Hostname: page-component-77c89778f8-vsgnj Total loading time: 0 Render date: 2024-07-18T06:35:32.889Z Has data issue: false hasContentIssue false
  • English
  • Français

Isolation and genetic characterization of Neospora caninum from asymptomatic calves in Spain

Published online by Cambridge University Press:  04 November 2008

J. REGIDOR-CERRILLO ,
M. GÓMEZ-BAUTISTA ,
J. PEREIRA-BUENO ,
G. ADURIZ ,
V. NAVARRO-LOZANO ,
V. RISCO-CASTILLO ,
A. FÉRNANDEZ-GARCÍA ,
S. PEDRAZA-DÍAZ  and
L. M. ORTEGA-MORA
J. REGIDOR-CERRILLO
Affiliation:
SALUVET, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain
M. GÓMEZ-BAUTISTA
Affiliation:
SALUVET, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain
J. PEREIRA-BUENO
Affiliation:
SALUVET, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain
G. ADURIZ
Affiliation:
Departamento de Producción y Sanidad Animal, NEIKER-Tecnalia, Instituto Vasco de Investigación y Desarrollo Agrario, Derio, 48160, Bizkaia, Spain
V. NAVARRO-LOZANO
Affiliation:
SALUVET, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain
V. RISCO-CASTILLO
Affiliation:
SALUVET, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain
A. FÉRNANDEZ-GARCÍA
Affiliation:
SALUVET, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain
S. PEDRAZA-DÍAZ
Affiliation:
SALUVET, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain
L. M. ORTEGA-MORA*
Affiliation:
SALUVET, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain
*
*Corresponding author: SALUVET, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040-Madrid, Spain. Tel: +34 91 3944069. Fax: +34 91- 944098. E-mail: luis.ortega@vet.ucm.es
Get access Rights & Permissions [Opens in a new window]

Summary

Neospora caninum is a cyst-forming parasite that causes abortion in cattle. Despite this parasite's ubiquitous distribution and wide host range, the number of N. caninum isolates obtained to date is limited. In vitro isolation of the parasite is arduous and often unsuccessful. In addition, most isolates have been obtained from clinically affected hosts and therefore could be biased towards more virulent isolates. In this report, an improved isolation approach from transplacentally infected newborn calves was undertaken and 9 new isolates were obtained. Moreover, a microsatellite technique was applied to investigate the genetic diversity of these isolates. Most isolates showed specific genetic profiles. However, the Nc-Spain10 isolate was identical to the previously described Nc-Spain1H isolate and Nc-Spain3H was identical to Nc-Spain4H. These isolates were likely to have identical genotypes because they were isolated from distinct calves of the same herd. Future pathogenic characterization of these isolates will contribute to the investigation of the relationship between isolate virulence and the outcome of infection, as well as other epidemiological features, such as transmission.

