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An advanced lentil backcross population developed from a cross between Lens culinaris × L. ervoides for future disease resistance and genomic studies

Published online by Cambridge University Press:  21 April 2021

Tadesse S. Gela Open the ORCID record for Tadesse S. Gela [Opens in a new window] ,
Stanley Adobor Open the ORCID record for Stanley Adobor [Opens in a new window] ,
Hamid Khazaei Open the ORCID record for Hamid Khazaei [Opens in a new window]  and
Albert Vandenberg
Tadesse S. Gela*
Affiliation:
Department of Plant Sciences, University of Saskatchewan, 51 Campus Drive, Saskatoon, Saskatchewan, S7N 5A8, Canada
Stanley Adobor
Affiliation:
Department of Plant Sciences, University of Saskatchewan, 51 Campus Drive, Saskatoon, Saskatchewan, S7N 5A8, Canada
Hamid Khazaei
Affiliation:
Department of Plant Sciences, University of Saskatchewan, 51 Campus Drive, Saskatoon, Saskatchewan, S7N 5A8, Canada
Albert Vandenberg*
Affiliation:
Department of Plant Sciences, University of Saskatchewan, 51 Campus Drive, Saskatoon, Saskatchewan, S7N 5A8, Canada
*
*Corresponding authors. E-mail: tadesse.gela@usask.ca; bert.vandenbarg@usask.ca
*Corresponding authors. E-mail: tadesse.gela@usask.ca; bert.vandenbarg@usask.ca
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Abstract

Genetically accessible variation to some of the abiotic and biotic stresses are limited in the cultivated lentil (Lens culinaris Medik.) germplasm. Introgression of novel alleles from its wild relative species will be useful for enhancing the genetic improvement of the crop. L. ervoides, one of the wild relatives of lentil, is a proven source of disease resistance for the crop. Here we introduce a lentil advanced backcross (LABC-01) population developed in cultivar ‘CDC Redberry’ background, based on L. ervoides alleles derived from an interspecific recombinant inbred population, LR-59-81. Two-hundred and seventeen individuals of the LABC-01 population at BC2F3:4 generation were screened for the race 0 of anthracnose (Colletotrichum lentis) and stemphylium blight (Stemphylium botryosum) under controlled conditions. The population showed significant variations for both diseases and the transfer of resistance alleles into the elite cultivar was evident. It also segregated for other traits such as days to flowering, seed coat colour, seed coat pattern and flower colour. Overall, we showed that LABC-01 population can be used in breeding programmes worldwide to improve disease resistance and will be available as a valuable genetic resource for future genetic analysis of desired loci introgressed from L. ervoides.

Keywords

biotic stressescrop wild relativesLens sppmapping populationpre-breeding
Type
Research Article
Information
Plant Genetic Resources , Volume 19 , Issue 2 , April 2021 , pp. 167 - 173
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of NIAB

