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Characterization of buffalo-derived theilerial parasites with monoclonal antibodies and DNA probes

Published online by Cambridge University Press:  06 April 2009

P. A. Conrad ,
O. K. Ole-Moiyoi ,
C. L. Baldwin ,
T. T. Dolan ,
C. J. O'Callaghan ,
R. E. G. Njamunggehr ,
J. G. Grootenhuis ,
D. A. Stagg ,
B. L. Leitch  and
A. S. Young
P. A. Conrad
Affiliation:
1International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya
O. K. Ole-Moiyoi
Affiliation:
1International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya
C. L. Baldwin
Affiliation:
1International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya
T. T. Dolan
Affiliation:
1International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya
C. J. O'Callaghan
Affiliation:
1International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya
R. E. G. Njamunggehr
Affiliation:
1International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya
J. G. Grootenhuis
Affiliation:
2Wildlife Diseases Research Project, Veterinary Research Laboratory, P.O. Kabete, Kenya
D. A. Stagg
Affiliation:
3National Veterinary Research Centre Muguga, Kenya Agricultural Research Institute, P.O. Box 32, Kikuyu, Kenya
B. L. Leitch
Affiliation:
3National Veterinary Research Centre Muguga, Kenya Agricultural Research Institute, P.O. Box 32, Kikuyu, Kenya
A. S. Young
Affiliation:
3National Veterinary Research Centre Muguga, Kenya Agricultural Research Institute, P.O. Box 32, Kikuyu, Kenya
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Summary

The characteristics of intra-lymphocytic Theileria isolated from African buffalo and from cattle that were infected with buffalo-derived parasites were evaluated using anti-schizont monoclonal antibodies (mAbs) and DNA probes. Antigenic differences were revealed by the reactivities of 27 mAbs with the buffalo-derived parasites isolated from different animals. Antigenic diversity was also seen with Theileria-infected lymphoblastoid cell isolates taken from the lymph nodes and blood of the same animals. Two DNA probes, selected from a genomic library of T. parva piroplasm DNA cloned in λgt11, showed specific hybridization to parasite DNA in Southern blots of restriction enzyme-digested, lymphoblastoid cells infected with buffalo-derived theilerial parasites. Genotypic differences between the buffalo-derived parasites were revealed by the restriction fragment length polymorphisms seen with hybridization of those probes to DNA from cloned and uncloned Theileria-infected cell lines. The evaluation of theilerial parasites derived from buffalo and from cattle which underwent typical T. p. lawrencei reactions, after being infected with buffalo-derived theilerial parasites, did not show any specific phenotypic or genotypic characteristics of these parasites that would distinguish them from T. p. parva and T. p. bovis parasites. The validity of these subspecies distinctions is discussed.

Keywords

Theileria parvaDNA probesbuffalo characterizationmonoclonal antibodies
Type
Research Article
Information
Parasitology , Volume 98 , Issue 2 , April 1989 , pp. 179 - 188
Copyright
Copyright © Cambridge University Press 1989

