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The in vitro anthelmintic activity of the ethanol leaf extracts of Terminalia catappa L. on Fasciola gigantica

Published online by Cambridge University Press:  14 August 2017

PANAT ANURACPREEDA ,
RUNGLAWAN CHAWENGKIRTTIKUL ,
ARIN NGAMNIYOM ,
BUSABA PANYARACHUN ,
PANUPONG PUTTARAK ,
PREEYAPORN KOEDRITH  and
NOPPADOL INTARATAT
PANAT ANURACPREEDA*
Affiliation:
Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhon Pathom 73170, Thailand
RUNGLAWAN CHAWENGKIRTTIKUL
Affiliation:
Department of Microbiology, Faculty of Science, Mahidol University, Rama VI Rd., Bangkok 10400, Thailand
ARIN NGAMNIYOM
Affiliation:
Faculty of Environmental Culture and Ecotourism, Srinakharinwirot University, Sukhumvit 23, Klongtoey Wattana, Bangkok 10110, Thailand
BUSABA PANYARACHUN
Affiliation:
Department of Anatomy, Faculty of Medicine, Srinakharinwirot University, Sukhumvit 23, Klongtoey Wattana, Bangkok 10110, Thailand
PANUPONG PUTTARAK
Affiliation:
Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand
PREEYAPORN KOEDRITH
Affiliation:
Faculty of Environment and Resource Studies, Mahidol University, Phuttamonthon District, Nakhon Pathom 73170, Thailand
NOPPADOL INTARATAT
Affiliation:
Department of Anatomy, Faculty of Medicine, Srinakharinwirot University, Sukhumvit 23, Klongtoey Wattana, Bangkok 10110, Thailand
*
*Corresponding author: Institute of Molecular Biosciences, Mahidol University, 25/25 Phuttamonthon 4 Road, Salaya, Nakhon Pathom 73170, Thailand. E-mail: Panat1@yahoo.com, panat.anu@mahidol.ac.th
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Summary

At present, there are no medicinal plant extracts currently available for treatment and control of fasciolosis. The present work could provide, for the first study, conclusions on the in vitro fasciolicidal properties of the ethanol extract of Terminalia catappa L. (TcCE) leaves against adult Fasciola gigantica after incubation with RPMI-1640 medium containing the TcCE at various concentrations and times when compared with triclabendazole (TCZ). The relative motility and survival index values of the TcCE-treated flukes decreased at a more rapid rate than the TCZ-treated flukes. The death of the parasites was observed after exposed to TcCE at 3 h incubation with 400, 800 and 1000 µg mL−1, and at 6 h incubation in 100 and 200 µg mL−1. Vacuolization, blebbings and partial disruption on the parasites’ tegument were observed by light microscopy. When examined by scanning electron microscopy, TcCE caused similar tegumental alterations in the parasites as those observed in TCZ treatment but with larger damage at comparative incubation periods, consisting of swelling, blebbing, disrupted blebs, loss of spines, leading to the erosion, lesion and eventual disruption of the total tegument. Therefore, the TcCE may exert its fasciolicidal effect against F. gigantica by initially causing the tegumental alteration.

Keywords

FasciolosisFasciola giganticaTerminalia catappa L.anthelmintic activitymotilitysurvivaltegumentlight microscopyscanning electron microscopy
Type
Research Article
Information
Parasitology , Volume 144 , Issue 14 , December 2017 , pp. 1931 - 1942
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Copyright
Copyright © Cambridge University Press 2017 

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