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Ultrastructural evidence for complement and antibody-dependent damage to schistosomula of Schistosoma mansoni by rat eosinophils in vitro

Published online by Cambridge University Press:  06 April 2009

Diane J. McLaren ,
F. J. Ramalho-Pinto  and
S. R. Smithers
Diane J. McLaren
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA
F. J. Ramalho-Pinto
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA
S. R. Smithers
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA
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Summary

Rat peritoneal eosinophils adhere to live Schistosoma mansoni schistosomula in vitro in the presence of fresh normal rat serum, or in heat-inactivated serum from rats immune to the parasite. When the eosinophils are present in sufficient numbers the worms show ultrastructural evidence of surface damage and are ultimately killed. It is believed that the appearance of focal lesions in the tegument of the schistosomulum follows the secretion of enzymes by the eosinophils onto the parasite surface. The cells have been observed within these lesions and later between the basal plasma membrane of the tegument and the underlying interstitial material. It is suggested that the cells are responsible for prising the tegument away from the body of the worm. The detached tegument shows evidence of further degradation. Adherent eosinophils which have released their secretions appear to degenerate and are eventually replaced by macrophages. Remnants of both the expended eosinophils and the disrupted tegument have been identified within the macrophages. Adherence of eosinophils through C3–C3 receptor interaction results in earlier and more severe damage to the schistosomula than when adherence occurs through Fe receptors. Rat eosinophils also adhere to C3-coated, glutaraldehyde-flxed schistosomula and C3-coated Sepharose beads. However, evidence of enzyme secretion is only obtained when the target is a schistosomulum.

Type
Research Article
Information
Parasitology , Volume 77 , Issue 3 , December 1978 , pp. 313 - 324
Copyright
Copyright © Cambridge University Press 1978

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References

REFERENCES

Butterworth, A. E., Sturrock, R. F., Houba, V., Mahmoud, A. A. F., Sher, A. & Rees, P. H. (1975). Eosinophils as mediators of antibody-dependent damage to schistosomula, Nature, London 256, 727–9.CrossRefGoogle ScholarPubMed
Butterworth, A. E., David, J. R., Franks, D., Mahmoud, A. A. F., David, P. H., Sturrock, R. F. & Houba, V. (1977). Antibody-dependent eosinophil-mediated damage to [51Cr]labelled schistosomula of Schistosoma mansoni. I. Damage by purified eosinophils. Journal of Experimental Medicine 145, 136–50.CrossRefGoogle Scholar
Hsü, S. Y. L., Hsü, H. F., Penick, G. D., Lust, G. L., Osborne, J. W. & Cheng, H. F. (1975). Mechanism of immunity to schistosomiasis: histopathological study of lesions elicited in Rhesus monkeys during immunizations and challenge with cercariae of Schistosoma japonicum. Journal of the Reticuloendothelial Society 18, 167–85.Google ScholarPubMed
von Lichtenberg, F., Sher, A., Gibbons, N. & Doughty, B. L. (1976). Eosinophil-enriched inflammatory response to schistosomula in the skin of mice immune to Schistosoma mansoni. American Journal of Pathology 84, 479–500.Google Scholar
Mackenzie, C. D., Ramalho-Pinto, F. J., McLaren, D. J. & Smithers, S. R. (1977). Antibody-mediated adherence of rat eosinophils to schistosomula of Schistosoma mansoni in vitro. Clinical and Experimental Immunology 30, 97–104.Google ScholarPubMed
Mahmoud, A. A. F., Warren, K. S. & Peters, P. A. (1975). A role for the eosinophil in acquired resistance to Schistosoma mansoni infection as determined by anti-eosinophil serum. Journal of Experimental Medicine 142, 805–13.CrossRefGoogle Scholar
McLaren, D. J. (1974). The anterior glands of adult Necator americanus (Nematoda: Strongyloidea). I. Ultrastructural studies. International Journal for Parasitology 4, 25–38.CrossRefGoogle ScholarPubMed
McLaren, D. J., Mackenzie, C. D. & Ramalho-Pinto, F. J. (1977). Ultrastructural observations on the in vitro interaction between rat eosinophils and some parasitic helminths (Schistosoma mansoni, Trichinella spiralis and Nippostrongylus brasiliensis). Clinical and Experimental Immunology 30, 105–18.Google ScholarPubMed
Ramalho-Pinto, F. J., Gazzinelli, G., Howells, R. E., Mota-Santos, T. A., Figueiredo, E. A. & Pellegrino, J. (1974). Schistosoma mansoni: defined system for stepwise transformation of cercariae to schistosomula in vitro. Experimental Parasitology 36, 360–72.CrossRefGoogle Scholar
Ramalho-Pinto, F. J., McLaren, D. J. & Smithers, S. R. (1978). Complement-mediated killing of schistosomula of Schistosoma mansoni by rat eosinophils in vitro. Journal of Experimental Medicine 147, 147–56.CrossRefGoogle ScholarPubMed
Smithers, S. R. & Terry, R. J. (1965). The infection of laboratory hosts with cercariae of Schistosoma mansoni and the recovery of adult worms. Parasitology 55, 695–700.CrossRefGoogle ScholarPubMed
Takenaka, T., Okuda, M., Kawabori, S. & Kubo, K. (1977). Extracellular release of peroxidase from eosinophils by interaction with immune complexes. Clinical and Experimental Immunology 28, 56–60.Google ScholarPubMed