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A new PCR-based approach for the specific amplification of DNA from different Schistosoma species applicable to human urine samples

Published online by Cambridge University Press:  12 July 2006

N. SANDOVAL ,
M. SILES-LUCAS ,
J. L. PÉREZ-ARELLANO ,
C. CARRANZA ,
S. PUENTE ,
J. LÓPEZ-ABÁN  and
A. MURO
N. SANDOVAL
Affiliation:
Laboratorio de Inmunología y Parasitología Molecular, CISET, Facultad de Farmacia, Universidad de Salamanca. Avda. Campo Charro, s/n. 37007-Salamanca, Spain
M. SILES-LUCAS
Affiliation:
Laboratorio de Inmunología y Parasitología Molecular, CISET, Facultad de Farmacia, Universidad de Salamanca. Avda. Campo Charro, s/n. 37007-Salamanca, Spain
J. L. PÉREZ-ARELLANO
Affiliation:
Departamento de Ciencias Médicas y Quirúrgicas. Facultad de Ciencias de la Salud. Universidad de Las Palmas de Gran Canaria. C/Doctor Pasteur, s/n. 35080-Las Palmas de Gran Canaria, Spain
C. CARRANZA
Affiliation:
Departamento de Ciencias Médicas y Quirúrgicas. Facultad de Ciencias de la Salud. Universidad de Las Palmas de Gran Canaria. C/Doctor Pasteur, s/n. 35080-Las Palmas de Gran Canaria, Spain
S. PUENTE
Affiliation:
Sección de Medicina Tropical, Servicio de Enfermedades Infecciosas, Hospital Carlos III. 28029-Madrid, Spain
J. LÓPEZ-ABÁN
Affiliation:
Laboratorio de Inmunología y Parasitología Molecular, CISET, Facultad de Farmacia, Universidad de Salamanca. Avda. Campo Charro, s/n. 37007-Salamanca, Spain
A. MURO
Affiliation:
Laboratorio de Inmunología y Parasitología Molecular, CISET, Facultad de Farmacia, Universidad de Salamanca. Avda. Campo Charro, s/n. 37007-Salamanca, Spain
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Abstract

Currently available methods for the diagnosis of human schistosomiasis often lack enough sensitivity and specificity. Recently, several authors have developed more specific and sensitive diagnostic methods, mainly based on the polymerase chain reaction (PCR) technique. Nevertheless, these have been only applied for the diagnosis of 1 out of 4 Schistosoma species affecting man (S. mansoni). Additionally, application of specific PCR has been exclusively used for blood or faecal patients' samples. Here, we develop a new, high sensitive PCR approach that allows the genus- and species-specific amplification of the main 4 Schistosoma species causing disease in man plus S. bovis. We further successfully apply this technique for the detection of parasite DNA in easy-to-handle urine samples from patients with schistosomiasis. With these samples, we have found 94·4% sensitivity and 99·9% specificity when applying a genus-specific (Schistosoma spp.) primer pair, and 100% sensitivity and 98·9% specificity in a species-specific (S. mansoni) PCR.

Keywords

Schistosoma sppdiagnosisurinePCR
Type
Research Article
Information
Parasitology , Volume 133 , Issue 5 , November 2006 , pp. 581 - 587
Copyright
2006 Cambridge University Press

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