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Characterization of a high molecular weight protective antigen of Plasmodium yoelli

Published online by Cambridge University Press:  06 April 2009

A. A. Holder  and
R. R. Freeman
A. A. Holder
Affiliation:
Department of Molecular Biology, The Wellcome Research Laboratories, Langley Court, Beckenham, Kent BR3 3BS
R. R. Freeman
Affiliation:
Department of Molecular Biology, The Wellcome Research Laboratories, Langley Court, Beckenham, Kent BR3 3BS
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Summary

A 230000 molecular weight Plasmodium yoelii protective antigen was characterized. Two monoclonal antibodies against P. yoelii immunoprecipitated the 230000 mol. wt protein and a number of lower molecular weight polypeptides. These polypeptides were shown by peptide mapping and specific antibody binding to be fragments of the large protein. Iodination experiments suggested that the lower molecular weight species may be present on the surface of the merozoite. The protein was found not to be glycosylated. By serology, related antigens were shown to be associated with blood-stage schizonts of P. vinckei subspp., P. chabaudi subspp. and P. falciparum.

Type
Research Article
Information
Parasitology , Volume 88 , Issue 2 , April 1984 , pp. 211 - 219
Copyright
Copyright © Cambridge University Press 1984

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