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Endotube–brush border complexes dissected from the intestines of Haemonchus contortus and Ancylostoma caninum

Published online by Cambridge University Press:  06 April 2009

E. A. Munn  and
C. A. Greenwood
E. A. Munn
Affiliation:
Agricultural Research Council, Institute of Animal Physiology, Babraham, Cambridge CB2 A AT
C. A. Greenwood
Affiliation:
Agricultural Research Council, Institute of Animal Physiology, Babraham, Cambridge CB2 A AT
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Summary

A procedure for the blunt dissection of the syncytial intestine of Haemonchus contortus to separate the endotube–brush border complex is described. A similar complex can be obtained from the partially cellular intestine of Ancylostoma caninum. The structural features of the complexes from the two sources are compared. The main differences are in the retention of terminal bars by the endotube from A. caninum and in the extent and organization of the microfilaments associated with the basal side of the endotubes.

Type
Research Article
Information
Parasitology , Volume 87 , Issue 1 , August 1983 , pp. 129 - 137
Copyright
Copyright © Cambridge University Press 1983

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References

REFERENCES

Andreassen, J. (1968). Fine structure of the intestine of the nematodes, Ancylostoma caninum and Phocanema decipiens. Zeitschrift für Parasitenkunde 30, 318–36.CrossRefGoogle ScholarPubMed
Borgers, M., De Nollin, S., De Brabander, M. & Thienpont, D. (1975). Influence of the anthelmintic Mebendazole on microtubules and intracellular organelle movement in nematode intestinal cells. American Journal of Veterinary Research 36, 1153–66.Google Scholar
Browne, H. G. & Chowdhury, A. B. (1959). The ultrastructure of the intestinal wall of Ancylostoma caninum. Journal of Parasitology 45, 241–7.CrossRefGoogle Scholar
Colam, J. B. (1971). Studies on gut ultrastructure and digestive physiology in Cyathostoma lari (Nematoda: Strongylida). Parasitology 62, 273–83.CrossRefGoogle ScholarPubMed
Lee, C. C. (1969). Ancylostoma caninum: fine structure of intestinal epithelium. Experimental Parasitology 24, 336–47.CrossRefGoogle ScholarPubMed
Miller, J. H. (1967). Fine structure of the striated border of the intestinal cells of Ancylostoma caninum. Journal of Parasitology 53, 94–9.CrossRefGoogle Scholar
Munn, E. A. (1977). A helical, polymeric extracellular protein associated with the luminal surface of Haemonchus contortus intestinal cells. Tissue and Cell 9, 23–34.CrossRefGoogle ScholarPubMed
Munn, E. A. (1981). The endotube, a macroscopic intracellular structure from the syncytial intestine of the parasitic nematode Haemonchus contortus. Journal of Physiology 319, 7–8P.Google Scholar
Munn, E. A. & Greenwood, C. A. (1982). The occurrence of submicrovillar endotube (modified terminal web) and associated cytoskeletal structures in the intestinal epithelia of nematodes. Proceedings of the Royal Society, B(in the Press).Google Scholar
Smith, K. & Harness, E. (1972). The ultrastructure of the adult stage of Trichostrongylus colubriformis and Haemonchus placei. Parasitology 64, 173–9.CrossRefGoogle ScholarPubMed