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Immune modulation by a high molecular weight fraction from the rat tapeworm Hymenolepis diminuta

Published online by Cambridge University Press:  22 December 2004

A. WANG  and
D. M. McKAY
A. WANG
Affiliation:
Intestinal Disease Research Programme, McMaster University, 1200 Main Street West, Hamilton, Ontario, Canada L8N 3Z5
D. M. McKAY
Affiliation:
Intestinal Disease Research Programme, McMaster University, 1200 Main Street West, Hamilton, Ontario, Canada L8N 3Z5
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Abstract

The host-parasite relationship is exquisitely specific. In exploiting the host niche, a variety of helminth parasites have been shown to directly manipulate their hosts' immune responses. We assessed the ability of a whole-worm extract of Hymenolepis diminuta to modulate immune cell activation. Immune cells isolated from human blood or rodent spleens were activated with the T cell mitogen, concanavalin A (Con A)±H. diminuta extract and cytokine production (i.e. IL-2, -4, -10, -12) and proliferation assessed by ELISA and [3H]thymidine incorporation 24 and 72 h post-treatment, respectively. Co-treatment with the H. diminuta extract (100 μg protein/ml) virtually abolished Con A-induced immune cell proliferation, which was not due to increased apoptosis. Boiling of the worm extract reduced its anti-proliferative effect and fractionation indicated that a >50 kDa component was predominantly responsible for the inhibition of Con A-induced immune cell proliferation. Cytokine determinations revealed that the H. diminuta extract significantly reduced Con A-stimulated IL-2 and IL-4, but enhanced the production of IFNγ, IL-12 and IL-10. The increased IL-12 was due to an LPS contaminant in the extract and a helminth-derived ‘IL-12’-like peptide that bound in the ELISA and Western blots. In contrast, a H. diminuta–derived factor directly stimulated IL-10 production by murine splenocytes, and contaminating LPS synergistically enhanced the production of IL-10. Thus, H. diminuta has the potential to block stimulated T cell proliferation and, by inhibiting IL-4 and promoting IL-10 production, may bias the immune environment towards one of immunoregulation and away from IL-4 dominated T helper 2 type events.

Keywords

cestodecytokineshost-parasite interaction
Type
Research Article
Information
Parasitology , Volume 130 , Issue 5 , May 2005 , pp. 575 - 585
Copyright
© 2005 Cambridge University Press

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