Hostname: page-component-586b7cd67f-t7fkt Total loading time: 0 Render date: 2024-11-26T14:41:54.884Z Has data issue: false hasContentIssue false
  • English
  • Français

Eimeria tenella: in vitro development in irradiated bovine kidney cells

Published online by Cambridge University Press:  06 April 2009

M. St J. Crane ,
D. M. Schmatz ,
S. Stevens ,
Mary C. Habbersett  and
P. K. Murray
M. St J. Crane
Affiliation:
Department of Immunoparasitology, Merck, Sharp and Dohme Research Laboratories, Rahway, New Jersey 07065
D. M. Schmatz
Affiliation:
Department of Immunoparasitology, Merck, Sharp and Dohme Research Laboratories, Rahway, New Jersey 07065
S. Stevens
Affiliation:
Department of Immunoparasitology, Merck, Sharp and Dohme Research Laboratories, Rahway, New Jersey 07065
Mary C. Habbersett
Affiliation:
Department of Immunoparasitology, Merck, Sharp and Dohme Research Laboratories, Rahway, New Jersey 07065
P. K. Murray
Affiliation:
Department of Immunoparasitology, Merck, Sharp and Dohme Research Laboratories, Rahway, New Jersey 07065
Get access Rights & Permissions [Opens in a new window]

Summary

The initial infection and first-generation development of Eimeria tenella was quantified using a cloned MDBK (Madin-Darby Bovine Kidney) cell line, irradiated with gamma radiation prior to infection, as the host cell. Irradiated cell cultures were found to be more susceptible to infection and had a greater capacity to support parasite development than non-irradiated cultures. It was suggested that the larger proportion of cells in the G2 phase of the cell cycle, the larger individual cell size and the inhibition of cell division in the irradiated cultures were all factors contributing to the increased susceptibility to infection and capacity to support parasite growth and development. The application of this technique (host cell irradiation) to the cultivation of other intracellular, protozoan parasites is discussed.

Type
Research Article
Information
Parasitology , Volume 88 , Issue 3 , June 1984 , pp. 521 - 530
Copyright
Copyright © Cambridge University Press 1984

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Beyer, T. V. & Shibalova, T. A. (1974). The increase of DNA quantity in the nuclei of chicken caecal cells harboring second generation schizonts of Eimeria tenella. Parasitologia (Leningrad) 8, 449–55. (In Russian.)Google Scholar
Crane, M. St J. & Dvorak, J. A. (1979). Trypanosoma cruzi: Interaction with vertebrate cells. DNA synthesis and growth of intracellular amastigotes and their relationship to host cell DNA synthesis and growth. Journal of Protozoology 26, 599–604.CrossRefGoogle ScholarPubMed
Darzynkiewicz, Z., Traganos, F., Sharpless, T. & Melamed, M. R. (1976). Lymphocyte stimulation: A rapid multiparameter analysis. Proceedings of the National Academy of Sciences, USA 73, 2881–5.CrossRefGoogle ScholarPubMed
Doran, D. J. (1970). Eimeria tenella: from sporozoites to oocysts in cell culture. Proceedings of the Helminthological Society of Washington 37, 84–92.Google Scholar
Doran, D. J. (1971). Increasing the yield of Eimeria tenella oocysts in cell culture. Journal of Parasitology, 57, 891–900.CrossRefGoogle ScholarPubMed
Doran, D. J. (1973). Cultivation of coccidia in avian embryos and cell cultures. In The Coccidia (ed. Hammond, D. M. and Long, P. L.), pp. 182–253. Baltimore: University Park Press.Google Scholar
Doran, D. J. (1982). Behaviour of coccidia in vitro. In The Biology of the Coccidia (ed. Long, P. L.), pp. 229–85. Baltimore: University Park Press.Google Scholar
Dvorak, J. A. & Crane, M. St J. (1981). Vertebrate cell cycle modulates infection by protozoan parasites. Science 214, 1034–6.CrossRefGoogle ScholarPubMed
Edgar, S. A. (1954). Effect of temperature on the sporulation of oocysts of the protozoan, Eimeria tenella. Transactions of the American Microbiological Society 62, 237–42.CrossRefGoogle Scholar
Fernando, M. A., Pasternak, J., Barrell, R. & Stockdale, P. H. G. (1974). Induction of host nuclear DNA synthesis in coccidia-infected chicken intestinal cells. International Journal for Parasitology 4, 267–76.CrossRefGoogle ScholarPubMed
Hyde, T. P. & Dvorak, J. A. (1973). Trypanosoma cruzi: Interaction with vertebrate cells in vitro. II. Quantitative analysis of the penetration phase. Experimental Parasitology 34, 284–94.CrossRefGoogle Scholar
Jackson, A. R. B. (1964). The isolation of viable coccidial sporozoites. Parasitology 54, 87–93.CrossRefGoogle ScholarPubMed
Latter, V. S. & Holmes, L. S. (1979). Identification of some nutrient requirements for the in vitro cultivation of Eimeria tenella. In Coccidia and further Prospects of their Control. Proceedings of the International Symposium on Coccidia, Prague, 1979, pp. 215–20.Google Scholar
Levine, S. (1960). The effect of X-irradiation on the yield of poliovirus. Virology 10, 257–67.CrossRefGoogle ScholarPubMed
Pasternak, J., Fernando, M. A., Stockdale, P. H. G. & Weber, D. (1977). Nuclear size and DNA content in host cells during first-generation schizogony of Eimeria zuernii. Parasitology 74, 199–203.CrossRefGoogle Scholar
Patton, W. H. (1965). Eimeria tenella: Cultivation of the asexual stages in cultured animal cells. Science 150, 767–9.CrossRefGoogle ScholarPubMed
Patton, W. H. & Brigman, G. P. (1979). The use of sodium taurodeoxycholate for excystation of Eimeria tenella sporozoites. Journal of Parasitology 65, 526–30.CrossRefGoogle ScholarPubMed
Puck, T. T. & Marcus, P. I. (1956). Action of X-rays on mammalian cells. Journal of Experimental Medicine 103, 653–66.CrossRefGoogle ScholarPubMed
Rosenberg, H. M. & Gregg, E. C. (1969). Kinetics of cell volume changes of murine lymphoma cells subjected to different agents in vitro. Biophysical Journal 9, 592–606.CrossRefGoogle ScholarPubMed
Schmatz, D. M., Crane, M. St J. & Murray, P. K. (1984). Purification of Eimeria sporozoites by DE-52 anion exchange chromatography. Journal of Protozoology (in the Press).CrossRefGoogle ScholarPubMed
Schmatz, D. M. & Murray, P. K. (1982). Cultivation of Trypanosoma cruzi in irradiated muscle cells: improved synchronization and enhanced trypomastigote production. Parasitology 85, 115–25.CrossRefGoogle ScholarPubMed
Tannenbaum, E., Cassidy, M., Alabaster, O. & Herman, C. (1978). Measurement of cellular DNA mass by flow microfluorometry with use of a biological internal standard. Journal of Histochemistry and Cytochemistry 26, 145–8.CrossRefGoogle ScholarPubMed
Whitmore, G. F., Till, J. E., Gwatkin, R. B. L., Siminovich, L. & Graham, A. F. (1958). Increase of cellular constituents in X-irradiated mammalian cells. Biochimica et biophysica acta 30, 583–90.CrossRefGoogle ScholarPubMed