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MALDI-TOF MS in clinical parasitology: applications, constraints and prospects

Published online by Cambridge University Press:  08 July 2016

NEELJA SINGHAL ,
MANISH KUMAR  and
JUGSHARAN SINGH VIRDI
NEELJA SINGHAL
Affiliation:
Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi-110021, India
MANISH KUMAR
Affiliation:
Department of Biophysics, University of Delhi South Campus, Benito Juarez Road, New Delhi-110021, India
JUGSHARAN SINGH VIRDI*
Affiliation:
Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi-110021, India
*
*Corresponding author: Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi-110021, India. E-mail: virdi_dusc@rediffmail.com
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Summary

Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is currently being used for rapid and reproducible identification of bacteria, viruses and fungi in clinical microbiological laboratories. However, some studies have also reported the use of MALDI-TOF MS for identification of parasites, like Leishmania, Giardia, Cryptosporidium, Entamoeba, ticks and fleas. The present review collates all the information available on the use of this technique for parasites, in an effort to assess its applicability and the constraints for identification/diagnosis of parasites and diseases caused by them. Though MALDI-TOF MS-based identification of parasites is currently done by reference laboratories only, in future, this promising technology might surely replace/augment molecular methods in clinical parasitology laboratories.

Keywords

MALDI-TOFparasitesdiagnosisprotozoansticks
Type
Review Article
Information
Parasitology , Volume 143 , Issue 12 , October 2016 , pp. 1491 - 1500
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Copyright
Copyright © Cambridge University Press 2016 

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