Hostname: page-component-586b7cd67f-l7hp2 Total loading time: 0 Render date: 2024-11-23T02:32:39.889Z Has data issue: false hasContentIssue false
  • English
  • Français

Effects of three anthelmintics on the tegument of Hymenolepis fraterna (Cestoda)

Published online by Cambridge University Press:  06 April 2009

S. R. Stoitsova ,
L. N. Gorchilova  and
J. Daněk
S. R. Stoitsova
Affiliation:
Institute of Parasitology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 25, 1113 Sofia, Bulgaria
L. N. Gorchilova
Affiliation:
Institute of Parasitology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 25, 1113 Sofia, Bulgaria
J. Daněk
Affiliation:
Center for Veterinary Drugs, Research Institute for Feed Supplements and Veterinary Drugs, Pohoří-Chotouň, 254 49 Jilové near Prague, Czechoslovakia
Get access Rights & Permissions [Opens in a new window]

Summary

The in vivo effects of the anthelmintics taenifugin, VÚFB 14170 and VÚFB 15269 on the tegument of Hymenolepis fraterna have been examined by SEM, TEM and cytochemistry. The drugs were given to H. fraterna-infected mice on the 14th day post-infection in a single oral dose of 150, 200 and 200 mg/kg, respectively. By 72 h post-treatment, the drug-induced pathomorphological changes in the tegument indicated that all three drugs had a significant effect. Changes were most pronounced on the brush border and in the intercellular material. On the apical surface, there was blebbing as well as accumulation of membrane fragments over the microthrix tips and erosion of the brush border. The intercellular material was changed in structure, showing increased electron density in some areas and oedema of the intercellular spaces in other areas. There were also fractures of the tegument of variable depth, sometimes reaching to the parenchyma. These results suggest altered tegumental integrity and, occasionally, complete disruption of the selective permeability barrier created by the normal tegument. This suggestion is further supported by the penetration of ruthenium red into some tegumental areas and its distribution into the intercellular spaces, down to the parenchyma. The intrategumental lysosomes also appeared to be significantly activated. There was evidence of autophagy in both distal cytoplasm and tegumental cells. Mature and gravid proglottides were more susceptible to drug damage than those in the anterior strobila and neck.

Keywords

Hymenolepis fraternaanthelminticstegumental ultrastructure
Type
Research Article
Information
Parasitology , Volume 104 , Issue 1 , February 1992 , pp. 143 - 152
Copyright
Copyright © Cambridge University Press 1992

