Hostname: page-component-586b7cd67f-2brh9 Total loading time: 0 Render date: 2024-11-22T21:43:16.123Z Has data issue: false hasContentIssue false
  • English
  • Français

Genetic studies on Plasmodium chabaudi: recombination between enzyme markers

Published online by Cambridge University Press:  06 April 2009

D. Walliker ,
R. Carter  and
A. Sanderson
D. Walliker
Affiliation:
Protozoan Genetics Unit, Institute of Animal Genetics, West Mains Road, Edinburgh, EH9 3JN
R. Carter
Affiliation:
Protozoan Genetics Unit, Institute of Animal Genetics, West Mains Road, Edinburgh, EH9 3JN
A. Sanderson
Affiliation:
Protozoan Genetics Unit, Institute of Animal Genetics, West Mains Road, Edinburgh, EH9 3JN
Get access Rights & Permissions [Opens in a new window]

Extract

Two lines of Plasmodium chabaudi differing in three characters have been crossed, using a technique previously described for P. yoelii. One line, termed 47AS, was characterized by an electrophoretic form of 6-phosphogluconate dehydrogenase, denoted 6PGD-2, a form of lactate dehydrogenase, denoted LDH-3, and was pyrimethamine-resistant. The second line, termed 10AJ, possessed enzyme forms 6PGD-3 and LDH-2 and was pyrimethamine-sensitive. The cross was made by permitting mosquitoes to feed on a mixture of the two lines and infecting rodents with the resulting sporozoites. The products of the cross were cloned by dilution and examined for enzyme-type and drug-response. Results showed that recombination had occurred between each of the three characters. Clones characterized by 6PGD-2/LDH-2 and 6PGD-3/LDH-3 demonstrated recombination between the enzyme markers. The drug-resistance character segregated independently of either enzyme marker.

Type
Research Article
Information
Parasitology , Volume 70 , Issue 1 , February 1975 , pp. 19 - 24
Copyright
Copyright © Cambridge University Press 1975

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Carter, R., (1973). Enzyme variation in Plasmodium berghei and Plasmodium vinckei. Parasitology 66, 297–307.Google Scholar
Carter, R., & Walliker, D., (1975). New observations on the malaria parasites of rodents of the Central African Republic; Plasmodium vinckei petteri subsp.nov. and Plasmodium chabaudi Landau, 1965. Annals of Tropical Medicine and Parasitology (in the Press).CrossRefGoogle ScholarPubMed
Killick-Kendrick, R., (1974). Parasitic protozoa of the blood of rodents. II. Haemogregarines, malaria parasites and piroplasms of rodents: an annotated checklist and host index. Acta tropica, Separatum vol. 31 (1), 28.Google Scholar
Lumsden, W. H. R., Cunningham, M. P., Webber, W. A. F., van Hoeve, K., Knight, R. H., & Simmons, V., (1965). Some effects of hydrogen ion concentration on trypanosome numbers and infectivity. Experimental Parasitology 16, 8–17.CrossRefGoogle ScholarPubMed
Oxbrow, A. I., (1973). Strain specific immunity to Plasmodium berghei: a new genetic marker. Parasitology 67, 17–27.CrossRefGoogle ScholarPubMed
Sinden, R. E., & Canning, E. U., (1973). Ultrastructure and cytochemistry of nuclear division in Plasmodium. Progress in Protozoology, p. 385. (Abstract of papers read at the Fourth International Congress of Protozoology, Clermont-Ferrand, 1973.)Google Scholar
Walliker, D., (1972). An infection of Plasmodium berghei derived from sporozoites of a single oocyst. Transactions of the Royal Society of Tropical Medicine and Hygiene 4, 543.Google Scholar
Walliker, D., Carter, R., & Morgan, S., (1971). Genetic recombination in malaria parasites. Nature, London 232, 561–2.Google Scholar
Walliker, D., Carter, R., & Morgan, S., (1973). Genetic recombination in Plasmodium berghei. Parasitology 66, 309–320.Google Scholar
Yoeli, M., Upmanis, R. S., & Most, H., (1969). Drug-resistance transfer among rodent plasmodia. 1. Acquisition of resistance to pyrimethamine by a drug-sensitive strain of Plasmodium berghei in the course of its concomitant development with a pyrimethamine-resistant P. vinckei strain. Parasitology 59, 429–47.CrossRefGoogle ScholarPubMed