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References

Adobor, S, Podder, R, Banniza, S and Vandenberg, A (2020) Evaluation of resistance to stemphylium blight in interspecific recombinant inbred lines derived from Lens culinaris×Lens ervoides. Plant Genetic Resources 18: 251–258.CrossRefGoogle Scholar
Banniza, S, Warale, R, Meant, J, Cohen-Skali, A, Armstrong-Cho, C and Bhadauria, V (2018) The long path to understanding the host-pathogen interactions of Colletotrichum lentis on lentil. Canadian Journal of Plant Pathology 40: 199–209.CrossRefGoogle Scholar
Barilli, E, Moral, J, Aznar-Fernández, T and Rubiales, D (2020) Resistance to anthracnose (Colletotrichum lentis, race 0) in Lens spp. germplasm. Agronomy 10: 1799.CrossRefGoogle Scholar
Bett, K, Ramsay, L, Chan, C, Sharpe, A, Cook, D, Penmetsa, RV, Chang, P, Coyne, C, McGee, R, Main, D, Edwards, D, Kaur, S and Vandenberg, A (2016) Lentil 1.0 and beyond. In: PAG XXIV: Plant and animal genomics conference, 8–13 January 2016, San Diego, California, USA.Google Scholar
Bhadauria, V, Ramsay, L, Bett, KE and Banniza, S (2017) QTL mapping reveals genetic determinants of fungal disease resistance in the wild lentil species Lens ervoides. Scientific Reporters 7: 3231.CrossRefGoogle ScholarPubMed
Bhanu, AN, Gokidi, Y and Singh, MN (2017) Advanced backcross QTL method: a brief overview. Trends in Biosciences 10: 20–25.Google Scholar
Bucak, B, Bett, K, Banniza, S and Vandenberg, A (2014) Transfer of resistance to broomrape (Orobanche crenata) from Lens ervoides to cultivated lentil. In: 6th International Food Legume Research Conference (IFLRCVI), 7–11 July 2014, Saskatoon, Canada, p. 62.Google Scholar
Buchwaldt, L, Anderson, KL, Morrall, RAA, Gossen, BD and Bernier, CC (2004) Identification of lentil germplasm resistant to Colletotrichum truncatum and characterization of two pathogen races. Phytopathology 94: 236–243.CrossRefGoogle Scholar
Caudillo-Ruiz, KB (2016) Characterization of the Stemphylium Blight Pathogens and Their Effect on Lentil Yield (MSc dissertation). University of Saskatchewan, Saskatoon, Canada.Google Scholar
Chen, L (2018) Assessing Impacts of Crop-Wild Introgression in Lentil Using Interspecific Lens Species Recombinant Inbred Line Populations (PhD dissertation), University of Saskatchewan, Saskatoon, Canada.Google Scholar
Dempewolf, H, Baute, G, Anderson, J, Kilian, B, Smith, C and Guarino, L (2017) Past and future use of wild relatives in crop breeding. Crop Science 57: 1070–1082.CrossRefGoogle Scholar
Eduardo, I, Arús, P and Monforte, AJ (2005) Development of a genomic library of near isogenic lines (NILs) in melon (Cucumis melo L.) from the exotic accession PI161375. Theoretical and Applied Genetics 112: 139–148.CrossRefGoogle ScholarPubMed
Erskine, W, Adham, Y and Holly, L (1989) Geographic distribution of variation in quantitative traits in a world lentil collection. Euphytica 43: 97–103.CrossRefGoogle Scholar
Erskine, W, Tufail, M, Russell, A, Tyagi, MC, Rahman, MM and Saxena, MC (1994) Current and future strategies in breeding lentil for resistance to biotic and abiotic stresses. Euphytica 73: 127–135.CrossRefGoogle Scholar
Fiala, JV, Tullu, A, Banniza, S, Séguin-Swartz, G and Vandenberg, A (2009) Interspecies transfer of resistance to anthracnose in lentil (Lens culinaris Medik.). Crop Science 49: 825–830.CrossRefGoogle Scholar
Food and Agriculture Organization of the United Nations (2021) FAOSTAT, Crops. http://faostat3.fao.org (accessed 9 January 2021).Google Scholar
Frischa, M, Bohna, M and Melchinger, AE (1999) Comparison of selection strategies for marker-assisted backcrossing of a gene. Crop Science 39: 1295–1301.CrossRefGoogle Scholar
Fulton, TM, Beck-Bunn, T, Emmatty, D, Eshed, Y, Lopez, J, Petiard, V, Uhlig, J, Zamir, D and Tanksley, SD (1997) QTL analysis of an advanced backcross of Lycopersicon peruvianum to the cultivated tomato and comparisons with QTLs found in other wild species. Theoretical and Applied Genetics 95: 881–894.CrossRefGoogle Scholar
Gela, TS, Banniza, S and Vandenberg, A (2020) Lack of effective resistance to the virulent race of Colletotrichum lentis in Lens culinaris Medikus subsp. culinaris. Plant Genetic Resources 18: 81–87.CrossRefGoogle Scholar
