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Effect of parasitism on plasma sex-specific proteins in Cyphocarax gilbert (Teleost, Curimatidae)

Published online by Cambridge University Press:  03 February 2005

L. GOMES DA SILVA ,
J. S. AZEVEDO ,
M. A. SILVA-NETO ,
N. R. WILLE LIMA  and
M. DANSA-PETRETSKI
L. GOMES DA SILVA
Affiliation:
Laboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, Rio de Janeiro, Brazil
J. S. AZEVEDO
Affiliation:
Laboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, Rio de Janeiro, Brazil
M. A. SILVA-NETO
Affiliation:
Departamento de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
N. R. WILLE LIMA
Affiliation:
Departamento de Biologia Geral, Universidade Federal Fluminense, Rio de Janeiro, Brazil
M. DANSA-PETRETSKI
Affiliation:
Laboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, Rio de Janeiro, Brazil
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Abstract

Cyphocarax gilbert (Szidat, L., 1948) is a fish commonly found in coastal drainage of eastern Brazil. This fish is sometimes caught with signs of infection by the crustacean Riggia paranensis, a haematophagous parasite. A remarkable feature of infected fish is that they lack gonads. In this paper we have analysed the frequency of parasitism, the gonadal development of non-infected fish and the profile of plasma proteins in both infected and non-infected specimens. Two reproductive periods/year were observed, beginning in February and August. On average, 40% of fish were infected, in the Itabapoana River (Brazil). Sex-specific proteins were identified by electrophoresis. SDS-PAGE analysis demonstrated that a 143 kDa female-specific glycolipoprotein (FSP) is a calcium-binding phosphoprotein. FSP was isolated through ultracentrifugation and SDS-PAGE analysis showed that the native protein is composed of three polypeptides of 143, 100 and 70 kDa. Both FSP and a 33 kDa male-specific protein (MSP) are absent from infected fish plasma. FSP levels in female plasma changes with the developmental stage of gonads. Altogether these data suggest that the FSP corresponds to fish vitellogenin. Furthermore, the absence of the above-mentioned proteins in infected fish suggests that R. paranensis might interfere with the regular hormonal process of fish vitellogenesis.

Keywords

crustaceanparasitic castratorplasma proteinsreproductionvitellogeninCyphocarax gilbertRiggia paranensis
Type
Research Article
Information
Parasitology , Volume 130 , Issue 6 , June 2005 , pp. 653 - 659
Copyright
© 2005 Cambridge University Press

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