Keywords

Neospora caninumisolationasymptomatic calvesSpaingenetic characterization
Type
Research Article
Information
Parasitology , Volume 135 , Issue 14 , December 2008 , pp. 1651 - 1659
Copyright
Copyright © 2008 Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Álvarez-García, G., Pereira-Bueno, J., Gómez-Bautista, M. and Ortega-Mora, L. M. (2002). Pattern of recognition of Neospora caninum tachyzoite antigens by naturally infected pregnant cattle and aborted foetuses. Veterinary Parasitology 107, 15–27. doi:10.1016/S0304-4017(02)00091-2.CrossRefGoogle ScholarPubMed
Atkinson, R., Harper, P. A., Ryce, C., Morrison, D. A. and Ellis, J. T. (1999). Comparison of the biological characteristics of two isolates of Neospora caninum. Parasitology 118, 363–370.CrossRefGoogle ScholarPubMed
Buxton, D., Maley, S. W., Wright, S., Thomson, K. M., Rae, A. G. and Innes, E. A. (1998). The pathogenesis of experimental neosporosis in pregnant sheep. Journal of Comparative Pathology 118, 267–279.CrossRefGoogle ScholarPubMed
Canada, N., Meireles, C. S., Mezo, M., Gónzalez-Warleta, M., Correia da Costa, J. M., Sreekumar, C., Hill, D. E., Miska, K. B. and Dubey, J. P. (2004). First isolation of Neospora caninum from an aborted bovine fetus in Spain. Journal of Parasitology 90, 863–864.CrossRefGoogle ScholarPubMed
Collantes-Fernández, E., López-Pérez, I., Álvarez-García, G. and Ortega-Mora, L. M. (2006). Temporal distribution and parasite load kinetics in blood and tissues during Neospora caninum infection in mice. Infection and Immunity 74, 2491–2494. doi: 10.1128/IAI.74.4.2491-2494.2006.CrossRefGoogle ScholarPubMed
Conrad, P. A., Barr, B. C., Sverlow, K. W., Anderson, M., Daft, B., Kinde, H., Dubey, J. P., Munson, L. and Ardans, A. (1993). In vitro isolation and characterization of a Neospora sp. from aborted bovine foetuses. Parasitology 106, 239–249.CrossRefGoogle ScholarPubMed
Dubey, J. P., Hattel, A. L., Lindsay, D. S. and Topper, M. J. (1988). Neonatal Neospora caninum infection in dogs: isolation of the causative agent and experimental transmission. Journal of the American Veterinary Medical Association 193, 1259–1263.Google ScholarPubMed
Dubey, J. P. and Schares, G. (2006). Diagnosis of bovine neosporosis. Veterinary Parasitology 140, 1–34. doi:10.1016/j.vetpar.2006.03.035.CrossRefGoogle ScholarPubMed
Dubey, J. P., Schares, G. and Ortega-Mora, L. M. (2007). Epidemiology and control of neosporosis and Neospora caninum. Clinical Microbiology Reviews 20, 323–367. doi: 10.1128/CMR.00031-06.CrossRefGoogle ScholarPubMed
Koyama, T., Kobayashi, Y., Omata, Y., Yamada, M., Furuoka, H., Maeda, R., Matsui, T., Saito, A. and Mikami, T. (2001). Isolation of Neospora caninum from the brain of a pregnant sheep. Journal of Parasitology 87, 1486–1488.CrossRefGoogle ScholarPubMed
Miller, C. M., Quinn, H. E., Windsor, P. A. and Ellis, J. T. (2002). Characterisation of the first Australian isolate of Neospora caninum from cattle. Australian Veterinary Journal 80, 620–625.CrossRefGoogle ScholarPubMed
Okeoma, C. M., Williamson, N. B., Pomroy, W. E., Stowell, K. M. and Gillespie, L. M. (2004). Isolation and molecular characterisation of Neospora caninum in cattle in New Zealand. New Zealand Veterinary Journal 52, 364–370.CrossRefGoogle ScholarPubMed
Pereira-Bueno, J., Quintanilla-Gozalo, A., Pérez-Pérez, V., Espi-Felgueroso, A., Álvarez-García, G., Collantes-Fernández, E. and Ortega-Mora, L. M. (2003). Evaluation by different diagnostic techniques of bovine abortion associated with Neospora caninum in Spain. Veterinary Parasitology 111, 143–152. doi:10.1016/S0304-4017(02)00361-8.CrossRefGoogle ScholarPubMed
Pérez-Zaballos, F. J., Ortega-Mora, L. M., Álvarez-Garcia, G., Collantes-Fernández, E., Navarro-Lozano, V., García-Villada, L. and Costas, E. (2005). Adaptation of Neospora caninum isolates to cell-culture changes: an argument in favor of its clonal population structure. Journal of Parasitology 91, 507–510.CrossRefGoogle ScholarPubMed
Piergili Fioretti, D., Rosignoli, L., Ricci, G., Moretti, A., Pasquali, P. and Polidori, G. A. (2000). Neospora caninum infection in a clinically healthy calf: parasitological study and serological follow-up. Journal of Veterinary Medicine Series B 47, 47–53. doi:10.1046/j.1439-0450.2000.00304.xCrossRefGoogle Scholar
Regidor-Cerrillo, J., Pedraza-Díaz, S., Gómez-Bautista, M. and Ortega-Mora, L. M. (2006). Multilocus microsatellite analysis reveals extensive genetic diversity in Neospora caninum. Journal of Parasitology 92, 517–524.CrossRefGoogle ScholarPubMed
Saeij, J. P., Boyle, J. P. and Boothroyd, J. C. (2005). Differences among the three major strains of Toxoplasma gondii and their specific interactions with the infected host. Trends in Parasitology 21, 476–481. doi:10.1016/j.pt.2005.08.001.CrossRefGoogle ScholarPubMed
Sawada, M., Kondo, H., Tomioka, Y., Park, C. H., Morita, T., Shimada, A. and Umemura, T. (2000). Isolation of Neospora caninum from the brain of a naturally infected adult dairy cow. Veterinary Parasitology 90, 247–252. doi:10.1016/S0304-4017(00)00248-X.CrossRefGoogle ScholarPubMed
Schock, A., Innes, E. A., Yamane, I., Latham, S. M. and Wastling, J. M. (2001). Genetic and biological diversity among isolates of Neospora caninum. Parasitology 123, 13–23.CrossRefGoogle ScholarPubMed
Vianna, M. C., Sreekumar, C., Miska, K. B., Hill, D. E. and Dubey, J. P. (2005). Isolation of Neospora caninum from naturally infected white-tailed deer (Odocoileus virginianus). Veterinary Parasitology 129, 253–257. doi:10.1016/j.vetpar.2004.02.031.CrossRefGoogle ScholarPubMed
Yamane, I., Kokuho, T., Shimura, K., Eto, M., Shibahara, T., Haritani, M., Ouchi, Y., Sverlow, K. and Conrad, P. A. (1997). In vitro isolation and characterisation of a bovine Neospora species in Japan. Research in Veterinary Science 63, 77–80. doi:10.1016/S0034-5288(97)90162-4.CrossRefGoogle ScholarPubMed
Yamane, I., Shibahara, T., Kokuho, T., Shimura, K., Hamaoka, T., Haritani, M., Conrad, P. A., Park, C. H., Sawada, M. and Umemura, T. (1998). An improved isolation technique for bovine Neospora species. Journal of Veterinary Diagnostic Investigation 10, 364–368.CrossRefGoogle ScholarPubMed