Hostname: page-component-cd9895bd7-jn8rn Total loading time: 0 Render date: 2024-12-22T17:12:34.190Z Has data issue: false hasContentIssue false
  • English
  • Français

Interactions between the plasma proteins of Biomphalaria glabrata (Gastropoda) and the sporocyst tegument of Schistosoma mansoni (Trematoda)

Published online by Cambridge University Press:  06 April 2009

C. J. Bayne ,
E. S. Loker  and
Mary A. Yui
C. J. Bayne
Affiliation:
Department of Zoology, Oregon State University, Corvallis, Oregon 97331
E. S. Loker
Affiliation:
Department of Zoology, Oregon State University, Corvallis, Oregon 97331
Mary A. Yui
Affiliation:
Department of Zoology, Oregon State University, Corvallis, Oregon 97331
Get access Rights & Permissions [Opens in a new window]

Summary

The tegumental surface of Schistosoma mansoni sporocysts is the site of both nutritive and immunological interactions with haemolymph cells and plasma of Biomphalaria glabrata, the schistosome intermediate host. Within minutes of being placed in host plasma, sporocysts acquire plasma antigens, and within 3 h host plasma antigens are present on the surface at near steady state. Though a wide variety of peptides is acquired, there is selection. Furthermore, some differences occur in the peptides acquired from the plasma of susceptible and resistant strains of snail. Acquired antigens are rapidly processed, and are predominantly undetectable in tegumental extracts after a few hours. In contrast, rabbit antibody on sporocysts remains in situ for at least 48 h, so under some conditions there is stable expression of certain tegumental antigenic determinants.These data, obtained using antibodies to snail plasma antigens and to sporocyst tegumental antigens, are discussed in the light of current ideas on the cellular and molecular basis of susceptibility and resistance in this host#parasite system.

Type
Research Article
Information
Parasitology , Volume 92 , Issue 3 , June 1986 , pp. 653 - 664
Copyright
Copyright © Cambridge University Press 1986

