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Phylogeny of diplozoids in five genera of the subfamily Diplozoinae Palombi, 1949 as inferred from ITS-2 rDNA sequences

Published online by Cambridge University Press:  14 December 2006

Q. GAO ,
M. X. CHEN ,
W. J. YAO ,
Y. GAO ,
Y. SONG ,
G. T. WANG ,
M. X. WANG  and
P. NIE
Q. GAO
Affiliation:
State Key Laboratory of Freshwater Ecology and Biotechnology, and Laboratory of Fish Diseases, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, 430072, China
M. X. CHEN
Affiliation:
State Key Laboratory of Freshwater Ecology and Biotechnology, and Laboratory of Fish Diseases, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, 430072, China College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei Province, 430070, China
W. J. YAO
Affiliation:
State Key Laboratory of Freshwater Ecology and Biotechnology, and Laboratory of Fish Diseases, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, 430072, China
Y. GAO
Affiliation:
State Key Laboratory of Freshwater Ecology and Biotechnology, and Laboratory of Fish Diseases, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, 430072, China
Y. SONG
Affiliation:
State Key Laboratory of Freshwater Ecology and Biotechnology, and Laboratory of Fish Diseases, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, 430072, China
G. T. WANG
Affiliation:
State Key Laboratory of Freshwater Ecology and Biotechnology, and Laboratory of Fish Diseases, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, 430072, China
M. X. WANG
Affiliation:
College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei Province, 430070, China
P. NIE*
Affiliation:
State Key Laboratory of Freshwater Ecology and Biotechnology, and Laboratory of Fish Diseases, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, 430072, China
*
*Corresponding author: Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, 430072, China. Tel: +86 27 68780736. Fax: +86 27 68780123. E-mail: pinnie@ihb.ac.cn
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Summary

The phylogenetic relationship of 5 genera, i.e. Diplozoon Nordmann, 1832, ParadiplozoonAchmerov, 1974, InustiatusKhotenovsky, 1978, SindiplozoonKhotenovsky, 1981, and EudiplozoonKhotenovsky, 1985 in the subfamily Diplozoinae Palombi, 1949 (Monogenea, Polyopisthocotylea) was inferred from rDNA ITS-2 region using neighbour-joining (NJ), maximum likelihood (ML) and Bayesian methods. The phylogenetic trees produced by using NJ, ML and Bayesian methods exhibit essentially the same topology. Surprisingly, freshwater species of Paradiplozoon from Europe clustered together with species of Diplozoon, but separated from Chinese Paradiplozoon species. The results of molecular phylogeny and lower level of divergence (4·1–15·7%) in ITS-2 rDNA among Paradiplozoon from Europe and Diplozoon and, on the other hand, high level of divergence (45·3–53·7%) among Paradiplozoon species from Europe and China might indicate the non-monophyletic origin of the genus Paradiplozoon. Also, the generic status of European Paradiplozoon needs to be revised. The species of Paradiplozoon in China is a basal group in Diplozoinae as revealed by NJ and Bayesian methods, and Sindiplozoon appears to be closely related to European Paradiplozoon and Diplozoon with their relationship to Eudiplozoon and Inustiatus being unresolved.

Keywords

diplozoidDiplozoinaeITS-2rDNAphylogeny
Type
Research Article
Information
Parasitology , Volume 134 , Issue 5 , May 2006 , pp. 695 - 703
Copyright
Copyright © Cambridge University Press 2006

