Hostname: page-component-78c5997874-s2hrs Total loading time: 0 Render date: 2024-11-16T05:21:09.674Z Has data issue: false hasContentIssue false
  • English
  • Français

The effect of human immune IgG on the in vitro development of Plasmodium falciparum

Published online by Cambridge University Press:  06 April 2009

G. H. Mitchell ,
G. A. Butcher ,
A. Voller  and
S. Cohen
G. H. Mitchell
Affiliation:
Department of Chemical Pathology, Guy's Hospital Medical School, London, SE1 9RT, and Department of Clinical Tropical Medicine, London School of Hygiene and Tropical Medicine
G. A. Butcher
Affiliation:
Department of Chemical Pathology, Guy's Hospital Medical School, London, SE1 9RT, and Department of Clinical Tropical Medicine, London School of Hygiene and Tropical Medicine
A. Voller
Affiliation:
Nuffield Institute of Comparative Medicine, Zoological Society of London, Regent's Park, London, N.W.I, and Department of Clinical Tropical Medicine, London School of Hygiene and Tropical Medicine
S. Cohen
Affiliation:
Department of Chemical Pathology, Guy's Hospital Medical School, London, SE1 9RT, and Department of Clinical Tropical Medicine, London School of Hygiene and Tropical Medicine
Get access Rights & Permissions [Opens in a new window]

Extract

Plasmodium falciparum parasites infecting Aotus trivirgatus erythrocytes were cultured in media (Harvard and TC199) augmented with human, foetal calf, or other sera. Conditions were established which supported growth of parasites and allowed cyclical multiplication when fresh erythrocytes (from Aotus or Homo) were added in sub-culture (mean multiplication rate: x 3). Immunoglobulin G pools, prepared from plasma collected in endemic malarious areas in Africa and from unexposed Britons, were tested for effects on the in vitro growth (measured by incorporation of tritiated leucine) and multiplication of parasites. Whilst non-immune IgG was without effect, IgG from both East and West Africa inhibited the multiplication of East African (Uganda-Palo Alto strain) parasites.

Type
Research Article
Information
Parasitology , Volume 72 , Issue 2 , April 1976 , pp. 149 - 162
Copyright
Copyright © Cambridge University Press 1976