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Andreassen, J. & Hoole, D. (1989). Repair of Hymenolepis diminuta after complement-mediated damage. Parasitology 99, 437–43.CrossRefGoogle ScholarPubMed
Becker, B., Mehlhorn, H., Andrews, P. & Thomas, H. (1981). Ultrastructural investigations on the effect of praziquantel on the tegument of five species of cestodes. Zeitschrift für Parasitenkunde 64, 257–69.CrossRefGoogle ScholarPubMed
Conder, G. A., Marchiondo, A. A., Williams, J. F. & Anderson, F. L. (1983). Ultrastructural characterization of serum-induced changes in the tegument of Taenia taeniaeformis. Journal of Parasitology 69, 838–45.CrossRefGoogle ScholarPubMed
Conradt, U. & Peters, W. (1989). Investigations on the occurrence of pinocytosis in the tegument of Schistocephalus solidus. Parasitology Research 75, 630–5.CrossRefGoogle Scholar
Ericsson, J. L. (1969). Mechanisms of cellular autophagy. In Lysosomes in Biology and Pathology, Vol. 2 (ed. Dingle, J. T. & Fell, H. B.), pp. 345–94. Amsterdam, London: North Holland Publishing Co.Google Scholar
Fairweather, I., Anderson, H. R. & Baldwin, T. M. A. (1987). Fasciola hepatica: Tegumental surface alterations following treatment in vitro with the deacetylated (amine) metabolite of diamphenethide. Parasitology Research 73, 99–106.CrossRefGoogle ScholarPubMed
Fairweather, I., Anderson, H. R. & Threadgold, L. T. (1986). Fasciola hepatica: Tegumental changes induced in vitro by the deacetylated (amine) metabolite of diamphenethide. Experimental Parasitology 62, 336–48.CrossRefGoogle ScholarPubMed
Gorchilova, L. N., Poljakova-Krusteva, O. T., Stoitsova, S. R. & DanĚk, J. (1991). Morphofunctional changes in the tegument and the intestinal wall of adult Fasciola hepatica under the action of Oxyclosanide and VUFB 15269. Helminthologia 28, 67–74.Google Scholar
Gorchilova, L. N., Spaldonova, R. & Poljakova-Krusteva, O. T. (1988). Morphofunctional characteristics of the tegument and the gut wall of mature Fasciola hepatica after fenbendazole/triclabendazole treatment. Helminthologia 25, 147–55.Google Scholar
Hamajima, F., Fujino, T., Yamagami, K. & Eriguchi, N. (1979). Studies on the in vitro effects of bithionol and meniclopholan on flukes of Clonorchis sinensis, Metagonimus takahashii and Paragonimus miyazakii. International Journal for Parasitology 9, 241–9.CrossRefGoogle ScholarPubMed
Hart, R. J., Turner, R. & Wilson, R. G. (1977). A biochemical and ultrastructural study of the mode of action of bunamidine against Hymenolepis nana. International Journal for Parasitology 7, 129–34.CrossRefGoogle ScholarPubMed
Hipkiss, J. B., Skinner, A. & Branford, White, C., J. (1987). Biochemical and ultrastructural investigation on the effect of Stelazine (trifluoroperazine) on Hymenolepis diminuta (Cestoda). Parasitology 94, 135–49.CrossRefGoogle ScholarPubMed
Holy, J. M. & Oaks, J. A. (1987). Mechanical integration of muscle, tegument, and subtegumental tissues by anchoring microfibrils in the cestode Hymenolepis diminuta. Tissue and Cell 19, 881–91.CrossRefGoogle ScholarPubMed
Hustead, S. T. & Williams, J. F. (1977). Permeability studies on taeniid metacestodes. II. Antibody-mediated effects on membrane permeability in larvae of Taenia crassiceps. Journal of Parasitology 63, 322–6.CrossRefGoogle ScholarPubMed
Krustev, L. (1982). Lysosomes. In Ultrastructural Basis of Pathology (ed. Krustev, L.). pp. 128–163. Sofia: Medizina i Fizkultura.Google Scholar
Lindroos, P. (1984). Observations on the extracellular spaces and intracellular junctions in Diphyllobothrium dendriticum (Cestoda). Acta Zoologica 68, 147–51.CrossRefGoogle Scholar
Lindroos, P. & Wikgren, M. (1987). Extracellular matrix in platyhelminths, with special reference to the presence of fibronectin. Acta Zoologica 68, 147–51.CrossRefGoogle Scholar
Luft, J. H. (1971). Ruthenium red and violet. I. Chemistry purification, methods of use for electron microscopy and mechanisms of action. Anatomical Record 171, 347–68.CrossRefGoogle Scholar
