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Genetic studies on Plasmodium chabaudi: recombination between enzyme markers

Published online by Cambridge University Press:  06 April 2009

D. Walliker ,
R. Carter  and
A. Sanderson
D. Walliker
Affiliation:
Protozoan Genetics Unit, Institute of Animal Genetics, West Mains Road, Edinburgh, EH9 3JN
R. Carter
Affiliation:
Protozoan Genetics Unit, Institute of Animal Genetics, West Mains Road, Edinburgh, EH9 3JN
A. Sanderson
Affiliation:
Protozoan Genetics Unit, Institute of Animal Genetics, West Mains Road, Edinburgh, EH9 3JN
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Extract

Two lines of Plasmodium chabaudi differing in three characters have been crossed, using a technique previously described for P. yoelii. One line, termed 47AS, was characterized by an electrophoretic form of 6-phosphogluconate dehydrogenase, denoted 6PGD-2, a form of lactate dehydrogenase, denoted LDH-3, and was pyrimethamine-resistant. The second line, termed 10AJ, possessed enzyme forms 6PGD-3 and LDH-2 and was pyrimethamine-sensitive. The cross was made by permitting mosquitoes to feed on a mixture of the two lines and infecting rodents with the resulting sporozoites. The products of the cross were cloned by dilution and examined for enzyme-type and drug-response. Results showed that recombination had occurred between each of the three characters. Clones characterized by 6PGD-2/LDH-2 and 6PGD-3/LDH-3 demonstrated recombination between the enzyme markers. The drug-resistance character segregated independently of either enzyme marker.

Type
Research Article
Information
Parasitology , Volume 70 , Issue 1 , February 1975 , pp. 19 - 24
Copyright
Copyright © Cambridge University Press 1975

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