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Molecular determination of the origin of acephalic cysticercus

Published online by Cambridge University Press:  25 November 2004

J.-Y. CHUNG ,
W.-G. KHO ,
S.-Y. HWANG ,
E.-Y. JE ,
Y.-T. CHUNG ,
T.-S. KIM ,
K.-S. EOM ,
W.-M. SOHN ,
S.-Y. CHO  and
Y. KONG
J.-Y. CHUNG
Affiliation:
Department of Parasitology, College of Medicine and Department of Malariology, Paik Institute for Clinical Research, Inje University, Busan 614-735, Korea
W.-G. KHO
Affiliation:
Department of Parasitology, College of Medicine and Department of Malariology, Paik Institute for Clinical Research, Inje University, Busan 614-735, Korea
S.-Y. HWANG
Affiliation:
Department of Parasitology, College of Medicine and Department of Malariology, Paik Institute for Clinical Research, Inje University, Busan 614-735, Korea
E.-Y. JE
Affiliation:
Department of Molecular Parasitology and Center for Molecular Medicine, Sungkyunkwan University School of Medicine and Samsung Biomedical Research Institute, Suwon, 440-746, Korea
Y.-T. CHUNG
Affiliation:
Department of Neurosurgery, Inje University College of Medicine, Busan 614-735, Korea
T.-S. KIM
Affiliation:
Department of Tropical and Endemic Parasitic Diseases, National Institute of Health, Seoul 122-701, Korea
K.-S. EOM
Affiliation:
Department of Parasitology, Chungbuk National University College of Medicine, Chongju 360-763, Korea
W.-M. SOHN
Affiliation:
Department of Parasitology and Institute of Health Sciences, Gyeongsang National University College of Medicine, Jinju 660-751, Korea
S.-Y. CHO
Affiliation:
Department of Molecular Parasitology and Center for Molecular Medicine, Sungkyunkwan University School of Medicine and Samsung Biomedical Research Institute, Suwon, 440-746, Korea
Y. KONG
Affiliation:
Department of Molecular Parasitology and Center for Molecular Medicine, Sungkyunkwan University School of Medicine and Samsung Biomedical Research Institute, Suwon, 440-746, Korea
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Abstract

Acephalic cysticercus (Ac), a rarely developed multilobulated and nonencysted form of larval Taenia, causes hydrocephalus or adhesive arachnoiditis in the ventricles and subarachnoidal space that often lead to fatal outcome in affected patients. Ac has been proposed to originate from T. solium on the basis of morphological features, while no molecular data supporting the presumption have been available. In the present study, we investigated the immunological properties as well as molecular characteristics of Ac that was obtained surgically from 6 patients. Immunoblotting of the cyst fluid from Ac samples demonstrated the constitutive expression of a T. solium metacestode (TsM) 10 kDa protein. Specific antibodies against the truncated 10 kDa protein, which appears to be species specific for TsM cysticercosis, were detected in both serum and cerebrospinal fluid samples of Ac patients. Nucleotide sequences of mitochondrial cytochrome c oxidase subunit I (COI) and NADH dehydrogenase subunit 1 (ND1) genes of Ac were almost identical to those of T. solium but differed substantially from those of the other Taenia species. In phylogenetic analysis, Ac clustered with T. solium in a well-supported clade. Our results strongly suggest that Ac may have originated from T. solium.

Keywords

acephalic cysticercusneurocysticercosis10 kDa proteincytochrome c oxidase INADH dehydrogenase subunit 1
Type
Research Article
Information
Parasitology , Volume 130 , Issue 2 , February 2005 , pp. 239 - 246
Copyright
© 2005 Cambridge University Press

