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Functional antigens of Trichuris muris released during in vitro maintenance: their immunogenicity and partial purification

Published online by Cambridge University Press:  06 April 2009

S. N. Jenkins  and
D. Wakelin
S. N. Jenkins
Affiliation:
Wellcome Laboratories for Experimental Parasitology, University of Glasgow, Bearsden Road, Bearsden, Glasgow G61 1QH
D. Wakelin
Affiliation:
Wellcome Laboratories for Experimental Parasitology, University of Glasgow, Bearsden Road, Bearsden, Glasgow G61 1QH
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Summary

The immunogenicity of the secretory exo-antigen (EXA) originating in the stichosome of Trichuris muris has been investigated. High levels of immunity to challenge were produced using EXA (100 μg) by subcutaneous administration. Vaccination with a range of doses of EXA in Freunds' Incomplete Adjuvant (FIA) showed a dose response; 1 μg of EXA reduced the worm burden by 50% and doses of 10 μg or more reduced the worm burden by 80–90% at Day 9 post-infection. Worms recovered from vaccinated mice were significantly smaller than those from controls. A precipitin line identifiable with EXA was shown to immunize mice against T. muris challenge by vaccination. The protective antigen contained in EXA has been partially isolated using gel filtration, ion exchange filtration and isoelectric focusing and has been shown to be a protein of 30000 molecular weight having a pi of 6·8–7·3.

Type
Research Article
Information
Parasitology , Volume 86 , Issue 1 , February 1983 , pp. 73 - 82
Copyright
Copyright © Cambridge University Press 1983

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