Gorim, LY and Vandenberg, A (2017) Evaluation of wild lentil species as genetic resources to improve drought tolerance in cultivated lentil. Frontiers in Plant Science 8: 1129.CrossRefGoogle ScholarPubMed
Gujaria-Verma, N, Vail, S, Carrasquilla-Garcia, N, Penmetsa, RV, Cook, DR, Farmer, AD, Vandenbeg, A and Bett, KE (2014) Genetic mapping of legume orthologs reveals high conservation of synteny between lentil species and the sequenced genomes of medicago and chickpea. Frontiers in Plant Science 5: 676.CrossRefGoogle ScholarPubMed
Gupta, D and Sharma, SK (2006) Evaluation of wild Lens taxa for agro-morphological traits, fungal diseases and moisture stress in northwestern Indian hills. Genetic Resources and Crop Evolution 53: 1233–1241.CrossRefGoogle Scholar
Hajjar, R and Hodgkin, T (2007) The use of wild relatives in crop improvement: a survey of developments over the last 20 years. Euphytica 156: 1–13.CrossRefGoogle Scholar
Khazaei, H, Caron, CT, Diapari, M, Fedoruk, M, Vandenberg, A, Coyne, CJ, McGee, R and Bett, KE (2016) Genetic diversity of cultivated lentil (Lens culinaris Medik.) and its relation to the world's agro-ecological zones. Frontiers in Plant Science 7: 1093.CrossRefGoogle ScholarPubMed
Ladizinsky, G, Braun, D, Goshen, D and Muehlbauer, F (1984) The biological species of the genus Lens L. Botanical Gazette 145: 253–261.CrossRefGoogle Scholar
Maxted, N and Kell, SP (2009) Establishment of a global network for the in situ conservation of crop wild relatives: status and needs. Background study paper no. 39. Commission on Genetic Resources for Food and Agriculture, Food and Agriculture Organization of the United Nations, Rome, Italy. Available at http://fao.org/3/a-i1500e/i1500e18d.pdf (accessed 2 February 2021).Google Scholar
Maxted, N, Kell, S, Ford-Lloyd, B, Dulloo, E and Toledo, Á (2012) Toward the systematic conservation of global crop wild relative diversity. Crop Science 52: 774–785.CrossRefGoogle Scholar
Ogutcen, E, Ramsay, L, von Wettberg, EB and Bett, KE (2018) Capturing variation in Lens (Fabaceae): development and utility of an exome capture array for lentil. Applications in Plant Sciences 6: e01165.CrossRefGoogle ScholarPubMed
Podder, R (2018) Iron Biofortification and Fortification of Lentil (Lens culinaris Medik.) (PhD dissertation). University of Saskatchewan, Saskatoon, Canada.CrossRefGoogle Scholar
Podder, R, Banniza, S and Vandenberg, A (2013) Screening of wild and cultivated lentil germplasm for resistance to stemphylium blight. Plant Genetic Resources 11: 26–35.CrossRefGoogle Scholar
Pratap, A, Das, A, Kumar, S and Gupta, S (2021) Current perspectives on introgression breeding in food legumes. Frontiers in Plant Science 11:589189.CrossRefGoogle ScholarPubMed
Prohens, J, Gramazio, P, Plazas, M, Dempewolf, H, Kilian, B, Díez, MJ, Fita, A, Herraiz, FJ, Rodríguez-Burruezo, A, Soler, S, Knapp, S and Vilanova, S (2017) Introgressiomics: a new approach for using crop wild relatives in breeding for adaptation to climate change. Euphytica 213: 1–20.CrossRefGoogle Scholar
SAS Institute, Inc. (2011) SAS Language and Procedure: Usage Version 9.4. Cary, NC, USA: SAS Institute, Inc.Google Scholar
Schmalenbach, I, Korber, N and Pillen, K (2008) Selecting a set of wild barley introgression lines and verification of QTL effects for resistance to powdery mildew and leaf rust. Theoretical and Applied Genetics 117: 1093–1106.CrossRefGoogle ScholarPubMed
Singh, M, Bisht, IS, Kumar, S, Dutta, M and Chander, K (2014) Global wild annual Lens collection: a potential resource for lentil genetic base broadening and yield enhancement. PLoS ONE 9: e107781.CrossRefGoogle ScholarPubMed
Singh, M, Rana, JC, Singh, B, Kumar, S, Saxena, DR, Saxena, A, Rizvi, AH and Sarker, A (2017) Comparative agronomic performance and reaction to fusarium wilt of Lens culinaris×L. orientalis and L. culinaris×L. ervoides derivatives. Frontiers in Plant Science 8: 1162.CrossRefGoogle ScholarPubMed
Slinkard, AE (1981) Eston lentil. Canadian Journal of Plant Science 61: 733–734.CrossRefGoogle Scholar