Lumsden, R. D. & Hildreth, M. B. (1983). The fine structure of adult tapeworms. In Biology of the Eucestoda, Vol. 1 (ed. Arme, C. & Pappas, P). pp. 177–234, London: Academic Press.Google Scholar
Oaks, J. A. & Mueller, J. F. (1981). Location of carbohydrate in the tegument of the plerocercoid of Spirometra mansonoides. International Journal for Parasitology 67, 325–31.CrossRefGoogle Scholar
Pappas, P. W. (1988). Acid phosphatase activity in the isolated brush border membrane of the tapeworm, Hymenolepis diminuta: Partial characterization and differentiation from the alkaline phosphatase activity. Journal of Cellular Biochemistry 37, 395–403.CrossRefGoogle ScholarPubMed
Poljakova-Krusteva, O. T., Gorchilova, L. N., Mizinska-Boevska, YA. D. & Stoitsova, S. R. (1985). Lysosomes and lysosome-like structures in the teguments of trematodes and cestodes. Khelmintologiya 20, 66–79.Google Scholar
Poljakova-Krusteva, O. T., Mizinska-Boevska, YA. D. & Stoitsova, S. R. (1983). A cytochemical study of some phosphatases in the teguments of two cestode species. Khelmintologiya 16, 64–7.Google Scholar
Poljakova-Krusteva, O. T., Stoitsova, S. R. & Mizinska-Boevska, YA. D. (1984). Pinocytosis in the tegument of Hymenolepis fraterna. Khelmintologiya 17, 52–7.Google Scholar
Rew, R. S., Fetterer, R. H. & Martin, T. C. (1983). Fasciola hepatica: effects of diamphenethide free amine on in vitro physiology, biochemistry and morphology. Experimental Parasitology 55, 159–67.CrossRefGoogle ScholarPubMed
Robinson, R. D., Andreassen, J. & Arme, C. (1987). Hymenolepis diminuta: Ultrastructural observations on complement-mediated tegumental lysis and destrobilation of 4-day-old worms in vitro. International Journal for Parasitology 17, 1225–32.CrossRefGoogle ScholarPubMed
Rogan, M. T. & Richards, K. S. (1986). Echinococcus granulosus: in vitro effects of monensin on the tegument of the protoscolex. Parasitology 93, 347–55.CrossRefGoogle ScholarPubMed
Schmidt, J. (1988). Expression of glycoconjugates on normally developing and immunologically impaired Hymenolepsis diminuta. Parasitology Research 75, 155–61.CrossRefGoogle Scholar
Simpson, A. J. G. & Mclaren, D. J. (1982). Schistosoma mansoni: tegumental damage as a consequence of lectin binding. Experimental Parasitology 53, 105–16.CrossRefGoogle ScholarPubMed
Stoitsova, S. R. (1983). Structural and functional characteristics of membrane structures in the tegument of Hymenolepis fraterna (Stiles, 1906). Ph. D. thesis, Central Laboratory of Helminthology, Sofia.Google Scholar
Stoitsova, S. R. (1988). Structural damage of the brush border of Hymenolepis fraterna (Cestoda) under the action of colchicine. Comptes Rendus de l'académie bulgare des Sciences 41, 127–30.Google Scholar
Stoitsova, S. R. & Dacheva, R. B. (1987). Activation of the lysosomes of the cestode, Hymenolepis fraterna, in a case of colchicine damage. Comptes Rendus de l'académie bulgare des Sciences 40, 125–7.Google Scholar
Stoitsova, S. R. & Wyroba, E. (1989). Structural variations of the surface coat along the strobila of Hymenolepis fraterna (Cestoda). Acta Parasitologica Polonica 34, 155–60.Google Scholar
Watts, S. D. M. (1986). Schistosoma mansoni: Nitrothiazolines and the male tegument. Experimental Parasitology 62, 157–68.CrossRefGoogle ScholarPubMed
Watts, S. D. M., Orpin, C. & Maccormick, C. (1979). Lysosomes and tegument pathology in the chemotherapy of schistosomiasis with 1,7bis(p–aminophenoxy)heptane (153C51). Parasitology 78, 287–94.CrossRefGoogle Scholar
Wilson, R. A. & Barnes, P. E. (1974). The tegument of Schistosoma mansoni: observations on the formation, structure and composition of cytoplasmic inclusions in relation to tegument function. Parasitology 68, 239–58.CrossRefGoogle ScholarPubMed
Wyroba, E. (1981). Alveolar system of Paramecium. I. Trapping of polycationic dye as a result of membrane impairment. Acta Histochemica 69, 132–48.CrossRefGoogle Scholar
Wyroba, E. & PrzeleÇKa, A. (1973). Studies on the surface coat of Paramecium aurelia. I. Ruthenium red staining and enzyme treatment. Zeitschrift für Zellforschung 143, 343–53.CrossRefGoogle ScholarPubMed