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References

Refernces

Brown, C. G. D. (1983). Theileria. In In Vitro Cultivationof Protozoan Parasites (ed. Jensen, J. B.), pp. 243–84.Boca Raton, Florida: CRC Pres, Inc.Google Scholar
Brown, W. C. & Grab, D. J. (1985). Biological and biochemical characterization of bovine interleukin 2. Studies with cloned bovine T cells. Journal of Immunology 133, 3184–9.CrossRefGoogle Scholar
Conrad, P. A., Iams, K., Brown, W. C., Sohanpal, B. & Ole-Moiyoi, O. K. (1987 b). DNA probes detect genomic diversity in Theileria parva stocks. Molecular and Biochemical Parasitology 25, 213–26.CrossRefGoogle ScholarPubMed
Conrad, P. A., Stagg, D. A., Grootenhuis, J. G., Irvin, A. D., Newson, J., Njamunggeh, R. E. G., Rossiter, P. B. & Young, A. S. (1987 a). Isolation of Theileria parasites from African buffalo (Syncerus caffer) and characterization with anti-schizont monoclonal antibodies. Parasitology 94, 413–23.CrossRefGoogle ScholarPubMed
Dobbelaere, D. A. E., Spooner, P. R., Barry, W. C., Irvin, A. D. (1984). Monoclonal antibody neutralizes the sporozoite stage of different Theileria parva stocks. Parasite Immunology 6, 361–70.CrossRefGoogle ScholarPubMed
Grootenhuis, J. G., Leitch, B. L., Stagg, D. A., Dolan, T. T. & Young, A. S. (1987). Experimental induction of Theileria parva lawrencei carrier state in an African buffalo (Syncerus caffer). Parasitology 94, 425–31.CrossRefGoogle Scholar
Irvin, A. D. (1987). Characterization of species and strains of Theileria. Advances in Parasitology 26, 145–97.CrossRefGoogle ScholarPubMed
Kearney, J. F., Radbruch, A., Leisegang, B. & Rajewsky, K. (1979). A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines. Journal of Immunology 123, 1548–50.CrossRefGoogle ScholarPubMed
koch, R. (1905). Vorläufige Mitteilungen über die Ergebnisse einer Forschungsreise nach Ostafrika. Deutsches Medizinisches Wochenschrift 47, 1865–9.CrossRefGoogle Scholar
koch, R. (1906). Beiträge zur Entwicklungsgeshichte der Piroplasmen. Zeitschrift für Hygiene und Infektionskrankheiten 54, 1–9.CrossRefGoogle Scholar
koch, H. T., Ocama, J. G. R., Munatswa, F. C., Byrom, B., Norval, R. A. I., Spooner, P. R., Conrad, P. A. & Irvin, A. D. (1988). Isolation and characterization of bovine Theileria parasites in Zimbabwe. Veterinary Parasitology 28, 19–31.CrossRefGoogle ScholarPubMed
Lawrence, J. A. & Mackenzie, P. K. I. (1980). Isolation of a non-pathogenic Theileria of cattle transmitted by Rhipicephalus appendiculatus. Zimbabwe Veterinary Journal 11, 27–36.Google Scholar
Maniatis, T., Fritsch, E. F. & Sambrook, J. (1982).Molecular Cloning, a Laboratory Manual. New York: Cold Spring Harbor Laboratory, Cold Spring Harbor.Google Scholar
Minami, T., Spooner, P. R., Irvin, A. D., Ocama, J. G. R., Dobbelaere, D. A. E. & Fujinaga, T. (1983). Characterization of stocks of Theileria parva by monoclonal antibody profiles. Research in Veterinary Science 35, 334–40.CrossRefGoogle ScholarPubMed
Neitz, W. O., Canham, A. S. & Kluge, E. B. (1955). Corridor Disease: a fatal form of bovine Theileriosis encountered in Zululand. Journal of the South African Veterinary Medical Association 26, 79–87.Google Scholar
Pearson, T. W., Pinder, M., Roelants, G. E., Kar, S. K., Lundin, L. B., Mayor-Withey, K. S. & Hewett, R. S.(1980). Methods for derivation and detection of antiparasite monoclonal antibodies. Journal of Immunological Methods 34, 141–54.CrossRefGoogle ScholarPubMed
Schein, E., Warnecke, M. & Kirmse, P. (1977). Development of Theileria parva (Theiler, 1904) in the gut of Rhipicephalus appendiculatus (Neumann, 1901). Parasitology 75, 309–16.CrossRefGoogle ScholarPubMed
Shiels, B., McDougall, C., Tait, A. & Brown, C. G. D.(1986). Antigenic diversity of Theileria annulata macroschizonts. Veterinary Parasitology 21, 1–10.CrossRefGoogle ScholarPubMed
Stagg, D. A., Conrad, P. A. & Rossiter, P. B. (1987). Isolation of Theileria parva lawrencei-infected lymphoid cell lines from free-ranging African buffaloes (Syncerus caffer). Research in Veterinary Science 43, 124–6.CrossRefGoogle ScholarPubMed
Stagg, D. A., Young, A. S., Leitch, B. L., Grootenhuis, J. G. & Dolan, T. T. (1983). Infection of mammalian cells with Theileria species. Parasitology 86, 243–54.CrossRefGoogle ScholarPubMed
Uilenberg, G. (1981). Theilerial species of domestic livestock. In Advances in the Control of Theileriosis (ed. Irvin, A. D., Cunningham, M. P. and Young, A. S.), pp. 4–37. London: Martinus Nijhoff.CrossRefGoogle Scholar
Young, A. S., Brown, C. G. D., Burridge, M. J., Grootenhuis, J. G., Kanhai, G. K., Purnell, R. E. & Stagg, D. A. (1978). The incidence of theilerial parasites in East African buffalo (Syncerus Caffer). Tropenmedizin und Parasitologie 29, 281–9.Google ScholarPubMed
Young, A. S., Radley, D. E., Cunningham, M. P., Musisi, F. L., Payne, R. C. & Purnell, R. E. (1977). Exposure of immunized cattle to prolonged natural challenge of Theileria lawrencei derived from African buffalo (Syncerus caffer). Veterinary Parasitology 3, 283–90.CrossRefGoogle Scholar