Tadmor, Y, Zamir, D and Ladizinsky, G (1987) Genetic mapping of an ancient translocation in the genus Lens. Theoretical and Applied Genetics 73: 883–892.CrossRefGoogle ScholarPubMed
Taguchi-Shiobara, F, Ozaki, H, Sato, H, Maeda, H, Kojima, Y, Ebitani, T and Yano, M (2013) Mapping and validation of QTLs for rice sheath blight resistance. Breeding Science 63: 301–308.CrossRefGoogle ScholarPubMed
Tanksley, SD and Nelson, JC (1996) Advanced backcross QTL analysis: a method for the simultaneous discovery and transfer of valuable QTLs from unadapted germplasm into elite breeding lines. Theoretical and Applied Genetics 92: 191–203.CrossRefGoogle ScholarPubMed
Tanksley, SD, Young, ND, Paterson, AH and Bonierbale, MW (1989) RFLP mapping in plant breeding: new tools for an old science. Nature Biotechnology 7: 257–264.CrossRefGoogle Scholar
Tian, F, Li, DJ, Fu, Q, Zhu, FZ, Fu, YC, Wang, XK and Sun, CQ (2006) Construction of introgression lines carrying wild rice (Oryza rufipogon Griff.) segments in cultivated rice (Oryza sativa L.) background and characterization of introgressed segments associated with yield-related traits. Theoretical and Applied Genetics 112: 570–580.CrossRefGoogle ScholarPubMed
Tullu, A, Buchwaldt, L, Lulsdorf, M, Banniza, S, Barlow, B, Slinkard, AE, Sarker, A, Tar'an, B, Warkentin, T and Vandenberg, A (2006) Sources of resistance to anthracnose (Colletotrichum truncatum) in wild Lens species. Genetic Resources and Crop Evolution 53: 111–119.CrossRefGoogle Scholar
Tullu, A, Banniza, S, Tar'an, B, Warkentin, T and Vandenberg, A (2010) Sources of resistance to ascochyta blight in wild species of lentil (Lens culinaris Medik.). Genetic Resources and Crop Evolution 57: 1053–1063.CrossRefGoogle Scholar
Tullu, A, Diederichsen, A, Suvorova, G and Vandenberg, A (2011) Genetic and genomic resources of lentil: status, use and prospects. Plant Genetic Resources 9: 19–29.CrossRefGoogle Scholar
Tullu, A, Bett, K, Banniza, S, Vail, S and Vandenberg, A (2013) Widening the genetic base of cultivated lentil through hybridization of Lens culinaris ‘Eston’ and L. ervoides accession IG 72815. Canadian Journal of Plant Science 93: 1037–1047.CrossRefGoogle Scholar
Vail, SL (2010) Interspecific-Derived and Juvenile Resistance to Anthracnose in Lentil (PhD dissertation). University of Saskatchewan, Saskatoon, Canada.Google Scholar
Vail, S, Strelioff, JV, Tullu, A and Vandenberg, A (2012) Field evaluation of resistance to Colletotrichum truncatum in Lens culinaris, Lens ervoides, and Lens ervoides×Lens culinaris derivatives. Field Crops Research 126: 145–151.CrossRefGoogle Scholar
Vandenberg, A, Kiehn, FA, Vera, C, Gaudiel, R, Buchwaldt, L, Dueck, S, Morrall, RAA, Wahab, J and Slinkard, AE (2002) CDC Glamis lentil. Canadian Journal of Plant Science 82: 103–104.CrossRefGoogle Scholar
Vandenberg, A, Banniza, S, Warkentin, TD, Ife, S, Barlow, B, McHale, S, Brolley, B, Gan, Y, McDonald, C, Bandara, M and Dueck, S (2006) CDC Redberry lentil. Canadian Journal of Plant Science 86: 497–498.CrossRefGoogle Scholar
Van Oss, H, Aron, Y and Ladizinsky, G (1997) Chloroplast DNA variation and evolution in the genus Lens Mill. Theoretical and Applied Genetics 94:452–457.CrossRefGoogle Scholar
Wong, MML, Gujaria-Verma, N, Ramsay, L, Yuan, HY, Caron, C, Diapari, M, Vandenberg, A and Bett, KE (2015) Classification and characterization of species within the genus Lens using genotyping-by-sequencing (GBS). PLOS ONE 10: e0122025.CrossRefGoogle Scholar
Yuan, HY, Saha, S, Vandenberg, A and Bett, KE (2017) Flowering and growth responses of cultivated lentil and wild Lens germplasm toward the differences in red to far-red ratio and photosynthetically active radiation. Frontiers in Plant Science 8: 386.CrossRefGoogle ScholarPubMed
Yun, SJ, Gyenis, L, Bossolini, E, Hayes, PM, Matus, I, Smith, KP, Steffenson, BJ, Tuberosa, R and Muehlbauer, GJ (2006) Validation of quantitative trait loci for multiple disease resistance in barley using advanced backcross lines developed with a wild barley. Crop Science 46: 1179–1186.CrossRefGoogle Scholar
Zamir, D (2001) Improving plant breeding with exotic genetic libraries. Nature Reviews Genetics 2: 983–989.CrossRefGoogle ScholarPubMed
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