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Immunocytochemical localization of serotonin (5-HT) in the nervous system of the hydatid organism, Echinococcus granulosus (Cestoda, Cyclophyllidea)

Published online by Cambridge University Press:  06 April 2009

D. J. A. Brownlee ,
I. Fairweather ,
C. F. Johnston  and
M. T. Rogan
D. J. A. Brownlee
Affiliation:
School of Biology and Biochemistry, The Queen's University of Belfast, Belfast BT7 INN, Northern Ireland
I. Fairweather
Affiliation:
School of Biology and Biochemistry, The Queen's University of Belfast, Belfast BT7 INN, Northern Ireland
C. F. Johnston
Affiliation:
School of Clinical Medicine, The Queen's University of Belfast, Belfast BT7 INN, Northern Ireland
M. T. Rogan
Affiliation:
Department of Biological Sciences, University of Salford, Salford M5 4WT, England
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Summary

The localization and distribution of the serotoninergic components of the nervous system in the hydatid organism, Echinococcus granulosus, were determined by immunocytochemical techniques in conjunction with confocal scanning laser microscopy (CSLM). The distribution of serotonin immunoreactivity (IR) paralleled that previously described for cholinesterase activity, although it was more widespread. Nerve cell bodies and nerve fibres immunoreactive for 5-HT were present throughout the central nervous system (CNS), occurring in the paired lateral, posterior lateral and rostellar ganglia, their connecting commissures and nerve rings in the scolex and in the ten longitudinal nerve cords that run posteriorly throughout the body of the worm. A large population of nerve cell bodies was associated with the lateral nerve cords. In the peripheral nervous system (PNS), immunoreactive nerve fibres occurred in well-developed nerve plexuses innervating the somatic musculature and the musculature of the rostellum and suckers. The genital atrium and associated reproductive ducts were richly innervated with serotoninergic nerve cell bodies and nerve fibres.

Keywords

Echinococcus granulosusCestodaCyclophyllideahydatid organismnervous systemserotoninimmunocytochemistryconfocal scanning laser microscopy
Type
Research Article
Information
Parasitology , Volume 109 , Issue 2 , August 1994 , pp. 233 - 241
Copyright
Copyright © Cambridge University Press 1994

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