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Basch, P. F. (1976). Intermediate host specificity in Schistosoma mansoni. Experimental Parasitology 39, 150–69.CrossRefGoogle ScholarPubMed
Bayne, C. J. (1980). Humoral factors in molluscan parasite immunity. In Aspects of Developmental and Comparative Immunology, vol. 1 (ed. Solomon, J. B.), pp. 113–24. Oxford and New York: Pergamon.Google Scholar
Bayne, C. J., Buckley, P. M. & Dewan, P. C. (1980 a). Macrophage-like hemocytes of resistant Biomphalaria glabrata are cytotoxic for sporocysts of Schistosonia manaoni in vitro. Journal of Parasitology 66, 413–19.CrossRefGoogle Scholar
Bayne, C. J., Buckley, P. M. & Dewan, P. C. (1980b). Schistosoma mansoni: cytotoxicity of hemocytes from susceptible snail hosts for sporocysts in plasma from resistant Bioniphalaria glabrata. Experimental Parasitology 50, 409–16.CrossRefGoogle Scholar
Bayne, C. J. & Stephens, J. A. (1983). Schistosoma mansoni and Biomphalaria glabrata share epitopes: antibodies to sporocysts bind host snail hemocytes. Journal of Invertebrate Pathology 42, 221–3.CrossRefGoogle ScholarPubMed
Bayne, C. J., Loker, E. S., Yui, M. A. & Stephens, J. A. (1984). Immune recognition of Schistosoma mansoni primary sporocysts may require specific receptors on Biomphalaria glabrata hemocytes. Parasite Immunology 6, 519–28.CrossRefGoogle ScholarPubMed
Capron, A., Auriault, C., Mazingue, C., Capron, M. & Torpier, G. (1980). In The Host–Invader Interplay (ed. Bosch, H. van den), p. 217. Amsterdam: Elsevier/North Holland.Google Scholar
Chernin, E. (1963). Observations on hearts transplanted in vitro from the snail Australorbis glabratus. Journal of Parasitology 49, 353–64.CrossRefGoogle Scholar
Granath, W. O. & Yoshino, T. P. (1984). Schistosoma mansoni: Passive transfer of resistance by serum in the vector snail, Biomphalaria glabrata. Experimental Parasitology 58, 188–93.CrossRefGoogle ScholarPubMed
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227, 680–4.CrossRefGoogle ScholarPubMed
Lie, K. J., Jeong, K. J. & Heyneman, P. (1980). Tissue reactions induced by Schistosoma mansoni in Biomphalaria glabrata. Annals of Tropical Medicine and Parasitology 74, 157–66.CrossRefGoogle ScholarPubMed
Loker, E. S. & Bayne, C. J. (1982). In vitro encounters between Schistosoma mansoni primary sporocysts and hemolymph components of susceptible and resistant strains of Biomphalaria glabrata. American Journal of Tropical Medicine and Hygiene 31, 999–1005.CrossRefGoogle ScholarPubMed
Loker, E. S., Bayne, C. J., Buckley, P. M. & Kruse, K. T. (1982). Ultrastructure of encapsulation of Schistosoma mansoni mother sporocysts by hemocytes of juveniles of the 10-R2 strain of Biomphalaria glabrata. Journal of Parasitology 68, 84–94.CrossRefGoogle ScholarPubMed
Loker, IS., Yui, M. A. & Bayne, C. J. (1984). Schistosoma mansoni: agglutination of sporocysts, and formation of gels on miracidia transforming in plasma of Biomphalaria glabrata. Experimental Parasitology 58, 56–62.CrossRefGoogle ScholarPubMed
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265–75.CrossRefGoogle ScholarPubMed
McLaren, D. J. & Terry, R. J. (1982). The protective role of acquired host antigens during schistosome maturation. Parasite Immunology 4, 129–35.CrossRefGoogle ScholarPubMed
Merril, C. R., Goldman, P., Sedman, S. A. & Ebert, M. H. (1981). Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins. Science 211, 1437–9.CrossRefGoogle ScholarPubMed
Newton, W. L. (1952). The comparative tissue reaction for two strains of Australorbis glabratus to infection with Schistosoma mansoni. Journal of Parasitology 38, 362–6.CrossRefGoogle Scholar
Pan, S. C. T. (1980). The fine structure of the miracidium of Schistosoma mansoni. Journal of invertebrate Pathology 36, 307–72.CrossRefGoogle ScholarPubMed
Richards, C. S. (1975). Genetic factors in susceptibility of Bioniphalaria glabrata for different strains of Schistosoma mansoni. Parasitology 70, 231–41.CrossRefGoogle Scholar
Richards, C. S. (1984). Influence of snail age on genetic variations in susceptibility of Biomphalaria glabrata for infection with Schistosoma mansoni. Malacologia 25, 493–502.Google Scholar
Stibbs, H. H., Owczarzak, O., Bayne, C. J. & Dewan, P. C. (1979). Schistosome sporocyst-killing amoebae isolated from Biomphalaria glabrata. Journal of invertebrate Pathology 33, 159–70.CrossRefGoogle ScholarPubMed
Ulmer, M. J. (1970). Notes for the rearing of snails in the laboratory. In Experiments and Techniques in Parasitology (ed. Maclnnis, A. J. and Voge, M.), pp. 143–4. San Francisco: Freeman.Google Scholar
Yoshino, T. P. (1984). Selective acquisition of snail host antigens by larval Schistosoma mansoni: detection by monoclonal antibodies to snail hemocytes and hemolymph. In Aspects of Developmental and Comparative Immunology vol. 2 (ed. Cooper, E. L. and Wright, R. K.), p. 225 (abstract). New York: Pergamon.Google Scholar
Yoshino, T. P. & Bayne, C. J. (1983). Mimicry of snail host antigens by miracidia and primary sporocysts of Schistosoma mansoni. Parasite Immunology 5, 317–28.CrossRefGoogle ScholarPubMed