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Biochemical and morphological pathology of the foot of the schistosome vector Biomphalaria glabrata infected with Schistosoma mansoni

Published online by Cambridge University Press:  06 April 2009

S. N. Thompson ,
R. W. K. Lee ,
V. Mejia-Scales  and
M. Shams El-Din
S. N. Thompson
Affiliation:
Departments of Entomology and Chemistry, University of California, Riverside, California 92521, USA
R. W. K. Lee
Affiliation:
Departments of Entomology and Chemistry, University of California, Riverside, California 92521, USA
V. Mejia-Scales
Affiliation:
Departments of Entomology and Chemistry, University of California, Riverside, California 92521, USA
M. Shams El-Din
Affiliation:
Departments of Entomology and Chemistry, University of California, Riverside, California 92521, USA
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Summary

Infection by Schistosoma mansoni resulted in morphological and biochemical changes to the foot of its intermediate host, Biomphalaria glabrata. Migration through, and emergence of cercariae from, the foot was observed and evidenced by lesions on the ciliated foot surface. This was accompanied by a significant decrease in the velocity of movement by infected individuals. In vivo31P NMR spectral analyses demonstrated that the foot of infected snails had a lower phosphoarginine (PA)/adenosine 5' triphosphate (ATP) ratio than that of uninfected controls. Moreover, kinetic experiments, employing saturation transfer, demonstrated the pseudo-first-order rate constant for the arginine kinase-catalysed exchange reaction in the forward direction, that is, PA→ATP was decreased by infection. The reverse reaction was not observed by the NMR methods used. PA was depleted upon exposure to hypoxic conditions suggesting its traditional role in preserving ATP level. Partly oxidized metabolic end-products were not observed in snails maintained under aerobic conditions, but succinate, propionate, acetate and lactate rapidly accumulated under hypoxic conditions.

Keywords

Schistosoma mansoniBiomphalaria glabratafoot pathologyNMR analysis
Type
Research Article
Information
Parasitology , Volume 107 , Issue 3 , September 1993 , pp. 275 - 285
Copyright
Copyright © Cambridge University Press 1993

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