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Molecular identification of Ancylostoma ceylanicum in the Philippines

Published online by Cambridge University Press:  24 August 2020

Oyime P. Aula Open the ORCID record for Oyime P. Aula [Opens in a new window] ,
Donald P. McManus Open the ORCID record for Donald P. McManus [Opens in a new window] ,
Kosala G. Weerakoon ,
Remigio Olveda ,
Allen G. Ross ,
Madeleine J. Rogers  and
Catherine A. Gordon Open the ORCID record for Catherine A. Gordon [Opens in a new window]
Oyime P. Aula
Affiliation:
Molecular Parasitology Laboratory, QIMR Berghofer Medical Research Institute, Queensland, Australia School of Chemistry and Molecular Biology, The University of Queensland, Queensland, Australia
Donald P. McManus
Affiliation:
Molecular Parasitology Laboratory, QIMR Berghofer Medical Research Institute, Queensland, Australia
Kosala G. Weerakoon
Affiliation:
Department of Parasitology, Faculty of Medicine and Allied Sciences, Rajarata University of Sri Lanka, Sri Lanka
Remigio Olveda
Affiliation:
Immunology Department, Research Institute of Tropical Medicine, Manila, the Philippines
Allen G. Ross
Affiliation:
Menzies Health Institute Queensland, Gold Coast, Australia icddr b, Dhaka, Bangladesh
Madeleine J. Rogers
Affiliation:
Molecular Parasitology Laboratory, QIMR Berghofer Medical Research Institute, Queensland, Australia School of Chemistry and Molecular Biology, The University of Queensland, Queensland, Australia
Catherine A. Gordon*
Affiliation:
Molecular Parasitology Laboratory, QIMR Berghofer Medical Research Institute, Queensland, Australia
*
Author for correspondence: Catherine A. Gordon, E-mail: Catherine.Gordon@qimrberghofer.edu.au
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Abstract

Hookworms are some of the most widespread of the soil-transmitted helminths (STH) with an estimated 438.9 million people infected. Until relatively recently Ancylostoma ceylanicum was regarded as a rare cause of hookworm infection in humans, with little public health relevance. However, recent advances in molecular diagnostics have revealed a much higher prevalence of this zoonotic hookworm than previously thought, particularly in Asia. This study examined the prevalence of STH and A. ceylanicum in the municipalities of Palapag and Laoang in the Philippines utilizing real-time polymerase chain reaction (PCR) on stool samples previously collected as part of a cross-sectional survey of schistosomiasis japonica. Prevalence of hookworm in humans was high with 52.8% (n = 228/432) individuals positive for any hookworm, 34.5% (n = 149/432) infected with Necator americanus, and 29.6% (n = 128/432) with Ancylostoma spp; of these, 34 were PCR-positive for A. ceylanicum. Considering dogs, 12 (n = 33) were PCR-positive for A. ceylanicum. This is the first study to utilize molecular diagnostics to identify A. ceylanicum in the Philippines with both humans and dogs infected. Control and elimination of this zoonotic hookworm will require a multifaceted approach including chemotherapy of humans, identification of animal reservoirs, improvements in health infrastructure, and health education to help prevent infection.

Keywords

Ancylostoma ceylanicumAncylostoma duodenaleNecator americanusPhilippinesreal-time PCR
Type
Research Article
Information
Parasitology , Volume 147 , Issue 14 , December 2020 , pp. 1718 - 1722
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Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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Footnotes

†

Deceased 2020

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