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Immunization of mice with gamma-irradiated intramuscularly injected schistosomula of Schistosoma mansoni

Published online by Cambridge University Press:  06 April 2009

Q. D. Bickle ,
M. G. Taylor ,
M. J. Doenhoff  and
G. S. Nelson
Q. D. Bickle
Affiliation:
Winches Farm Field Station, London School of Hygiene and Tropical Medicine, 395 Hatfield Road, St Albans, Herts. AL4 0XQ
M. G. Taylor
Affiliation:
Winches Farm Field Station, London School of Hygiene and Tropical Medicine, 395 Hatfield Road, St Albans, Herts. AL4 0XQ
M. J. Doenhoff
Affiliation:
Winches Farm Field Station, London School of Hygiene and Tropical Medicine, 395 Hatfield Road, St Albans, Herts. AL4 0XQ
G. S. Nelson
Affiliation:
Winches Farm Field Station, London School of Hygiene and Tropical Medicine, 395 Hatfield Road, St Albans, Herts. AL4 0XQ
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Summary

The parameters involved in the induction of resistance against Schistosoma mansoni by injection of irradiated, artificially transformed schistosomula were studied in mice. Single intramuscular injections of 500 schistosomula exposed to radiation doses in the range 2·3 to 160 krad. resulted in significant protection (in the range 20 to 50% as assessed by reduced worm burdens) against a challenge infection administered at intervals from 3 to 24 weeks post-vaccination. However, schistosomula irradiated with 20 krad. consistently resulted in better protection than those exposed to either higher or lower radiation doses despite the persistence of stunted adults from the infections irradiated with 2·3 krad. Vaccination with 40 krad. schistosomula resulted in significant protection in terms of reduced worm and tissue egg burdens and increased survival following lethal challenge. Varying the number of irradiated schistosomula, the frequency and route of their administration, the site of challenge and the strain of host all failed to enhance the level of resistance. However, percutaneously applied, irradiated cercariae were found to be more effective in stimulating resistance (60%) than intramuscularly injected, irradiated schistosomula (40%).

Type
Research Article
Information
Parasitology , Volume 79 , Issue 2 , October 1979 , pp. 209 - 222
Copyright
Copyright © Cambridge University Press 1979

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References

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