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References

REFERENCES

Achmerov, A. C. (1974). The new species of diplozoons from the Amur River. Trudy Gelmintologicheskoi Laboratorii Academii Nauk, SSSR 24, 5–19. (In Russian.)Google Scholar
Bachellerie, J.-P. and Qu, L.-H. (1993). Ribosomal RNA probes for detection and identification of species. In Protocols in Molecular Parasitology (ed. Hyde, J. E.), pp. 249–263. Humana Press, Totowa, New Jersey.CrossRefGoogle Scholar
Denis, A., Gabrion, C. and Lambert, A. (1983). Présence en France de deux parasites d'origine est-asiatique: Diplozoon nipponicum Goto, 1891 (Monogenea) et Bothriocephalus acheilognathi Yamaguti, 1934 (Cestoda) chez Cyprinus carpio (Teleostei, Cyprinidae). Bulletin Français de Pisciculture 289, 128–134.Google Scholar
Felsenstein, J. (1991). PHYLIP – Phylogeny Inference Package, Version 3.4. University of Washington, Seattle, Washington.Google Scholar
Galtier, N., Gouy, M. and Gautier, C. (1996). Seaview and Phylowin: two graphic tools for sequence alignment and molecular phylogeny. Cabios 12, 543–548.Google ScholarPubMed
Gelnar, M., Šebelová, Š., Dušek, L., Koubková, B., Jurajda, P. and Zahrádková, S. (1997). Biodiversity of parasites in freshwater environment in relation to pollution. I. Species richness and heterogeneity measures. Parassitologia 39, 89–99.Google Scholar
Gläser, H.-J. and Gläser, B. (1964). Zur Taxonomie der Gattung Diplozoon Nordmann, 1832. Zeitschrift für Parasitenkunde 25, 164–192.CrossRefGoogle Scholar
Gu, X., Fu, Y. X. and Li, W. H. (1995). Maximum likelihood estimation of the heterogeneity of substitution rate among nucleotide sites. Molecular Biology and Evolution 12, 546–557.Google ScholarPubMed
Hoole, D. (1994). Tapeworm infections in fish: past and future problems. In Parasitic Diseases of Fish (ed. Pike, A. W. and Lewis, J. W.), pp. 119–140. Samara Publishing Limited, Dyfed, UK.Google Scholar
Huelsenbeck, J. P. and Ronquist, F. (2001). MrBayes: Bayesian inference of phylogeny. Bioinformatics 17, 754–755.Google Scholar
Khotenovsky, I. A. (1978). A new genus of monogeneans of the family Diplozoidae Palombi, 1949, (Monogenoidea). Parazitologyia 12, 543–547. (In Russian.)Google Scholar
Khotenovsky, I. A. (1981). Systematics and phylogeny of the families Diplozoidae and Discocotylidae (Monogenea). Parazitologyia 30, 166–175. (In Russian.)Google Scholar
Khotenovsky, I. A. (1985). Fauna of the URSS Monogenea. Nauka, Leningrad. (In Russian.)Google Scholar
Kumar, S., Tamura, K., Jakobsen, I. B. and Nei, M. (2001). MEGA 2: Molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.CrossRefGoogle ScholarPubMed
Lambert, A. and Le Brun, N. (1988). Hyphothèse sur l'origine biogéographique de Diplozoon (Monogenea, Polyopisthocotylea). Annales de Parasitologie Humaine et Comparée 63, 99–102.Google Scholar
Le Brun, N., Renaud, F. and Lambert, A. (1988). The genus Diplozoon (Monogenea, Polyopisthocotylea) in southern France: speciation and specificity. International Journal for Parasitology 18, 395–400.CrossRefGoogle Scholar
Matejusová, I., Koubková, B., D'amelio, S. and Cunningham, C. O. (2001). Genetic characterization of six species of diplozoids. Parasitology 123, 465–474.Google Scholar
Matejusová, I., Koubková, B., Gelnar, M. and Cunningham, C. O. (2002). Paradiplozoon homoion Bychowsky & Nagibina, 1959 versus P. gracile Reichenbach-Klinke, 1961 (Monogenea): two species or phenotypic plasticity? Systematic Parasitology 53, 39–47.CrossRefGoogle ScholarPubMed
Matejusová, I., Koubková, B. and Cunningham, C. O. (2004). Identification of European diplozoids (Monogenea, Diplozoinae) by restriction digestion of the ribosomal RNA internal transcribed spacer. Journal of Parasitology 90, 817–822.CrossRefGoogle ScholarPubMed
Moravec, F., Gelnar, M., Ergens, R. and Scholz, T. (1997). Metazoan parasites of fishes from the section of the Vltava River supposed to be affected by operation of the Temelin nuclear electric powerstation, Czech Republic. Acta Societatis Zoologicae Bohemica 61, 65–76.Google Scholar
Nelson, J. S. (1994). Fishes of the World (3rd Edn). John Wiley and Sons, New York.Google Scholar
Nie, P., Yao, W. J., Gao, Q., Wang, G. T. and Zhang, Y. A. (1999). Diversity of intestinal helminth communities of carp, Cyprinus carpio L. from six lakes in the flood plain of the Yangtze River in China. Journal of Fish Biology 54, 171–180.Google Scholar
Pan, J. H., Zhang, J. Y. and Li, Z. C. (1990). Fish Parasitology. Science Press, Beijing. (In Chinese.)Google Scholar
Page, R. D. M. (2001). TreeView, Version 1.6.6. Distribution by the author at http://taxonomy.zoology.gla.ac.uk/rod/rod.html.Google Scholar
Posada, D. and Crandall, K. A. (1998). Model test: testing the model of DNA substitution. Bioinformatics 14, 817–818.CrossRefGoogle Scholar
Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Molecular Cloning: a Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.Google Scholar
Shimodaira, H. and Hasegawa, M. (1999). Multiple comparisons of loglikelihoods with applications to phylogenetic inference. Molecular Biology and Evolution 16, 1114–1116.CrossRefGoogle Scholar
Sicard, M., Desmarais, E. and Lambert, A. (2001). Molecular characterization of Diplozoidae populations on five Cyprinidae species: consequences for host specificity. Comptes rendus de l'Académie des Sciences, Serie 3 Life Sciences 324, 709–717.Google Scholar
Swofford, D. L. (2003). PAUP*. Phylogenetic Analysis using Parsimony (*and other Methods). Version 4. Sinauer, Sunderland, Massachusetts.Google Scholar
Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. and Higgins, D. G. (1997). CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 24, 4876–4882.Google Scholar
Wu, B. H., Long, S. and Wang, W. J. (2000). Fauna Sinica: Platyhelminthes: Monogenea. Science Press, Beijing.Google Scholar
Xia, X. and Xie, Z. (2001). DAMBE: Data analysis in molecular biology and evolution. Journal of Heredity 92, 371–373.CrossRefGoogle ScholarPubMed