Hostname: page-component-586b7cd67f-t7fkt Total loading time: 0 Render date: 2024-11-20T13:33:35.583Z Has data issue: false hasContentIssue false
  • English
  • Français

The in vitro cultivation of P. falciparum ookinetes, and their enrichment on Nycodenz® density gradients

Published online by Cambridge University Press:  06 April 2009

E. H. Carter ,
A. Suhrbier ,
P. J. A. Beckers  and
R. E. Sinden
E. H. Carter
Affiliation:
Molecular and Cellular Parasitology Research Group, Department of Pure and Applied Biology, Imperial College, London SW7 2AZ
A. Suhrbier
Affiliation:
Molecular and Cellular Parasitology Research Group, Department of Pure and Applied Biology, Imperial College, London SW7 2AZ
P. J. A. Beckers
Affiliation:
Department of Medical Parasitology, University of Nijmegen, Netherlands
R. E. Sinden*
Affiliation:
Molecular and Cellular Parasitology Research Group, Department of Pure and Applied Biology, Imperial College, London SW7 2AZ
*
*Reprint requests to Dr R. E. Sinden.
Get access Rights & Permissions [Opens in a new window]

Summary

Employing a simple method of growing ookinetes of Plasmodium falciparum in culture, 40% of mature gametocytes convert to macrogametes, 4% reach the retort-form ookinete stage and 0·45% become mature ookinetes. A single-step gradient centrifugation method on 12·5% Nycodenz® enriches both gametocytes and ookinetes.

Type
Research Article
Information
Parasitology , Volume 95 , Issue 1 , August 1987 , pp. 25 - 30
Copyright
Copyright © Cambridge University Press 1987

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Aikawa, M., Carter, R., Ito, Y. & Nijhout, M. (1984). New observations on gametogenesis, fertilization and zygote transformation in Plasmodium gallinaceum. Journal of Protozoology 31, 403–13.Google Scholar
Carter, R., Gwadz, R. W. & McAuliffe, M. (1979). Plasmodium gallinaceum transmission blocking immunity in chickens. 1. Comparative immunogenicity of gametocyte and gamete containing preparations. Experimental Parasitology 47, 185–93.Google Scholar
Hawking, F., Wilson, M. E. & Gammage, K. (1971). Evidence for the cyclic development of short-lived maturity in the gametocytes of Plasmodium falciparum. Transactions of the Royal Society of Tropical Hygiene and Medicine 65, 549–59.Google Scholar
Janse, C. J., Mons, B., Rouwenhorst, R. J., Van der Klooster, P. F. J., Overdulve, J. P. & Van der Kaay, H. J. (1985). In vitro formation of ookinetes and functional maturity of Plasmodium berghei gametocytes. Parasitology 91, 14–29.Google Scholar
Knight, A. & Sinden, R. E. (1983). The purification of gametocytes of Plasmodium falciparum and P. yoelii nigeriensis by colloidal silica gel gradient centrifugation. Transactions of the Royal Society of Tropical Hygiene and Medicine 76, 503–9.CrossRefGoogle Scholar
Kumar, N. & Carter, R. (1984). Biosynthesis of target antigens of antibodies blocking transmission of Plasmodium falciparum. Molecular and Biochemical Parasitology 13, 333–42.CrossRefGoogle ScholarPubMed
Ponnudurai, T., Lensen, A. H. W., Leeuwbnberg, A. D. E. M. & Meuwissen, J. H. E. Th. (1982). Cultivation of fertile Plasmodium falciparum gametocytes in semi-automated systems. 1. Static cultures. Transactions of the Royal Society of Tropical Hygiene and Medicine 76, 812–18.CrossRefGoogle ScholarPubMed
Sinden, R. E., Hartley, R. H. & Winger, L. (1985). The development of Plasmodium ookinetes in vitro: an ultrastructual study including a description of the meiotic division. Parasitology 91, 227–44.CrossRefGoogle Scholar
Sinden, R. E., Winger, L., Carter, E. H. C, Hartley, R. H., Tirawanchai, N., Da vies, C. S. & Sluiters, J. F. (1987). Ookinete antigens of Plasmodium berghei: a light and electron-microscope immunogold study of the expression of the 21 KDa determinant recognised by a transmission blocking antibody. Proceedings of the Royal Society of London, (in the Press).Google Scholar
Vermeulen, A. N., Ponnudurai, T., Beckers, P. J. A., Verhave, J. P., Smits, M. A. & Meuwissen, J. H. E. Th. (1985). Sequential expression of antigens on sexual stages of Plasmodium falciparum accessible to transmission blocking antibodies in the mosquito. Journal of Experimental Medicine 162, 1460–76.Google Scholar
Vermeulen, A. N., van Deursen, J. M. A., Brakenhoff, R. H., Lensen, T. H. W., Ponnudurai, T. & Meuwissen, J. E. H. Th. (1986). Characterization of Plasmodium falciparum sexual stage antigens and their biosynthesis in synchronised gametocyte cultures. Molecular and Biochemical Parasitology (in the Press).CrossRefGoogle ScholarPubMed
Weiss, M. M. & Vanderberg, J. P. (1977). Studies on Plasmodium ookinetes. II. In vitro formation of Plasmodium berghei ookinetes. Journal of Parasitology 63, 932–4.Google Scholar
Winger, L., Smith, J. E., Nicholas, J., Carter, E. H., Tirawanchai, N. & Sinden, R. E. (1987). Ookinete antigens of Plasmodium berghei; Appearance on the zygote of a Mr21 K surface determinant identified by transmission blocking antibodies. Parasite Immunology (in the Press).Google Scholar