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A minimal medium for the growth of Plasmodium knowlesi in dilution cultures

Published online by Cambridge University Press:  06 April 2009

P. I. Trigg  and
W. E. Gutteridge
P. I. Trigg
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London, N.W.7
W. E. Gutteridge
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London, N.W.7
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Extract

A dilution medium for the cultivation in vitro of the erythrocytic stages of P. knowlesi has been simplified to contain the following: a balanced salt solution, glucose, isoleucine, methionine, adenosine, p-aminobenzoic acid, biotin and calcium pantothenate. The medium, in the presence of plasma gave better results than the Harvard medium for the growth of P. knowlesi during one asexual cycle and also allowed the subculture of the parasites through three asexual cycles before a reduction in the number of parasites was observed.

We should like to thank Dr F. Hawking and Dr K. N. Brown for their advice and Mr T. J. Scott-Finnigan for technical assistance. One of us (P.I.T.) received financial assistance from the World Health Organization.

Type
Research Article
Information
Parasitology , Volume 62 , Issue 1 , February 1971 , pp. 113 - 123
Copyright
Copyright © Cambridge University Press 1971

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