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The source and nature of some functional antigens of Trichuris muris

Published online by Cambridge University Press:  06 April 2009

Simon N. Jenkins  and
D. Wakelin
Simon N. Jenkins
Affiliation:
Wllcome Laboratories for Experimental Parasitology, University of Glasgow, Bearsden Road, Bearsden, Glasgow, G61 1QH
D. Wakelin
Affiliation:
Wllcome Laboratories for Experimental Parasitology, University of Glasgow, Bearsden Road, Bearsden, Glasgow, G61 1QH
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Extract

Vaccination with whole male worm extract (AMA), stichoseome extract (SA) and a short-term inculbation fluid (EXA) of adult Trichuris muris induced a high degree of protective immunity in mice as assessed by reduction in larval worm burden.

Immunodiffusion of AMA against rabbit anti-AMA serum revealed five precipitin lines, one of which was common to both EXA and SA with which only single lines were obtained.

Physico-chemical tests suggested that the antigen is a protein and that carbohydrate, lipid and DNA do not contribute to its antigenicity. Partial fractionation was obtained with ammonium sulphate. All preparations with immunogenic activity showed the common line of EXA on immunodiffusion.

It is concluded that one of the protective immunogens is a protein which can be associated with the precipitin line and originates in the stichosome.

Type
Research Article
Information
Parasitology , Volume 74 , Issue 2 , April 1977 , pp. 153 - 161
Copyright
Copyright © Cambridge University Press 1977

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