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Distinct courses of infection with Leishmania (L.) amazonensis are observed in BALB/c, BALB/c nude and C57BL/6 mice

Published online by Cambridge University Press:  19 February 2016

LEONARDO G. VELASQUEZ ,
MARIANA K. GALUPPO ,
ELOIZA DE REZENDE ,
WESLEY N. BRANDÃO ,
JEAN PIERRE PERON ,
SILVIA R. B. ULIANA ,
MARIA IRMA DUARTE  and
BEATRIZ S. STOLF
LEONARDO G. VELASQUEZ
Affiliation:
Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil
MARIANA K. GALUPPO
Affiliation:
Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil
ELOIZA DE REZENDE
Affiliation:
Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil
WESLEY N. BRANDÃO
Affiliation:
Department of Immunology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil
JEAN PIERRE PERON
Affiliation:
Department of Immunology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil
SILVIA R. B. ULIANA
Affiliation:
Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil
MARIA IRMA DUARTE
Affiliation:
Department of Pathology, University of São Paulo School of Medicine, São Paulo, Brazil
BEATRIZ S. STOLF*
Affiliation:
Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil
*
*Corresponding author: Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil. E-mail: bstolf@usp.br
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Summary

Leishmania (L.) amazonensis [L. (L.) amazonensis] is widely distributed in Brazil and its symptomatic infections usually lead to few localized lesions and sometimes to diffuse cutaneous form, with nodules throughout the body, anergy to parasite antigens and poor therapeutic response. The variability of these manifestations draws attention to the need for studies on the pathophysiology of infection by this species. In this study, we analysed the course and immunological aspects of L. (L.) amazonensis infection in BALB/c and C57BL/6 strains, both susceptible, but displaying different clinical courses, and athymic BALB/c nude, to illustrate the role of T cell dependent responses. We analysed footpad thickness and parasite burden by in vivo imaging. Furthermore, we evaluated the cellular profile and cytokine production in lymph nodes and the inflammatory infiltrates of lesions. Nude mice showed delayed lesion development and less inflammatory cells in lesions, but higher parasite burden than BALB/c and C57BL/6. BALB/c and C57BL/6 mice had similar parasite burdens, lesion sizes and infiltrates until 6 weeks after infection, and after that C57BL/6 mice controlled the infection. Small differences in parasite numbers were observed in C57BL/6 macrophages in vitro, indicating that in vivo milieu accounts for most differences in infection. We believe our results shed light on the role of host immune system in the course of L. (L.) amazonensis infection by comparing three mouse strains that differ in parasitaemia and inflammatory cells.

Keywords

Leishmania (L.) amazonensisinfection courseparasite burdenin vivo imagingmouse strainsnude miceimmune responseinflammation
Type
Research Article
Information
Parasitology , Volume 143 , Issue 6 , May 2016 , pp. 692 - 703
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Copyright
Copyright © Cambridge University Press 2016 

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