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Identification and quantification of rodent malaria strains and species using gene probes

Published online by Cambridge University Press:  06 April 2009

G. Snounou ,
T. Bourne ,
W. Jarra ,
S. Viriyakosol  and
K. N. Brown
G. Snounou
Affiliation:
Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
T. Bourne
Affiliation:
Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
W. Jarra
Affiliation:
Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
S. Viriyakosol
Affiliation:
Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
K. N. Brown
Affiliation:
Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
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Extract

A DNA probe PCsv4 and a subclone thereof PCsv4.1, hybridize specifically to rodent malaria DNA. DNA purified from a small volume (10 μ1) of infected mouse blood was used to determine the composition of the parasite population present. The hybridization signal following PCsv4 probing of slot-blotted DNA correlated directly with parasitaemia. The hybridization pattern and Intensity, resulting from probing restriction enzyme digested and Southern-blotted genomic DNA, determined the identity of the infecting parasite line(s), and provided a semi-quantitative measure of parasite burden. Fifteen parasite lines representative of all four Plasmodium species infecting rodents can be differentiated in this way.

Keywords

Plasmodiumrodent malariaDNA probeRFLPdiagnosis.
Type
Research Article
Information
Parasitology , Volume 105 , Issue 1 , August 1992 , pp. 21 - 27
Copyright
Copyright © Cambridge University Press 1992

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