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Anfinsen, C. B., Geiman, Q. M., McKee, R. W., Ormsbee, R. A. & Ball, E. G. (1946). Factors affecting the growth of Plasmodium knowlesi in vitro. Journal of Experimental Medicine 84, 607–21.CrossRefGoogle ScholarPubMed
Bass, G. C. & Johns, F. M. (1912). The cultivation of malaria plasmodia in vitro. Journal of Experimental Medicine 16, 567–79.CrossRefGoogle Scholar
Brown, K. N. & Brown, I. N. (1965). Immunity to malaria: antigenic variation in chronic infections of Plasmodium knowlesi. Nature, London 208, 1286–8.CrossRefGoogle ScholarPubMed
Butcher, G. A. & Cohen, S. (1971). Short term culture of Plasmodium knowlesi. Parasitology 62, 309–20.CrossRefGoogle ScholarPubMed
Butcher, G. A. & Cohen, S. (1972). Antigenic variation and protective immunity in Plasmodium knowlesi malaria. Immunology 23, 503–21.Google ScholarPubMed
Cohen, S. & Butcher, G. A. (1970). Properties of protective malarial antibody. Immunology 19, 369–83.Google ScholarPubMed
Cohen, S., Butcher, G. A. & Crandall, R. B. (1969). Action of malarial antibody in vitro. Nature, London 223, 368–71.Google Scholar
Cohen, S., McGregor, I. A. & Carrington, S. P. (1961). Gamma globulin and acquired immunity to human malaria. Nature, London 192, 733–7.Google Scholar
Diggs, C. L., Pavanand, K., Permpanich, B., Numsuwankijkul, V., Haupt, R. & Chuanak, N. (1971). Penetration of human foetal erythrocytes by Plasmodium falciparum. Journal of Parasitology 57, 187–8.Google Scholar
Diggs, C. L., Wellde, B. T., Anderson, J. S., Weber, R. M. & Rodriguez, E. (1972). The protective effect of African human immunoglobulin G in Aotus trivirgatus infected with Asian Plasmodium falciparum. Proceedings of the Helminthological Society of Washington 39 (special issue: Basic research in malaria), 449–56.Google Scholar
Eagle, H. (1971). Buffer combinations for mammalian cell culture. Science (New York) 174, 500–3.CrossRefGoogle ScholarPubMed
Geiman, Q. M. & Meagher, M. J. (1967). Susceptibility of a new world monkey to Plasmodium falciparum from man. Nature, London 215, 437–9.Google Scholar
Geiman, Q. M., Siddiqui, W. A. & Schnell, J. V. (1966). In vitro studies on erythrocytic stages of plasmodia. Military Medicine 131 (Supplement) 1015–25.CrossRefGoogle ScholarPubMed
Goodwin, H. H. & Holloway, C. W. (1972). Red Data Book 1. (Mammalia). International Union for the Conservation of Nature. Morges: Switzerland.Google Scholar
Jeffery, G. M. (1962). Survival of trophozoites of Plasmodium berghei and Plasmodium gallinaceum in glycerolised whole blood at low temperatures. Journal of Parasitology 48, 601–6.Google Scholar
McGregor, I. A., Carrington, S. P. & Cohen, S. (1963). Treatment of East African P. falciparum malaria with West African human γ-globulin. Transactions of the Royal Society of Tropical Medicine and Hygiene 57, 170–5.Google Scholar
Mitchell, G. H., Butcher, G. A. & Cohen, S. (1975). Merozoite vaccination against Plasmodium knowlesi malaria. Immunology 29, 397–407.Google ScholarPubMed
Morgan, J. F., Morton, H. J. & Parker, R. C. (1950). Nutrition of animal cells in tissue culture: I. Initial studies on a synthetic medium. Proceedings of the Society for Experimental Biology and Medicine 73, 1–8.Google Scholar
Phillips, R. S., Trigg, P. I., Scott-Finnigan, T. J. & Bartholomew, R. K. (1972). Culture of Plasmodium falciparum in vitro: a sub-culture technique used for demonstrating anti-plasmodial activity in serum from some Gambians, resident in an endemic malarious area. Parasitology 65, 525–35.CrossRefGoogle Scholar
Sadun, E. H., Hickman, R. L., Wellde, B. T., Moon, A. P. & Udeozo, I. O. K. (1966). Active and passive immunisation of chimpanzees infected with West African and South East Asian strains of Plasmodium falciparum. Military Medicine 131 (Supplement), 1250–62.CrossRefGoogle Scholar
Siddiqui, W. A. & Schnell, J. V. (1973). Use of various buffers for in vitro cultivation of malarial parasites. Journal of Parasitology 59, 516–19.Google Scholar
Trager, W. (1966). Co-enzyme A and the anti-malarial action in vitro of anti-pantothenate against P. lophurae, P. coatneyi and P. falciparum. Transactions of the New York Academy of Science, Series I 28, 1094–108.Google Scholar
Trager, W. (1971). A new method for intraerythrocytic cultivation of malaria parasites (Plasmodium coatneyi and Plasmodium falciparum). Journal of Protozoology 18, 392–9.Google Scholar
Trigg, P. I. (1967). In vitro growth of Plasmodium knowlesi and Plasmodium falciparum. Nature, London 213, 1019–20.Google Scholar
Voller, A. (1971). Variant specific schizont agglutination antibodies in human malaria. (Plasmodium falciparum) infections in Aotus. Transactions of the Royal Society of Tropical Medicine and Hygiene 65, 2–3.Google ScholarPubMed
Voller, A. & Richards, W. H. G. (1970). Immunity to Plasmodium falciparum in owl monkeys (Aotus trivirgatus). Zeitschrift für Tropenmedizin und Parasitologic 21, 159–66.Google ScholarPubMed
Voller, A., Green, D. I. & Richards, W. H. G. (1973). Cross immunity studies with East and West African strains of Plasmodium falciparum in owl monkeys (Aotus trivirgatus). Journal of Tropical Medicine and Hygiene 76, 135–9.Google Scholar
Voller, A., Richards, W. H. G., Hawkey, C. M. & Ridley, D. S. (1969). Human malaria (Plasmodium falciparum) in owl monkeys (Aotus trivirgatus). Journal of Tropical Medicine and Hygiene 72, 153–60.Google Scholar