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Detection of enteric parasites and molecular characterization of Giardia duodenalis and Blastocystis sp. in patients admitted to hospital in Ankara, Turkey

Published online by Cambridge University Press:  28 September 2020

Fakhriddin Sarzhanov Open the ORCID record for Fakhriddin Sarzhanov [Opens in a new window] ,
Pamela Carolina Köster ,
Funda Dogruman-Al ,
Begoña Bailo ,
Alejandro Dashti ,
Filiz Demirel-Kaya  and
David Carmena Open the ORCID record for David Carmena [Opens in a new window]
Fakhriddin Sarzhanov
Affiliation:
Department of Medical Microbiology, School of Medicine, Gazi University, 06490Ankara, Turkey Faculty of Medicine, Akhmet Yassawi International Kazakh-Turkish University, 161200Turkestan, Kazakhstan
Pamela Carolina Köster
Affiliation:
Parasitology Reference and Research Laboratory, National Centre for Microbiology, Health Institute Carlos III, Ctra. Majadahonda-Pozuelo Km 2, 28220 Majadahonda, Madrid, Spain
Funda Dogruman-Al*
Affiliation:
Section of Medical Parasitology, Department of Medical Microbiology, School of Medicine, Gazi University, 06490Ankara, Turkey
Begoña Bailo
Affiliation:
Parasitology Reference and Research Laboratory, National Centre for Microbiology, Health Institute Carlos III, Ctra. Majadahonda-Pozuelo Km 2, 28220 Majadahonda, Madrid, Spain
Alejandro Dashti
Affiliation:
Parasitology Reference and Research Laboratory, National Centre for Microbiology, Health Institute Carlos III, Ctra. Majadahonda-Pozuelo Km 2, 28220 Majadahonda, Madrid, Spain
Filiz Demirel-Kaya
Affiliation:
Medical Microbiology Laboratory, Ankara Education and Research Hospital, Health Science University, 06230Ankara, Turkey
David Carmena*
Affiliation:
Parasitology Reference and Research Laboratory, National Centre for Microbiology, Health Institute Carlos III, Ctra. Majadahonda-Pozuelo Km 2, 28220 Majadahonda, Madrid, Spain
*
Author for correspondence: David Carmena, E-mail: dacarmena@isciii.es, Funda Dogruman-Al, E-mail: alfunda@yahoo.com
Author for correspondence: David Carmena, E-mail: dacarmena@isciii.es, Funda Dogruman-Al, E-mail: alfunda@yahoo.com
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Abstract

This epidemiological study assesses the occurrence of enteric parasites in 4303 patients attended at two public hospitals in Ankara (Turkey) during 2018–2019. Microscopy was used as a screening test. Giardia duodenalis was also identified using a commercial ELISA for the detection of parasite-specific coproantigens. Giardia-positive samples by microscopy/ELISA were confirmed by real-time PCR and characterized using a multilocus genotyping scheme. Blastocystis sp. was genotyped in a sample subset. Blastocystis sp. (11.1%, 95% CI 11.4‒14.8%) and G. duodenalis (1.56%, 95% CI 1.22‒1.96) were the most prevalent pathogens found. Cryptosporidium spp., Entamoeba histolytica and intestinal helminths were only sporadically (<0.5%) found. For G. duodenalis, sequence (n = 30) analyses revealed the presence of sub-assemblages AII (23.3%), discordant AII/AIII (23.3%) and mixed AII + AIII (6.7%) within assemblage A, and BIII (10.0%), BIV (3.3%) and discordant BIII/BIV (23.3%) within assemblage B. Two additional sequences (6.7%) were assigned to the latter assemblage but sub-assemblage information was unknown. No associations between G. duodenalis assemblages/sub-assemblages and sociodemographic and clinical variables could be demonstrated. For Blastocystis sp., sequence (n = 6) analyses identified subtypes ST1, ST2 and ST3 at equal proportions. This is the first molecular characterization of G. duodenalis based on MLG conducted in Turkey to date.

Keywords

BlastocystisCryptosporidiumDientamoeba fragilisEntamoeba histolyticaGiardia duodenalisintestinal parasitesmolecular epidemiologymultilocus genotypingTurkey
Type
Research Article
Information
Parasitology , Volume 148 , Issue 5 , April 2021 , pp. 550 - 561
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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