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A larval paralysis assay for the detection of thiabendazole resistance in trichostrongyles

Published online by Cambridge University Press:  06 April 2009

I. A. Sutherland  and
D. L. Lee
I. A. Sutherland
Affiliation:
Department of Pure and Applied Biology, University of Leeds, Leeds LS2 9JT
D. L. Lee
Affiliation:
Department of Pure and Applied Biology, University of Leeds, Leeds LS2 9JT
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Summary

Infective-stage larvae of trichostrongyle nematodes, either resistant or susceptible to thiabendazole (TBZ), were incubated in eserine, an acetylcholinesterase inhibitor. Paralysis occurred in larvae treated with eserine but significant differences were observed in the percentage of larva immobilized between TBZ-resistant and TBZ-susceptible strains of the nematodes. These differences are probably related to the presence of higher levels of acetylcholinesterase in the TBZ-resistant strains than in the susceptible strains. This could be used as a rapid and inexpensive method of detecting resistance to TBZ in trichostrongyles.

Keywords

anthelmintic-resistancebenzimidazolesHaemonchus contortusnematodeOstertagia circumcinctaTrichostrongylus colubriformis
Type
Research Article
Information
Parasitology , Volume 100 , Issue 1 , February 1990 , pp. 131 - 135
Copyright
Copyright © Cambridge University Press 1990

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References

Albequerque, E. X., Idriss, M., Rao, K. S. & Arcava, Y. (1986). Sensitivity of nicotinic and glutamergic synapses to reversible and irreversible cholinesterase inhibitors. In Neuropharmacology and Pesticide Action (ed. Ford, M. G., Lunt, G. G., Reay, R. C. & Usherwood, P. N. R.), pp. 61–86. Chichester, England: Ellis Horwood Ltd and VCH Verlagsgesellschaft GmbH, Weinheim, FRD.Google Scholar
Baker, J. P. (1977). Assessment of the potential for and development of organophosphate resistance in field populations of Myzus persicae. Annals of Applied Biology 86, 1–9.CrossRefGoogle Scholar
Bennett, J. L. & Pax, R. A. (1986). Micromotility meter: an instrument designed to evaluate the action of drugs on motility of larval and adult nematodes. Parasitology 93, 341–6.CrossRefGoogle ScholarPubMed
Cawthorne, P. J. G. & Cheong, F. H. (1984). Prevalence of anthelmintic resistant nematodes in sheep in southeast England. Veterinary Record 114, 562–4.CrossRefGoogle Scholar
Coles, G. C. & Simpkin, K. G. (1977). Resistance of nematode eggs to the ovicidal activity of benzimidazoles. Research in Veterinary Science 22, 386–7.CrossRefGoogle Scholar
Coles, G. C., Tritschler, J. P., Laste, N. J. & Schmidt, A. L. (1988). Larval development test for detection of anthelmintic resistant nematodes. Research in Veterinary Science 45, 50–3.CrossRefGoogle ScholarPubMed
Folz, S. D., Pax, R.A., Thomas, E. M., Bennett, J. L., Lee, B. L. & Conder, G. A. (1987 a). Detecting in vitro anthelmintic effects with a micromotility meter. Veterinary Parasitology 24, 241–50.CrossRefGoogle ScholarPubMed
Folz, S. D., Pax, R. A., Thomas, E. M., Bennett, J. L., Lee, B. L. & Conder, G. A. (1987 b). Mortility response of benzimidazole-resistant Haemonchus contortus larvae to several anthelmintics. Proceedings of the Helminthological Society of Washington 54, 249–53.Google Scholar
Friedman, P. A. & Platzer, E. G. (1980). Interaction of anthelmintic benzimidazoles with Ascaris suum embryonic tubulin. Biochimica et Biophysica Acta 630, 271–8.CrossRefGoogle ScholarPubMed
Lacey, E. & Snowden, K. L. (1988). A routine diagnostic assay for the detection of benzimidazole resistance in parasitic nematodes using tritiated benzimidazole carbamates. Veterinary Parasitology 27, 309–24.CrossRefGoogle ScholarPubMed
Lee, D. L. & Atkinson, H. J. (1976). The Physiology of Nematodes 2nd Edn. London: Macmillan Press.CrossRefGoogle Scholar
Le Jambre, L. F. (1976). Egg hatch as an in vitro assay of thiabendazole resistance in nematodes. Veterinary Parasitology 2, 385–91.CrossRefGoogle Scholar
Le Jambre, L. F., Crofton, H. D. & Whitlock, J. H. (1970). An assay technique for nematode egg hatchability. Transactions of the American Microscopical Society 89, 397–406.CrossRefGoogle Scholar
Lewis, J. A., Wu, C.-H., Levine, J. H. & Berg, H. (1980). Levamisole-resistant mutants of the nematode Caenorhabditis elegans appear to lack pharmacological acetylcholine receptors. Neuroscience 5, 967–89.CrossRefGoogle ScholarPubMed
Martin, P. J. & Le Jambre, L. F. (1979). Larval paralysis test as an in vitro assay of levamisole and morantel tartrate resistance in Ostertagia. Veterinary Science Communications 3, 159–64.CrossRefGoogle Scholar
Sangster, N. C., Pritchard, R. K. & Lacey, E. (1985). Tubulin and benzimidazole resistance in Trichostrongylus colubriformis (Nematoda). Journal of Parasitology 71, 645–51.CrossRefGoogle ScholarPubMed
Sawicki, R. M., Devonshire, A. L., Rice, A. D., Moores, G. D., Petzing, S. M. & Cameron, A. (1978). The detection and distribution of organophosphate and carbamate insecticide resistant Myzus persicae in Britain in 1976. Pesticide Science 9, 189–201.CrossRefGoogle Scholar
Shaw, K. P., Arcava, Y., Akaike, A., Daly, J. W., Rickett, D. L. & Albequerque, E. X. (1985). The reversible cholinesterase inhibitor physostigmine has channel-blocking and agonist effects in the acetylcholine receptor-ion channel complex. Molecular Pharmacology 28, 527–38.Google ScholarPubMed
Sutherland, I. A. (1987). Anthelmintic resistance in nematodes. Ph.D. thesis, University of Leeds.Google Scholar
Sutherland, I. A., Lee, D. L. & Lewis, D. (1988). Detection of benzimidazole resistance in trichostrongylid nematodes. Parasitology Today 4, 22–4.CrossRefGoogle ScholarPubMed
Sutherland, I. A., Lee, D. L. & Lewis, D. (1989). Colorimetric assay for the detection of benzimidazole resistance in trichostrongyles. Research in Veterinary Science 46, 363–6.CrossRefGoogle ScholarPubMed
Waller, P. J. (1985). Resistance to anthelmintics and the implications for animal production. In Resistance in Nematodes to Anthelmintic Drugs (ed. Anderson, N. & Waller, P. J.), pp. 1–13. Glebe, NSW: CSIRO and Australian Wool Corporation.Google Scholar
Weston, K. M., O'brien, R. W. & Prichard, R. K. (1984). Respiratory metabolism and thiabendazole susceptibility in developing eggs of Haemonchus contortus. International Journal for Parasitology 14, 159–64.CrossRefGoogle ScholarPubMed
Whitlock, H. V., Kelly, J. D., Porter, C. J., Griffin, D. L. & Martin, I. C. A. (1980). In vitro screening for anthelmintic resistance in strongyles of sheep and horses. Veterinary Parasitology 7, 215–32.CrossRefGoogle Scholar