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A new technique for the extraction of Nematodirus battus eggs from sheep faeces

Published online by Cambridge University Press:  06 April 2009

W. J. Coadwell ,
Jean Martin  and
P. F. V. Ward
W. J. Coadwell
Affiliation:
Biochemistry Department, ARC Institute of Animal Physiology, Babraham, Cambridge CB2 4AT
Jean Martin
Affiliation:
Biochemistry Department, ARC Institute of Animal Physiology, Babraham, Cambridge CB2 4AT
P. F. V. Ward
Affiliation:
Biochemistry Department, ARC Institute of Animal Physiology, Babraham, Cambridge CB2 4AT
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Summary

Details are given of a method for the separation of the eggs of the nematode Nematodirus battus from sheep faeces. Faecal pellets obtained from worm-free lambs experimentally infected with N. battus were homogenized and passed through a graded series of metal sieves. The material retained in a 53, µm aperture sieve was transferred to a cylindrical column and the eggs were cleaned and separated from other suspended material by controlled differential flotation using tap water. A model experiment indicated that, within limits, the principle held for any size of tower. The density distribution for eggs at the morula stage of development was determined. Suggested flow rates for a specified tower are given.

Type
Research Article
Information
Parasitology , Volume 82 , Issue 2 , April 1981 , pp. 263 - 267
Copyright
Copyright © Cambridge University Press 1981

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References

REFERENCES

Gordon, H. M. & Whitlock, H. V. (1939). A new technique for counting nematode eggs in sheep faeces. Journal of the Council of Scientific and Industrial Research, Australia 12, 50–2.Google Scholar
Mapes, C. G. & Coop, R. L. (1970). The interactions of infections of Haemonchus contortus and Nematodirus battus in lambs. Journal of Comparative Pathology 80, 123–36.CrossRefGoogle ScholarPubMed
Wassall, D. A. & Denham, D. A. (1969). A method for the recovery of nematode eggs from faeces. Parasitology 59, 279–82.CrossRefGoogle ScholarPubMed