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References

REFERENCES

BOWLES, J., BLAIR, D. & McMANUS, D. P. ( 1992). Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Molecular and Biochemical Parasitology 54, 165–170.CrossRefGoogle Scholar
BOWLES, J. & McMANUS, D. P. ( 1994). Genetic characterization of the Asian Taenia, a newly described taeniid cestode of humans. American Journal of Tropical Medicine and Hygiene 50, 33–44.CrossRefGoogle Scholar
CHUNG, J. Y., BAHK, Y. Y., HUH, S., KANG, S. Y., KONG, Y. & CHO, S. Y. ( 1999). A recombinant 10-kDa protein of Taenia solium metacestodes specific to active neurocysticercosis. Journal of Infectious Diseases 180, 1307–1315.CrossRefGoogle Scholar
CHUNG, J. Y., YUN, D. H., EOM, K. S., KANG, S. Y., KONG, Y. & CHO, S. Y. ( 2002). Taenia solium: identification of specific antibody binding regions of metacestode 10-kDa protein. Experimental Parasitology 100, 87–94.CrossRefGoogle Scholar
DE-QUEIROZ, A. & ALKIRE, N. L. ( 1998). The phylogenetic placement of Taenia cestodes that parasitize humans. Journal of Parasitology 84, 379–383.CrossRefGoogle Scholar
DEL BRUTTO, O. H. ( 2002). Meningeal cysticercosis. In Taenia solium Cysticercosis: From Basic to Clinical Science ( ed. Singh, G. & Prabhakar, S.), pp. 177–197. CABI Publishing, Oxon.CrossRef
DEL BRUTTO, O. H., GARCIA, E., TALAMAS, O. & SOTELO, J. ( 1988). Sex related severity of inflammation in parenchymal brain cysticercosis. Archives of Internal Medicine 148, 544–546.CrossRefGoogle Scholar
DEL BRUTTO, O. H., SOTELO, J. & ROMAN, G. C. ( 1998). Neurocysticercosis: a Clinical Handbook. Swets and Zeitliger Publishers, Lisse.
EOM, K. S., JEON, H. K., KONG, Y., HWANG, U. W., YANG, Y., LI, X., XU, L., FENG, Z., PAWLOWSKI, Z. S. & RIM, H. J. ( 2002). Identification of Taenia asiatica in China: molecular, morphological, and epidemiological analysis of a Luzhai isolate. Journal of Parasitology 88, 758–764.CrossRefGoogle Scholar
FLISSER, A. ( 1994). Taeniasis and cysticercosis due to Taenia solium. In Progress in Clinical Parasitology ( ed. Sun, T.), pp. 77–116. CRC Press Florida.
GASSER, R. B., ZHU, X. & McMANUS, D. P. ( 1999). NADH dehydrogenase subunit 1 and cytochrome c oxidase subunit I sequences compared for members of the genus Taenia (Cestoda). International Journal for Parasitology 29, 1965–1970.CrossRefGoogle Scholar
HENNEBERG, R. ( 1912). Die tierischen Parasiten des Zentralnervensystems. I. Des Cysticercus cellulosae. In Handbuch der Neurologie Vol III. Spezielle Neurologie ( ed. Lewandowsky, M.), pp. 643–709. Verlag von Julinus Springer, Berlin.
JUNG, R. C., RODRIGUEZ, M. A., BEAVER, P. C., SCHENTHAL, J. E. & LEVY, R. W. ( 1981). Racemose cysticercus in human brain. A case report. American Journal of Tropical Medicine and Hygiene 30, 620–624.CrossRefGoogle Scholar
KIMURA, M. ( 1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16, 111–120.CrossRefGoogle Scholar
LOO, L. & BRAUDE, A. ( 1982). Cerebral cysticercosis in San Diego. A report of 23 cases and a review of the literature. Medicine 61, 341–359.CrossRefGoogle Scholar
McCORMICK, G. F. ( 1985). Cysticercosis-review of 230 patients. Bulletin of Clinical Neurosciences 50, 76–101.Google Scholar
McMANUS, D. P. & BOWLES, J. ( 1996). Molecular genetic approaches to parasite identification: their value in diagnostic parasitology and systematics. International Journal for Parasitology 26, 687–704.CrossRefGoogle Scholar
NAKAO, M., SAKO, Y., YOKOYAMA, N., FUKUNAGA, M. & ITO, A. ( 2000). Mitochondrial genetic code in cestodes. Molecular and Biochemical Parasitology 111, 415–424.CrossRefGoogle Scholar
NAKAO, M., OKAMOTO, M., SAKO, Y., YAMASAKI, H., NAKAYA, K. & ITO, A. ( 2002). A phylogenetic hypothesis for the distribution of two genotypes of the pig tapeworm Taenia solium worldwide. Parasitology 124, 657–662.CrossRefGoogle Scholar
OKIMOTO, R., MACFARLANE, J. L., CLARY, D. O. & WOLSTENHOLME, D. R. ( 1992). The mitochondrial genomes of two nematodes, Caenorhabditis elegans and Ascaris suum. Genetics 130, 471–498.Google Scholar
PAWLOWSKI, Z. S. ( 2002). Taenia solium: Basic and Transmission. In: Taenia solium Cysticercosis: from Basic to Clinical Science ( ed. Singh, G. & Prabhakar, S.), pp. 1–13. CABI Publishing, Oxon.
RABIELA, M. T., RIVAS, A., RODRIGUES, J., CASTILLO, S. & CACINO, F. M. ( 1982). Anatomo-pathological aspects of human brain cysticercosis. In Cysticercosis: present State of Knowledge and Perspectives ( ed. Flisser, A., Willms, K., Laclette, J. P. et al.), pp. 179–200. Academic Press, New York.
RABIELA, M. T., RIVAS, A. & FLISSER, A. ( 1989). Morphological types of Taenia solium cysticerci. Parasitology Today 5, 357–359.CrossRefGoogle Scholar
SAITOU, N. & NEI, M. ( 1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4, 406–425.Google Scholar
SLAIS, J. ( 1970). The Morphology and Pathogenicity of the Bladder worms: Cysticercus cellulosae and Cysticercus bovis. Dr W. Junk N.V. Publishers, The Hague.
THOMPSON, J. D., GIBSON, T. J., PLEWNIAK, F., JEANMOUGIN, F. & HIGGINS, D. G. ( 1997). The CLUSTAL-X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 25, 4876–4882.CrossRefGoogle Scholar
THOMPSON, R. C. A., LYMBERY, A. J. & CONSTANTINE, C. C. ( 1995). Variation in Echinococcus: towards a taxonomic revision of the genus. Advances in Parasitology 35, 146–176.CrossRefGoogle Scholar
VALKOUNOVA, J., ZDARSKA, Z. & SLAIS, J. ( 1992). Histochemistry of the acephalic form of Cysticercus cellulosae. Folia Parasitologica 39, 207–226.Google Scholar
VOGE, M. & BROWN, W. J. ( 1979). Fine structure of a racemose cysticercus from human brain. Journal of Parasitology 65, 262–266.CrossRefGoogle Scholar
WHITE, A. C. Jr. ( 2000). Neurocysticercosis: updates on epidemiology, pathogenesis, diagnosis, and management. Annual Review of Medicine 51, 187–206.CrossRefGoogle Scholar
ZARLENGA, D. S., McMANUS, D. P., FAN, P. C. & CROSS, J. H. ( 1991). Characterization and detection of a newly described Asian taeniid using cloned ribosomal DNA fragments and sequence amplification by the polymerase chain reaction. Experimental Parasitology 72, 174–183.CrossRefGoogle Scholar