleic Acids Research 17, 7104.Google Scholar
Long, P. L., Joyner, L. P., Millard, B. J. & Norton, C. C. (1976). A guide to laboratory techniques used in the study and diagnosis of avian coccidiosis. Folia Veterinaria Latina 6, 201–17.Google Scholar
Murray, P. K., Bhogal, B. S., Crane, M. ST. J. & McDonald, T. T. (1986). Eimeria tenella – in vivo immunisation studies with sporozoite antigen. In Research in Avian Coccidiosis, Proceedings of the Georgia Coccidiosis Conference, November 1985. (ed. McDougald, L. R., Joyner, L. P. & Long, P. L.) pp. 564–573.Google Scholar
Profus-Juchelka, H., Liberator, P. & Turner, M. (1988). Identification and characterisation of cDNA clones encoding antigens of Eimeria tenella. Molecular and Biochemical Parasitology 30, 233–42.Google Scholar
Rose, M. E. (1986). Immune response to Eimeria infections. In Research in Avian Coccidiosis, Proceedings of the Georgia Coccidiosis Conference, November 1985,Athens, Georgia, USA. (ed. McDougald, L. R., Long, P. L. & Joyner, L. P.) pp. 449–469.Google Scholar
Rose, M. E. & Hesketh, P. (1987). Eimeria tenella: effects of immunity on sporozoites within the lumen of the small intestine. Experimental Parasitology 63, 337–44.Google Scholar
Rose, M. E., Lawn, A. M. & Millard, B. J. (1984). The effect of immunity on the early events in the life-cycle of Eimeria tenella in the caecal mucosa of the chicken. Parasitology 88, 199–210.Google Scholar
St John, T. P. & Davis, R. W. (1979). Isolation of galactose-inducible DNA sequences from Saccharomyces cerevisiae by differential plaque filter hybridisation. Cell 16, 443–52.Google Scholar
Schmatz, D. M., Crane, M. ST. J. & Murray, P. K. (1984). Purification of Eimeria sporozoites by DE-52 anion exchange chromatography. Journal of Protozoology 31, 181–3.Google Scholar
Sutton, C. A., Shirley, M. W. & Wisher, M. H. (1989). Characterization of coccidial proteins by two-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Parasitology 99, 175–82.Google Scholar
Wallach, M. G., Mencher, D., Yarus, S., Pillemer, G., Halabi, A. & Pugatsch, T. (1989). Eimeria maxima: identification of gametocyte protein antigens. Experimental Parasitology 68, 49–56.Google Scholar
Wang, C. C. & Stotish, R. L. (1975). Changes of nucleic acids and proteins in the oocysts of Eimeria tenella during sporulation. Journal of Protozoology 22, 438–43.Google Scholar
Wisher, M. H. (1986). Identification of the sporozoite antigens of Eimeria tenella. Molecular and Biochemical Parasitology 21, 7–15.CrossRefGoogle ScholarPubMed