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Isolation and biological and molecular characterization of Neospora caninum (NC-SP1) from a naturally infected adult asymptomatic cattle (Bos taurus) in the state of São Paulo, Brazil

Published online by Cambridge University Press:  11 January 2017

SOLANGE OLIVEIRA ,
RODRIGO MARTINS SOARES ,
JULIANA AIZAWA ,
HERBERT SOUSA SOARES ,
DANIELA PONTES CHIEBAO ,
LUIS MIGUEL ORTEGA-MORA ,
JAVIER REGIDOR-CERRILLO ,
NATÁLIA QUADROS BRESSA SILVA ,
SOLANGE MARIA GENNARI  and
HILDA FÁTIMA JESUS PENA
SOLANGE OLIVEIRA
Affiliation:
Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine, University of São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, CEP 05508-270, São Paulo, Brazil
RODRIGO MARTINS SOARES
Affiliation:
Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine, University of São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, CEP 05508-270, São Paulo, Brazil
JULIANA AIZAWA
Affiliation:
Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine, University of São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, CEP 05508-270, São Paulo, Brazil
HERBERT SOUSA SOARES
Affiliation:
Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine, University of São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, CEP 05508-270, São Paulo, Brazil
DANIELA PONTES CHIEBAO
Affiliation:
Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine, University of São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, CEP 05508-270, São Paulo, Brazil
LUIS MIGUEL ORTEGA-MORA
Affiliation:
SALUVET, AnimalHealth Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain
JAVIER REGIDOR-CERRILLO
Affiliation:
SALUVET, AnimalHealth Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain
NATÁLIA QUADROS BRESSA SILVA
Affiliation:
Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine, University of São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, CEP 05508-270, São Paulo, Brazil
SOLANGE MARIA GENNARI
Affiliation:
Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine, University of São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, CEP 05508-270, São Paulo, Brazil
HILDA FÁTIMA JESUS PENA*
Affiliation:
Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine, University of São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, CEP 05508-270, São Paulo, Brazil
*
*Corresponding author: Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine, University of São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, CEP 05508-270, São Paulo, Brazil. E-mail: hfpena@usp.br
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Summary

The biological and genetic diversity of Neospora caninum is very limited because of availability of only a few viable isolates worldwide. This study describes the isolation and biological and molecular characterization of a new viable isolate of N. caninum (NC-SP1), from a cattle in Brazil. Approximately 400 g of brain from a naturally infected adult male cattle from an abattoir was fed to a 2-month-old dog. Neospora-like oocysts were observed on day 7 post-inoculation (PI) and the duration of oocyst shedding was 14 days. The DNA obtained from oocysts was characterized molecularly and the final sequence was 99% identical to homologous sequences of N. caninum available in GenBank®. For bioassay, gerbils (Meriones unguiculatus) were orally inoculated with 10 100 and 1000 oocysts; all gerbils remained clinically normal but developed N. caninum antibodies 14 days PI. Cell culture isolation was successful using the brain homogenate from one of the gerbils and tachyzoites were observed 24 days PI. Microsatellite genotyping revealed a unique genetic profile for this new reference isolate.

Keywords

Neosporosiscattlebioassayoocyst sheddinggerbilsmicrosatellite analysis
Type
Research Article
Information
Parasitology , Volume 144 , Issue 6 , May 2017 , pp. 707 - 711
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Copyright
Copyright © Cambridge University Press 2017 

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