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Microarrays: new tools to unravel parasite transcriptomes

Published online by Cambridge University Press:  01 June 2005

G. N. GOBERT ,
L. P. MOERTEL  and
D. P. McMANUS
G. N. GOBERT
Affiliation:
Molecular Parasitology Laboratory, Australian Centre for International and Tropical Health and Nutrition, Queensland Institute of Medical Research, 300 Herston Road, Brisbane, QLD 4029 Australia
L. P. MOERTEL
Affiliation:
Molecular Parasitology Laboratory, Australian Centre for International and Tropical Health and Nutrition, Queensland Institute of Medical Research, 300 Herston Road, Brisbane, QLD 4029 Australia The School of Chemical and Biomedical Sciences, Central Queensland University, Bruce Hwy, North Rockhampton, QLD 4702 Australia
D. P. McMANUS
Affiliation:
Molecular Parasitology Laboratory, Australian Centre for International and Tropical Health and Nutrition, Queensland Institute of Medical Research, 300 Herston Road, Brisbane, QLD 4029 Australia
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Abstract

The ability to monitor the expression levels of thousands of genes in a single microarray experiment is a huge progression from conventional Northern blot analysis or PCR-based techniques. Microarrays can play a pivotal role in the mass screening of genes in a wide range of fields including parasitology. The relatively few parasites that can be readily cultured or isolated from a host, as compared with cell lines or tissue sources, makes microarray technology ideal for maximizing experimental results from a limiting source of starting material. Khan et al. (1999a) commented in an early review of microarray technology “With this system in place, one can anticipate a time when data from thousands of gene expression experiments will be available for meta-analysis…..… leading to more robust results and subtle conclusions”. Now in 2005, microarrays represent a very powerful resource that can play an important role in the characterization and annotation of the transcriptomes of many parasites of medical and veterinary importance.

Keywords

microarraygene expressiongenomic sequencingtranscriptome
Type
Review Article
Information
Parasitology , Volume 131 , Issue 4 , October 2005 , pp. 439 - 448
Copyright
© 2005 Cambridge University Press

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